protein coding gene - usp101 (SPAC19A8.13) - U1 snRNP-associated protein Usp101

Gene summary

Standard name: usp101
Systematic ID: SPAC19A8.13
Product: U1 snRNP-associated protein Usp101
Organism: Schizosaccharomyces pombe (fission yeast)
Synonyms: snp1
UniProt ID: O13829
ORFeome ID: 09/09G10
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome I: 2461151..2462182 reverse strand

Annotation

GO biological process

GO:0000395 - mRNA 5'-splice site recognition

References:

GO_REF:0000024

GO cellular component

GO:0000243 - commitment complex

References:

GO_REF:0000024

GO:0005829 - cytosol

References:

PMID:16823372

GO:0005634 - nucleus

References:

PMID:16823372

GO:0071011 - precatalytic spliceosome

References:

GO_REF:0000033

GO:0005685 - U1 snRNP

References:

PMID:17264129

GO:0071004 - U2-type prespliceosome

References:

GO_REF:0000033

GO molecular function

GO:0003729 - mRNA binding

References:

GO_REF:0000033

GO:0030619 - U1 snRNA binding

References:

GO_REF:0000033

Modification

MOD:00046 - O-phospho-L-serine

References:

PMID:29996109

Protein features

PBO:0111764 - rrm RNA recognition motif

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

PMID:27984744

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

PMID:23697806

Genotypes:

usp101delta

FYPO:0001490 - inviable elongated vegetative cell

References:

PMID:23697806

Genotypes:

usp101delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

usp101delta

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

PMID:23697806

Genotypes:

usp101delta

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF12220	U1snRNP70_N	U1snRNP70_N	PFAM
PF00076	RRM_1	RRM_dom	PFAM
cd12236	RRM_snRNP70	snRNP70_RRM	CDD
PS50102	RRM	RRM_dom	PROSITE_PROFILES
SM00360	rrm1_1	RRM_dom	SMART
G3DSA:3.30.70.330:FF:000132			FUNFAM
SSF54928	RNA-binding domain, RBD	RBD_domain_sf	SUPERFAMILY
G3DSA:3.30.70.330		Nucleotide-bd_a/b_plait_sf	GENE3D
PTHR13952	U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD	U1_U11-U12_snRNP_70-35kDa	PANTHER
Coil	Coil		COILS
mobidb-lite-Disorder	disorder_prediction		MOBIDB-Disorder
mobidb-lite-Glycine-rich	disorder_prediction		MOBIDB-Glycine-rich
mobidb-lite-Polyampholyte	disorder_prediction		MOBIDB-Polyampholyte

Orthologs

SNRNP35 (HGNC:30852) Homo sapiens
usp101 (SJAG_05009) Schizosaccharomyces japonicus
SNP1 (YIL061C) Saccharomyces cerevisiae

References / Literature

PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:17264129 - Proteomic analysis of the U1 snRNP of Schizosaccharomyces pombe reveals three essential organism-specific proteins.: Newo AN et al. Nucleic Acids Res 2007;35(5):1391-401
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.: Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.: Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:28947618 - Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.: Thakran P et al. EMBO J 2018 Jan 04;37(1):89-101
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.: Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:28446597 - The histone variant H2A.Z promotes splicing of weak introns.: Nissen KE et al. Genes Dev 2017 Apr 01;31(7):688-701
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.: Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.: Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.: Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.: Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053