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protein coding gene - atg4 (SPAC19B12.08) - Atg8 deconjugator Atg4

Gene summary

Standard name
atg4
Systematic ID
SPAC19B12.08
Product
Atg8 deconjugator Atg4
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ppp80
UniProt ID
Q9P373
ORFeome ID
18/18B02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4898020..4900012 forward strand

Annotation

Disease association

MONDO:0979572 - spermatogenic failure 101

References:

GO biological process

GO:0035973 - aggrephagy

References:

GO:0000045 - autophagosome assembly

References:

GO:0016236 - macroautophagy

References:

GO:0000423 - mitophagy

References:

GO:0034727 - piecemeal microautophagy of the nucleus

References:

GO:0016485 - protein processing

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GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0000407 - phagophore assembly site

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GO molecular function

GO:0004197 - cysteine-type endopeptidase activity

References:

GO:0019786 - protein-phosphatidylethanolamide deconjugating activity

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Multi-locus phenotype

FYPO:0005256 - decreased carboxypeptidase activity

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Genotypes:

FYPO:0000668 - decreased peptidase activity

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Genotypes:

FYPO:0000539 - increased protein secretion during vegetative growth

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0004670 - abnormal macroautophagy during nitrogen starvation

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Genotypes:

FYPO:0000380 - abolished macroautophagy

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Genotypes:

FYPO:0004671 - abolished protein localization to vacuole during nitrogen starvation

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Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0000668 - decreased peptidase activity

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Genotypes:

FYPO:0002615 - decreased protein localization to pre-autophagosomal structure

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Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0002643 - normal growth on clotrimazole

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Genotypes:

FYPO:0002343 - normal growth on terbinafine

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009035 - resistance to formamide

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005969 - resistance to magnesium chloride

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0002642 - sensitive to amphotericin B

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

References:

Genotypes:

FYPO:0009069 - sensitive to ciclopirox olamine

References:

Genotypes:

FYPO:0002640 - sensitive to clotrimazole

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF03416Peptidase_C54Peptidase_C54_catPfam
SSF54001Papain-like_cys_pep_sfSUPERFAMILY
PTHR22624Peptidase_C54PANTHER

Orthologs

References / Literature

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:20070859 - Fission yeast Vps1 and Atg8 contribute to oxidative stress resistance.
Mikawa T et al. Genes Cells 2010 Mar;15(3):229-42
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:22252817 - A genomewide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis.
Fang Y et al. Antimicrob Agents Chemother 2012 Apr;56(4):1949-59
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:19778961 - Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation.
Mukaiyama H et al. Microbiology (Reading) 2009 Dec;155(Pt 12):3816-3826
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:21153812 - Processing and maturation of carboxypeptidase Y and alkaline phosphatase in Schizosaccharomyces pombe.
Mukaiyama H et al. Appl Microbiol Biotechnol 2011 Apr;90(1):203-13
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:16491466 - Construction of a protease-deficient strain set for the fission yeast Schizosaccharomyces pombe, useful for effective production of protease-sensitive heterologous proteins.
Idiris A et al. Yeast 2006 Jan 30;23(2):83-99
PMID:33064910 - Sulfur depletion induces autophagy through Ecl1 family genes in fission yeast.
Shimasaki T et al. Genes Cells 2020 Dec;25(12):825-830
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:19669754 - Enhanced protein secretion from multiprotease-deficient fission yeast by modification of its vacuolar protein sorting pathway.
Idiris A et al. Appl Microbiol Biotechnol 2010 Jan;85(3):667-77
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:26098123 - Promoter nucleosome dynamics regulated by signalling through the CTD code.
Materne P et al. Elife 2015 Jun 22;4:e09008
PMID:23231582 - Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.
Carter-O'Connell I et al. BMC Genomics 2012 Dec 12;13:697
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:16802154 - Enhanced productivity of protease-sensitive heterologous proteins by disruption of multiple protease genes in the fission yeast Schizosaccharomyces pombe.
Idiris A et al. Appl Microbiol Biotechnol 2006 Nov;73(2):404-20
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623