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protein coding gene - mcm5 (SPAC1B2.05) - MCM complex subunit Mcm5

Gene summary

Standard name
mcm5
Systematic ID
SPAC1B2.05
Product
MCM complex subunit Mcm5
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
nda4, SPAC3F10.01
UniProt ID
P41389
ORFeome ID
28/28A08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2815661..2820694 forward strand

Annotation

Disease association

MONDO:0016817 - Meier-Gorlin syndrome

References:

MONDO:0033046 - Meier-Gorlin syndrome 8

References:

GO biological process

GO:0000727 - double-strand break repair via break-induced replication

References:

GO:1902975 - mitotic DNA replication initiation

References:

GO:0006279 - premeiotic DNA replication

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0031261 - DNA replication preinitiation complex

References:

GO:0042555 - MCM complex

References:

GO:0005656 - nuclear pre-replicative complex

References:

GO:0043596 - nuclear replication fork

References:

GO:0005634 - nucleus

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GO molecular function

GO:0043138 - 3'-5' DNA helicase activity

References:

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0003697 - single-stranded DNA binding

References:

GO:0017116 - single-stranded DNA helicase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111792 - AAA family ATPase

Qualitative gene expression

PomGeneEx:0000023 - protein level constant

References:

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000021 - protein present

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001028 - abnormal cell cycle arrest in mitotic interphase

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0001430 - abnormal mitotic cell cycle arrest with unreplicated DNA

References:

Genotypes:

FYPO:0001343 - abnormal mitotic DNA replication

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0003801 - decreased chromatin binding at replication origin

References:

Genotypes:

FYPO:0001388 - decreased protein level in germinating spore

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0001128 - decreased septation index

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000950 - elongated aseptate vegetative cell

References:

Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

References:

Genotypes:

FYPO:0001130 - increased protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0000316 - inviable after spore germination

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

References:

Genotypes:

FYPO:0001385 - inviable after spore germination, without cell division, cell cycle arrest with replicated DNA

References:

Genotypes:

FYPO:0000839 - inviable elongated mononucleate aseptate cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000012 - mitotic G2/M phase transition delay

References:

Genotypes:

FYPO:0003800 - normal chromatin binding at replication origin

References:

Genotypes:

FYPO:0001133 - normal DNA replication

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0000472 - normal mating type switching

References:

Genotypes:

FYPO:0001278 - normal MCM complex assembly

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF21933MCM5_CMCM5_CPFAM
PF17855MCM_lidMCM_lidPFAM
PF17207MCM_OBMCM_OBPFAM
PF00493MCMMCM_domPFAM
PF14551MCM_NMCM_NPFAM
cd17756MCM5CDD
PS00847MCM_1MCM_CSPROSITE_PATTERNS
PS50051MCM_2MCM_domPROSITE_PROFILES
SM00350mcmMCMSMART
PR01657MCMFAMILYMCM_domPRINTS
PR01661MCMPROTEIN5MCM5PRINTS
G3DSA:3.40.50.300:FF:000241FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
SSF50249Nucleic acid-binding proteinsNA-bd_OB-foldSUPERFAMILY
G3DSA:2.40.50.140NA-bd_OB-foldGENE3D
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
G3DSA:2.20.28.10GENE3D
G3DSA:3.30.1640.10GENE3D
PTHR11630DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBERMCMPANTHER

Orthologs

References / Literature

PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:15155581 - Chk1 activation requires Rad9 S/TQ-site phosphorylation to promote association with C-terminal BRCT domains of Rad4TOPBP1.
Furuya K et al. Genes Dev 2004 May 15;18(10):1154-64
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:12185500 - Fission yeast Cdc23 interactions with DNA replication initiation proteins.
Hart EA et al. Curr Genet 2002 Aug;41(5):342-8
PMID:10770926 - Isolation and characterization of various complexes of the minichromosome maintenance proteins of Schizosaccharomyces pombe.
Lee JK et al. J Biol Chem 2000 Jun 23;275(25):18871-8
PMID:11027263 - Schizosaccharomyces pombe Hsk1p is a potential cds1p target required for genome integrity.
Snaith HA et al. Mol Cell Biol 2000 Nov;20(21):7922-32
PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.
McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238
PMID:22036784 - Purification and functional inactivation of the fission yeast MCM(MCM-BP) complex.
Li JJ et al. FEBS Lett 2011 Dec 15;585(24):3850-5
PMID:16899242 - Analysis of Mcm2-7 chromatin binding during anaphase and in the transition to quiescence in fission yeast.
Namdar M et al. Exp Cell Res 2006 Oct 15;312(17):3360-9
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.
Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82
PMID:9914167 - Reduced dosage of a single fission yeast MCM protein causes genetic instability and S phase delay.
Liang DT et al. J Cell Sci 1999 Feb;112 ( Pt 4):559-67
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models
PMID:8838655 - Fission yeast Nda1 and Nda4, MCM homologs required for DNA replication, are constitutive nuclear proteins.
Okishio N et al. J Cell Sci 1996 Feb;109 ( Pt 2):319-26
PMID:11606526 - Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints.
Liang DT et al. Genetics 2001 Oct;159(2):471-86
GO_REF:0000051 - S. pombe keyword mapping
PMID:21813639 - Schizosaccharomyces pombe minichromosome maintenance-binding protein (MCM-BP) antagonizes MCM helicase.
Ding L et al. J Biol Chem 2011 Sep 23;286(38):32918-30
PMID:29036220 - Defective replication initiation results in locus specific chromosome breakage and a ribosomal RNA deficiency in yeast.
Sanchez JC et al. PLoS Genet 2017 Oct;13(10):e1007041
PMID:39094566 - Mrc1 regulates parental histone segregation and heterochromatin inheritance.
Toda T et al. Mol Cell 2024 Jul 23;
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.
Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:9383050 - Mutational analysis of Cdc19p, a Schizosaccharomyces pombe MCM protein.
Forsburg SL et al. Genetics 1997 Nov;147(3):1025-41
PMID:9619628 - Identification of sna41 gene, which is the suppressor of nda4 mutation and is involved in DNA replication in Schizosaccharomyces pombe.
Miyake S et al. Genes Cells 1998 Mar;3(3):157-66
GO_REF:0000002 - Comments
PMID:26670050 - Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1.
Kilchert C et al. Cell Rep 2015 Dec 22;13(11):2504-2515
PMID:9366552 - A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeast.
Adachi Y et al. Genes Cells 1997 Jul;2(7):467-79
PMID:16849602 - Regulation of DNA replication machinery by Mrc1 in fission yeast.
Nitani N et al. Genetics 2006 Sep;174(1):155-65
PMID:15194812 - A novel intermediate in initiation complex assembly for fission yeast DNA replication.
Yamada Y et al. Mol Biol Cell 2004 Aug;15(8):3740-50
PMID:10888871 - Mitotic replication initiation proteins are not required for pre-meiotic S phase.
Forsburg SL et al. Nat Genet 2000 Jul;25(3):263-8
PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.
Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80
PMID:30759238 - Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.
Singh B et al. Nucleic Acids Res 2019 Apr 23;47(7):3422-3433
PMID:9705504 - Schizosaccharomyces pombe Mcm3p, an essential nuclear protein, associates tightly with Nda4p (Mcm5p).
Sherman DA et al. Nucleic Acids Res 1998 Sep 01;26(17):3955-60
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:8298187 - Fission yeast genes nda1+ and nda4+, mutations of which lead to S-phase block, chromatin alteration and Ca2+ suppression, are members of the CDC46/MCM2 family.
Miyake S et al. Mol Biol Cell 1993 Oct;4(10):1003-15
PMID:9705352 - Purification of Hsk1, a minichromosome maintenance protein kinase from fission yeast.
Brown GW et al. J Biol Chem 1998 Aug 21;273(34):22083-90
PMID:9658174 - Multiple domains of fission yeast Cdc19p (MCM2) are required for its association with the core MCM complex.
Sherman DA et al. Mol Biol Cell 1998 Jul;9(7):1833-45
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:11973289 - Different phenotypes in vivo are associated with ATPase motif mutations in Schizosaccharomyces pombe minichromosome maintenance proteins.
Gómez EB et al. Genetics 2002 Apr;160(4):1305-18