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protein coding gene - rpb10 (SPAC1B3.12c) - DNA-directed RNA polymerase I, II, and III subunit Rpb10

Gene summary

Standard name
rpb10
Systematic ID
SPAC1B3.12c
Product
DNA-directed RNA polymerase I, II, and III subunit Rpb10
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O13877
ORFeome ID
06/06E09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4950341..4951286 reverse strand

Annotation

GO biological process

GO:0042790 - nucleolar large rRNA transcription by RNA polymerase I

References:

GO:0006363 - termination of RNA polymerase I transcription

References:

GO:0006360 - transcription by RNA polymerase I

References:

GO:0006366 - transcription by RNA polymerase II

References:

GO:0006383 - transcription by RNA polymerase III

References:

GO:0006362 - transcription elongation by RNA polymerase I

References:

GO:0006361 - transcription initiation at RNA polymerase I promoter

References:

GO:0006367 - transcription initiation at RNA polymerase II promoter

References:

GO:0042797 - tRNA transcription by RNA polymerase III

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005730 - nucleolus

References:

GO:0005634 - nucleus

References:

GO:0005736 - RNA polymerase I complex

References:

GO:0005665 - RNA polymerase II, core complex

References:

GO:0016591 - RNA polymerase II, holoenzyme

References:

GO:0005666 - RNA polymerase III complex

References:

GO molecular function

GO:0003677 - DNA binding

References:

GO:0003899 - DNA-directed RNA polymerase activity

References:

GO:0008270 - zinc ion binding

References:

Multi-locus phenotype

FYPO:0007786 - elongated cell with cell cycle arrest at meiotic G2/MI transition

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000027 - ribosomal density decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0001309 - increased viability in stationary phase

References:

Genotypes:

FYPO:0002379 - inviable after spore germination, without cell division, with elongated germ tube

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01194RNA_pol_NRPABC5/Rpb10Pfam
PS01112RNA_POL_N_8KDRNA_pol_suN_Zn-BSPROSITE patterns
G3DSA:1.10.10.60:FF:000024CATH-FunFam
G3DSA:1.10.10.60CATH-Gene3D
SSF46924RNA_pol_su_RPB10SUPERFAMILY
PTHR23431RPABC5/Rpb10PANTHER
PIRSF005653RNA_pol_N/8_subRPABC5/Rpb10PIRSF
NF003089PRK04016.1NCBIFAM
MF_00250RNApol_arch_Rpo10RPABC5/Rpb10HAMAP

Orthologs

References / Literature

PMID:33536435 - Conserved strategies of RNA polymerase I hibernation and activation.
Heiss FB et al. Nat Commun 2021 Feb 03;12(1):758
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
GO_REF:0000002 - Comments
PMID:19458260 - Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.
Spåhr H et al. Proc Natl Acad Sci U S A 2009 Jun 09;106(23):9185-90
PMID:11839823 - Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II.
Kimura M et al. Mol Cell Biol 2002 Mar;22(5):1577-88
PMID:15182371 - Mediator is required for activated transcription in a Schizosaccharomyces pombe in vitro system.
Tamayo E et al. Eur J Biochem 2004 Jun;271(12):2561-72
PMID:9190794 - [The first member of a novel family of eukaryotic transcription factors detected by the heterospecific complementation].
Shpakovskiĭ GV et al. Bioorg Khim 1997 Mar;23(3):234-7
PMID:25392932 - RNA processing factors Swd2.2 and Sen1 antagonize RNA Pol III-dependent transcription and the localization of condensin at Pol III genes.
Legros P et al. PLoS Genet 2014 Nov;10(11):e1004794
PMID:39746995 - DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts.
Kuś K et al. Nat Commun 2025 Jan 02;16(1):10
PMID:9548938 - Location of subunit-subunit contact sites on RNA polymerase II subunit 3 from the fission yeast Schizosaccharomyces pombe.
Yasui K et al. Biochemistry 1998 Apr 21;37(16):5542-8
PMID:15569151 - Hub1 is an essential ubiquitin-like protein without functioning as a typical modifier in fission yeast.
Yashiroda H et al. Genes Cells 2004 Dec;9(12):1189-97
PMID:20536828 - A screen for genes involved in respiration control and longevity in Schizosaccharomyces pombe.
Roux AE et al. Ann N Y Acad Sci 2010 Jun;1197:19-27
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:10648788 - Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II.
Kimura M et al. Nucleic Acids Res 2000 Feb 15;28(4):952-9
PMID:28476936 - Genome-wide screen for cell growth regulators in fission yeast.
Weston L et al. J Cell Sci 2017 Jun 15;130(12):2049-2055
PMID:9325316 - RNA polymerase II subunits 2, 3, and 11 form a core subassembly with DNA binding activity.
Kimura M et al. J Biol Chem 1997 Oct 10;272(41):25851-5
PMID:9157844 - [Cloning of cDNA for RNA polymerase subunit from the fission yeast Schizosaccharomyces pombe by heterospecific complementation in Saccharomyces cerevisiae].
Shpakovskiĭ GV et al. Bioorg Khim 1997 Feb;23(2):110-7
PMID:9642054 - Two large subunits of the fission yeast RNA polymerase II provide platforms for the assembly of small subunits.
Ishiguro A et al. J Mol Biol 1998 Jun 19;279(4):703-12
PMID:15743411 - Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation.
Hayashi K et al. Genes Cells 2005 Mar;10(3):207-24
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:11168400 - Intracellular contents and assembly states of all 12 subunits of the RNA polymerase II in the fission yeast Schizosaccharomyces pombe.
Kimura M et al. Eur J Biochem 2001 Feb;268(3):612-9
PMID:16204182 - RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing.
Djupedal I et al. Genes Dev 2005 Oct 01;19(19):2301-6
PMID:28241144 - Mediator structure and rearrangements required for holoenzyme formation.
Tsai KL et al. Nature 2017 Apr 13;544(7649):196-201
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:9322754 - Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II.
Sakurai H et al. Gene 1997 Sep 01;196(1-2):165-74
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:15620689 - Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit.
Mitsuzawa H et al. FEBS Lett 2005 Jan 03;579(1):48-52