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protein coding gene - fcp1 (SPAC19B12.05c) - RNA polymerase II CTD phosphatase Fcp1

Gene summary

Standard name
fcp1
Systematic ID
SPAC19B12.05c
Product
RNA polymerase II CTD phosphatase Fcp1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9P376
ORFeome ID
38/38D09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4890740..4893628 reverse strand

Annotation

Catalytic activity attributes

PBO:0001083 - active site mapping

References:

Disease association

MONDO:0011402 - congenital cataracts-facial dysmorphism-neuropathy syndrome

References:

GO biological process

GO:0006357 - regulation of transcription by RNA polymerase II

References:

GO:0023052 - signaling

References:

GO cellular component

GO:0005634 - nucleus

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GO molecular function

GO:0005515 - protein binding

References:

GO:0008420 - RNA polymerase II CTD heptapeptide repeat phosphatase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0002680 - increased protein phosphorylation

References:

Genotypes:

FYPO:0004422 - normal protein phosphorylation

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Genotypes:

Protein features

PBO:0111779 - BRCT domain

Qualitative gene expression

PomGeneEx:0000020 - protein level unchanged

References:

PomGeneEx:0000021 - protein present

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001014 - abnormal membrane organization during nitrogen starvation

References:

Genotypes:

FYPO:0004303 - abolished phosphatase activity

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Genotypes:

FYPO:0004069 - abolished phosphorylation of RNA polymerase II C-terminal domain serine 2 residues

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Genotypes:

FYPO:0008061 - abolished phosphorylation of RNA polymerase II C-terminal domain serine 5 residues during vegetative growth

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Genotypes:

FYPO:0004363 - altered level of stress responsive gene mRNA during vegetative growth

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Genotypes:

FYPO:0004306 - decreased CTD phosphatase activity

References:

Genotypes:

FYPO:0004304 - decreased phosphatase activity

References:

Genotypes:

FYPO:0004067 - decreased phosphorylation of RNA polymerase II C-terminal domain serine 2 residues during vegetative growth

References:

Genotypes:

FYPO:0004068 - decreased phosphorylation of RNA polymerase II C-terminal domain serine 5 residues during vegetative growth

References:

Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0005041 - increased phosphorylation of RNA polymerase II C-terminal domain serine 2 residues during vegetative growth

References:

Genotypes:

FYPO:0005184 - increased phosphorylation of RNA polymerase II C-terminal domain serine 5 residues during vegetative growth

References:

Genotypes:

FYPO:0002113 - inviable curved vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0005061 - normal phosphorylation of RNA polymerase II C-terminal domain serine 5 residues during vegetative growth

References:

Genotypes:

FYPO:0000107 - sensitive to latrunculin A

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

Protein features

IDNameInterPro nameDB name
PF03031NIFFCP1_domPfam
PF12738PTCB-BRCTBRCT_domPfam
cd07521HAD_FCP1-likeCDD
cd17729BRCT_CTDP1CDD
PS50172BRCTBRCT_domPROSITE profiles
PS50969FCP1FCP1_domPROSITE profiles
SM00577CPDcFCP1_domSMART
G3DSA:3.40.50.1000:FF:000308CATH-FunFam
G3DSA:3.40.50.10190:FF:000049CATH-FunFam
G3DSA:1.10.287.10CATH-Gene3D
G3DSA:3.40.50.1000HAD_sfCATH-Gene3D
G3DSA:3.40.50.10190BRCT_dom_sfCATH-Gene3D
SSF52113BRCT_dom_sfSUPERFAMILY
SSF56784HAD-like_sfSUPERFAMILY
PTHR23081Fcp1PANTHER
TIGR02250FCP1_eukFCP1_eukNCBIFAM
SFLDG01124C0.1:_RNA_Pol_CTD_Phosphatase_RNA_Pol_CTD_PhosphataseSFLD
SFLDS00003Haloacid_DehalogenaseSFLD
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMobiDB-Negative-Polyelectrolyte
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:22144909 - The fission yeast stress-responsive MAPK pathway promotes meiosis via the phosphorylation of Pol II CTD in response to environmental and feedback cues.
Sukegawa Y et al. PLoS Genet 2011 Dec;7(12):e1002387
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:12556522 - Defining the active site of Schizosaccharomyces pombe C-terminal domain phosphatase Fcp1.
Hausmann S et al. J Biol Chem 2003 Apr 18;278(16):13627-32
PMID:15616156 - Tfg3, a subunit of the general transcription factor TFIIF in Schizosaccharomyces pombe, functions under stress conditions.
Kimura M et al. Nucleic Acids Res 2004;32(22):6706-15
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000051 - S. pombe keyword mapping
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:19026779 - The structure of Fcp1, an essential RNA polymerase II CTD phosphatase.
Ghosh A et al. Mol Cell 2008 Nov 21;32(4):478-90
PMID:17502918 - A cyclin-dependent kinase that promotes cytokinesis through modulating phosphorylation of the carboxy terminal domain of the RNA Pol II Rpb1p sub-unit.
Karagiannis J et al. PLoS One 2007 May 09;2(5):e433
PMID:11839823 - Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II.
Kimura M et al. Mol Cell Biol 2002 Mar;22(5):1577-88
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:19366728 - Genetic control of cellular quiescence in S. pombe.
Sajiki K et al. J Cell Sci 2009 May 01;122(Pt 9):1418-29
PMID:25883047 - Genetic and structural analysis of the essential fission yeast RNA polymerase II CTD phosphatase Fcp1.
Schwer B et al. RNA 2015 Jun;21(6):1135-46
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:29899453 - A Cdk9-PP1 switch regulates the elongation-termination transition of RNA polymerase II.
Parua PK et al. Nature 2018 Jun;558(7710):460-464
PMID:15620689 - Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit.
Mitsuzawa H et al. FEBS Lett 2005 Jan 03;579(1):48-52
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:14701811 - Schizosaccharomyces pombe carboxyl-terminal domain (CTD) phosphatase Fcp1: distributive mechanism, minimal CTD substrate, and active site mapping.
Hausmann S et al. J Biol Chem 2004 Mar 19;279(12):10892-900
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:11934898 - Characterization of the CTD phosphatase Fcp1 from fission yeast. Preferential dephosphorylation of serine 2 versus serine 5.
Hausmann S et al. J Biol Chem 2002 Jun 14;277(24):21213-20
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105