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protein coding gene - din1 (SPAC19D5.06c) - RNA NAD-cap (NAD-forming) hydrolase/RNA pyrophosphohydrolase Din1

Gene summary

Standard name
din1
Systematic ID
SPAC19D5.06c
Product
RNA NAD-cap (NAD-forming) hydrolase/RNA pyrophosphohydrolase Din1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
rai1
UniProt ID
O13836
ORFeome ID
16/16C03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5220220..5221494 reverse strand

Annotation

GO biological process

GO:0000448 - cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

References:

GO:0110155 - NAD-cap decapping

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GO:0000956 - nuclear-transcribed mRNA catabolic process

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GO cellular component

GO:0005829 - cytosol

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GO:0090730 - Las1 complex

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GO:0005634 - nucleus

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GO molecular function

GO:0140432 - 5'-hydroxyl dinucleotide hydrolase activity

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GO:0019003 - GDP binding

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GO:0034353 - mRNA 5'-diphosphatase activity

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GO:1990174 - phosphodiesterase decapping endonuclease activity

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GO:0005515 - protein binding

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GO:0110152 - RNA NAD+-cap (NAD+-forming) hydrolase activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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Multi-locus phenotype

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0003153 - normal protein level during cellular response to heat

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000121 - abnormal sporulation

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Genotypes:

FYPO:0003664 - abolished mRNA 5'-diphosphatase activity

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0000703 - normal protein-protein interaction

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Genotypes:

FYPO:0003665 - normal RNA pyrophosphohydrolase activity

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0007808 - resistance to valproic acid

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF08652RAI1RAI1Pfam
PTHR12395RAI1-like_famPANTHER

Orthologs

References / Literature

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:11238999 - The dhp1(+) gene, encoding a putative nuclear 5'-->3' exoribonuclease, is required for proper chromosome segregation in fission yeast.
Shobuike T et al. Nucleic Acids Res 2001 Mar 15;29(6):1326-33
PMID:22912768 - Rhn1, a nuclear protein, is required for suppression of meiotic mRNAs in mitotically dividing fission yeast.
Sugiyama T et al. PLoS One 2012;7(8):e42962
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:29601584 - 2'-O-methylation of the mRNA cap protects RNAs from decapping and degradation by DXO.
Picard-Jean F et al. PLoS One 2018;13(3):e0193804
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:31777937 - A novel 5'-hydroxyl dinucleotide hydrolase activity for the DXO/Rai1 family of enzymes.
Doamekpor SK et al. Nucleic Acids Res 2020 Jan 10;48(1):349-358
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.
Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28283058 - 5' End Nicotinamide Adenine Dinucleotide Cap in Human Cells Promotes RNA Decay through DXO-Mediated deNADding.
Jiao X et al. Cell 2017 Mar 09;168(6):1015-1027.e10
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:32374864 - DXO/Rai1 enzymes remove 5'-end FAD and dephospho-CoA caps on RNAs.
Doamekpor SK et al. Nucleic Acids Res 2020 Jun 19;48(11):6136-6148
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:19194460 - Structure and function of the 5'-->3' exoribonuclease Rat1 and its activating partner Rai1.
Xiang S et al. Nature 2009 Apr 09;458(7239):784-8
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:26631744 - Conserved factor Dhp1/Rat1/Xrn2 triggers premature transcription termination and nucleates heterochromatin to promote gene silencing.
Chalamcharla VR et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15548-55
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105