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protein coding gene - dcp2 (SPAC19A8.12) - mRNA decapping complex catalytic subunit Dcp2

Gene summary

Standard name
dcp2
Systematic ID
SPAC19A8.12
Product
mRNA decapping complex catalytic subunit Dcp2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O13828
ORFeome ID
46/46G04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2462202..2465047 reverse strand

Annotation

GO biological process

GO:0000290 - deadenylation-dependent decapping of nuclear-transcribed mRNA

References:

GO:0110156 - mRNA methylguanosine-cap decapping

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0010494 - cytoplasmic stress granule

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0000932 - P-body

References:

GO:0098745 - RNA decapping complex

References:

GO molecular function

GO:0140932 - 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase activity

References:

GO:0005524 - ATP binding

References:

GO:0000287 - magnesium ion binding

References:

GO:0030145 - manganese ion binding

References:

GO:0098808 - mRNA cap binding

References:

GO:0005515 - protein binding

References:

GO:0003723 - RNA binding

References:

GO:0003727 - single-stranded RNA binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0003940 - abolished m7G(5')pppN diphosphatase activity

References:

Genotypes:

FYPO:0003749 - abolished positive regulation of m7G(5')pppN diphosphatase activity

References:

Genotypes:

FYPO:0006001 - abolished protein localization to P-bodies

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0003941 - decreased m7G(5')pppN diphosphatase activity

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0002134 - decreased protein-RNA interaction

References:

Genotypes:

FYPO:0001896 - enlarged P-bodies

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0003942 - normal m7G(5')pppN diphosphatase activity

References:

Genotypes:

FYPO:0004083 - normal protein level

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0001895 - P-bodies present in increased numbers

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00293NUDIXNUDIX_hydrolase_domPfam
PF05026DCP2DCP2_BoxAPfam
cd03672NUDIX_Dcp2p_Nudt20Dcp2_NUDIXCDD
PS00893NUDIX_BOXNUDIX_hydrolase_CSPROSITE patterns
PS51462NUDIXNUDIX_hydrolase_domPROSITE profiles
SM01125DCP2DCP2_BoxASMART
G3DSA:1.10.10.1050:FF:000003CATH-FunFam
G3DSA:3.90.79.10:FF:000003CATH-FunFam
G3DSA:1.10.10.1050DCP2_BoxA_sfCATH-Gene3D
G3DSA:3.90.79.10NUDIX_hydrolase-like_dom_sfCATH-Gene3D
SSF140586DCP2_BoxA_sfSUPERFAMILY
SSF55811NUDIX_hydrolase-like_dom_sfSUPERFAMILY
PTHR23114PANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:23319050 - Pdc1 functions in the assembly of P bodies in Schizosaccharomyces pombe.
Wang CY et al. Mol Cell Biol 2013 Mar;33(6):1244-53
PMID:15671491 - Decapping reaction of mRNA requires Dcp1 in fission yeast: its characterization in different species from yeast to human.
Sakuno T et al. J Biochem 2004 Dec;136(6):805-12
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:24862735 - In vitro reconstitution of a cellular phase-transition process that involves the mRNA decapping machinery.
Fromm SA et al. Angew Chem Int Ed Engl 2014 Jul 07;53(28):7354-9
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:21098141 - Cellular stress induces cytoplasmic RNA granules in fission yeast.
Nilsson D et al. RNA 2011 Jan;17(1):120-33
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:28533364 - Changes in conformational equilibria regulate the activity of the Dcp2 decapping enzyme.
Wurm JP et al. Proc Natl Acad Sci U S A 2017 Jun 06;114(23):6034-6039
PMID:17502373 - Fission yeast Rnf4 homologs are required for DNA repair.
Kosoy A et al. J Biol Chem 2007 Jul 13;282(28):20388-94
PMID:19336419 - Cotranscriptional recruitment of the nuclear poly(A)-binding protein Pab2 to nascent transcripts and association with translating mRNPs.
Lemieux C et al. Nucleic Acids Res 2009 Jun;37(10):3418-30
PMID:31690658 - Pat1 activates late steps in mRNA decay by multiple mechanisms.
Lobel JH et al. Proc Natl Acad Sci U S A 2019 Nov 19;116(47):23512-23517
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:32513655 - Pdc2/Pat1 increases the range of decay factors and RNA bound by the Lsm1-7 complex.
Lobel JH et al. RNA 2020 Oct;26(10):1380-1388
PMID:20547592 - Vgl1, a multi-KH domain protein, is a novel component of the fission yeast stress granules required for cell survival under thermal stress.
Wen WL et al. Nucleic Acids Res 2010 Oct;38(19):6555-66
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:27183195 - Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation.
Valkov E et al. Nat Struct Mol Biol 2016 Jun;23(6):574-9
GO_REF:0000002 - Comments
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:22085934 - The structural basis of Edc3- and Scd6-mediated activation of the Dcp1:Dcp2 mRNA decapping complex.
Fromm SA et al. EMBO J 2012 Jan 18;31(2):279-90
PMID:32071154 - Fission Yeast Puf2, a Pumilio and FBF Family RNA-Binding Protein, Links Stress Granules to Processing Bodies.
Hsiao WY et al. Mol Cell Biol 2020 Apr 13;40(9)
PMID:42014425 - Conserved and divergent features of human mRNA decapping revealed by biochemical reconstitution.
Simko EAJ et al. Nat Commun 2026 Apr 21;17(1)
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:28472520 - A synergistic network of interactions promotes the formation of in vitro processing bodies and protects mRNA against decapping.
Schütz S et al. Nucleic Acids Res 2017 Jun 20;45(11):6911-6922
PMID:16341225 - Crystal structure and functional analysis of Dcp2p from Schizosaccharomyces pombe.
She M et al. Nat Struct Mol Biol 2006 Jan;13(1):63-70
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:18280239 - Structural basis of dcp2 recognition and activation by dcp1.
She M et al. Mol Cell 2008 Feb 15;29(3):337-49
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:27354705 - The S. pombe mRNA decapping complex recruits cofactors and an Edc1-like activator through a single dynamic surface.
Wurm JP et al. RNA 2016 Sep;22(9):1360-72
PMID:27694842 - Structural basis of mRNA-cap recognition by Dcp1-Dcp2.
Mugridge JS et al. Nat Struct Mol Biol 2016 Nov;23(11):987-994
PMID:28031482 - Involvement of fission yeast Pdc2 in RNA degradation and P-body function.
Wang CY et al. RNA 2017 Apr;23(4):493-503
PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.
Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:29101277 - Application of a Schizosaccharomyces pombe Edc1-fused Dcp1-Dcp2 decapping enzyme for transcription start site mapping.
Paquette DR et al. RNA 2018 Feb;24(2):251-257
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:22323607 - Interdomain dynamics and coactivation of the mRNA decapping enzyme Dcp2 are mediated by a gatekeeper tryptophan.
Floor SN et al. Proc Natl Acad Sci U S A 2012 Feb 21;109(8):2872-7
PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.
Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7
PMID:25543137 - Dicer and Hsp104 function in a negative feedback loop to confer robustness to environmental stress.
Oberti D et al. Cell Rep 2015 Jan 06;10(1):47-61
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963