protein coding gene - ttc27 (SPAC19B12.01) - eEF2 folding chaperone Ttc27

Gene summary

Standard name: ttc27
Systematic ID: SPAC19B12.01
Product: eEF2 folding chaperone Ttc27
Organism: Schizosaccharomyces pombe (fission yeast)
Synonyms: SPAC4F10.21, ceo5
UniProt ID: O36033
ORFeome ID: 38/38D07
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome I: 4875387..4878120 forward strand

Annotation

GO biological process

GO:0051083 - 'de novo' cotranslational protein folding

References:

PMID:41806041

GO cellular component

GO:0005829 - cytosol

References:

PMID:16823372

GO:0005634 - nucleus

References:

PMID:16823372

GO molecular function

GO:0044183 - protein folding chaperone

References:

PMID:41806041

Modification

MOD:01148 - ubiquitinylated lysine

References:

PMID:37970674

Protein features

PBO:0111755 - TPR repeat protein

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

References:

PMID:37162093

Genotypes:

ceo5+(wild type)[Knockdown]

FYPO:0000017 - elongated cell

References:

PMID:37162093

Genotypes:

ceo5+(wild type)[Knockdown]

FYPO:0002420 - inviable after spore germination, single cell division, abnormal cell shape

References:

PMID:23697806

Genotypes:

ttc27delta

FYPO:0002151 - inviable spore

References:

PMID:23697806

Genotypes:

ttc27delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

ttc27delta

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF13181	TPR_8	TPR_rpt	PFAM
PS50005	TPR	TPR_rpt	PROSITE_PROFILES
SM00028	tpr_5	TPR_rpt	SMART
G3DSA:1.25.40.10:FF:000884			FUNFAM
SSF48452	TPR-like	TPR-like_helical_dom_sf	SUPERFAMILY
G3DSA:1.25.40.10	Tetratricopeptide repeat domain	TPR-like_helical_dom_sf	GENE3D
PTHR16193	TETRATRICOPEPTIDE REPEAT PROTEIN 27	TTC27/Emw1	PANTHER
mobidb-lite-Disorder	disorder_prediction		MOBIDB-Disorder

Orthologs

TTC27 (HGNC:25986) Homo sapiens
ttc27 (SJAG_01291) Schizosaccharomyces japonicus
EMW1 (YNL313C) Saccharomyces cerevisiae

References / Literature

PMID:22119525 - SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.: Singh NS et al. Curr Biol 2011 Dec 06;21(23):1968-78
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37162093 - Genetic knockdown of genes that are obscure, conserved and essential using CRISPR interference methods in the fission yeast S. pombe.: Ishikawa K et al. J Cell Sci 2023 May 01;136(9)
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.: Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:41806041 - Emw1/TTC27 is a chaperone required for folding of the eukaryotic elongation factor 2.: Yang M et al. Cell Mol Life Sci 2026 Mar 10;
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.: Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.: Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323