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protein coding gene - cid1 (SPAC19D5.03) - terminal uridylyltransferase Cid1

Gene summary

Standard name
cid1
Systematic ID
SPAC19D5.03
Product
terminal uridylyltransferase Cid1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O13833
ORFeome ID
21/21H05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5206580..5208422 forward strand

Annotation

GO biological process

GO:0060212 - negative regulation of nuclear-transcribed mRNA poly(A) tail shortening

References:

GO:0036450 - polyuridylation-dependent decapping of nuclear-transcribed mRNA

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GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

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GO molecular function

GO:0000287 - magnesium ion binding

References:

GO:1990817 - poly(A) RNA polymerase activity

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GO:0003723 - RNA binding

References:

GO:0050265 - RNA uridylyltransferase activity

References:

GO:0002134 - UTP binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0008339 - abolished 3' end uridylation of non-polyadenylated RNA

References:

Genotypes:

FYPO:0008336 - abolished mRNA poly(A) tail uridylation

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Genotypes:

FYPO:0000229 - cut

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0008332 - decreased mRNA poly(A) tail uridylation

References:

Genotypes:

FYPO:0002930 - decreased poly(A) tail length

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0008338 - increased 3' end uridylation of non-polyadenylated RNA

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0008332 - decreased mRNA poly(A) tail uridylation

References:

Genotypes:

FYPO:0005099 - decreased poly(A) polymerase activity

References:

Genotypes:

FYPO:0002927 - decreased poly(A) RNA binding

References:

Genotypes:

FYPO:0002930 - decreased poly(A) tail length

References:

Genotypes:

FYPO:0002861 - decreased poly(U) polymerase activity

References:

Genotypes:

FYPO:0005101 - decreased poly(U) RNA binding

References:

Genotypes:

FYPO:0000636 - increased cell population growth rate

References:

Genotypes:

FYPO:0006995 - normal chromatin silencing at centromere inner repeat

References:

Genotypes:

FYPO:0001522 - normal growth on caffeine

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0005100 - normal poly(U) polymerase activity

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0003668 - resistance to caffeine during cellular response to hydroxyurea

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0004586 - sensitive to caffeine and hydroxyurea

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Subunit composition

PBO:0015212 - homomeric(2)

References:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

Protein features

IDNameInterPro nameDB name
PF03828PAP_assocPAP_assocPFAM
PF22600MTPAP-like_centralMTPAP-like_centralPFAM
cd05402NT_PAP_TUTaseCDD
G3DSA:3.30.460.10:FF:000067FUNFAM
G3DSA:1.10.1410.10:FF:000018FUNFAM
SSF81631PAP/OAS1 substrate-binding domainSUPERFAMILY
SSF81301NucleotidyltransferaseNT_sfSUPERFAMILY
G3DSA:3.30.460.10Beta Polymerase, domain 2NT_sfGENE3D
G3DSA:1.10.1410.10GENE3D
PTHR12271POLY A POLYMERASE CID PAP -RELATEDPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:12218190 - Cytoplasmic poly(A) polymerases mediate cellular responses to S phase arrest.
Read RL et al. Proc Natl Acad Sci U S A 2002 Sep 17;99(19):12079-84
PMID:22608966 - Functional implications from the Cid1 poly(U) polymerase crystal structure.
Munoz-Tello P et al. Structure 2012 Jun 06;20(6):977-86
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:22751018 - Structural basis for the activity of a cytoplasmic RNA terminal uridylyl transferase.
Yates LA et al. Nat Struct Mol Biol 2012 Aug;19(8):782-787
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:10757807 - Cid1, a fission yeast protein required for S-M checkpoint control when DNA polymerase delta or epsilon is inactivated.
Wang SW et al. Mol Cell Biol 2000 May;20(9):3234-44
PMID:19430462 - Decapping is preceded by 3' uridylation in a novel pathway of bulk mRNA turnover.
Rissland OS et al. Nat Struct Mol Biol 2009 Jun;16(6):616-23
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:39333464 - Uridylation regulates mRNA decay directionality in fission yeast.
Grochowski M et al. Nat Commun 2024 Sep 27;15(1):8359
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:26098123 - Promoter nucleosome dynamics regulated by signalling through the CTD code.
Materne P et al. Elife 2015 Jun 22;4:e09008
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:10036242 - Caffeine can override the S-M checkpoint in fission yeast.
Wang SW et al. J Cell Sci 1999 Mar;112 ( Pt 6):927-37
PMID:17449726 - A family of poly(U) polymerases.
Kwak JE et al. RNA 2007 Jun;13(6):860-7
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:24322298 - A critical switch in the enzymatic properties of the Cid1 protein deciphered from its product-bound crystal structure.
Munoz-Tello P et al. Nucleic Acids Res 2014 Mar;42(5):3372-80
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:25760713 - Improved crystallization and diffraction of caffeine-induced death suppressor protein 1 (Cid1).
Yates LA et al. Acta Crystallogr F Struct Biol Commun 2015 Mar;71(Pt 3):346-53
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:25712096 - Structural plasticity of Cid1 provides a basis for its distributive RNA terminal uridylyl transferase activity.
Yates LA et al. Nucleic Acids Res 2015 Mar 11;43(5):2968-79
PMID:17512405 - RNAi-dependent and -independent RNA turnover mechanisms contribute to heterochromatic gene silencing.
Bühler M et al. Cell 2007 May 18;129(4):707-21
PMID:22139915 - The telomeric transcriptome of Schizosaccharomyces pombe.
Bah A et al. Nucleic Acids Res 2012 Apr;40(7):2995-3005
PMID:22885303 - Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity.
Lunde BM et al. Nucleic Acids Res 2012 Oct;40(19):9815-24
PMID:17353264 - Efficient RNA polyuridylation by noncanonical poly(A) polymerases.
Rissland OS et al. Mol Cell Biol 2007 May;27(10):3612-24
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55