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protein coding gene - csk1 (SPAC1D4.06c) - cyclin-dependent kinase activating kinase Csk1

Gene summary

Standard name
csk1
Systematic ID
SPAC1D4.06c
Product
cyclin-dependent kinase activating kinase Csk1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P36615
ORFeome ID
17/17B06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 648301..649565 reverse strand

Annotation

Complementation

PBO:0016520 - functionally complements S. cerevisiae CAK1

References:

GO biological process

GO:0030643 - intracellular phosphate ion homeostasis

References:

GO:0000122 - negative regulation of transcription by RNA polymerase II

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GO:0010971 - positive regulation of G2/M transition of mitotic cell cycle

References:

GO:0051445 - regulation of meiotic cell cycle

References:

GO:0007165 - signal transduction

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GO cellular component

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0004672 - protein kinase activity

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GO:0004674 - protein serine/threonine kinase activity

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Multi-locus phenotype

FYPO:0003172 - abnormal cell cycle arrest in mitotic M phase without septation

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Genotypes:

FYPO:0002244 - abolished acid phosphatase activity

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Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

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Genotypes:

FYPO:0005369 - abolished cell population growth at low temperature

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0001382 - decreased protein kinase activity

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Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0004416 - decreased RNA level during cellular response to phosphate starvation

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002243 - increased acid phosphatase activity

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Genotypes:

FYPO:0003128 - inviable elongated mononucleate aseptate cell with cell cycle arrest in mitotic M phase

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0000267 - sensitive to ionizing radiation during vegetative growth

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Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

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Genotypes:

FYPO:0005369 - abolished cell population growth at low temperature

References:

Genotypes:

FYPO:0002033 - abolished protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

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Genotypes:

FYPO:0009075 - decreased cell population growth on fructose carbon source

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

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Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

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Genotypes:

FYPO:0006978 - decreased cellular coenzyme Q10 level

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Genotypes:

FYPO:0000482 - decreased mitotic recombination

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Genotypes:

FYPO:0001382 - decreased protein kinase activity

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Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

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Genotypes:

FYPO:0003836 - decreased RNA polymerase II carboxy-terminal domain kinase activity

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002243 - increased acid phosphatase activity

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0003445 - increased duration of mitotic DNA damage checkpoint during cellular response to UV

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Genotypes:

FYPO:0000473 - increased mitotic recombination

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Genotypes:

FYPO:0004415 - increased phosphatase activity

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Genotypes:

FYPO:0004834 - increased phosphatase activity during cellular response to adenine starvation

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Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

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Genotypes:

FYPO:0007506 - increased protein localization to chromatin at promoter

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Genotypes:

FYPO:0006270 - increased RNA level during cellular response to phosphate starvation

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Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

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Genotypes:

FYPO:0000579 - normal spore germination

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Genotypes:

FYPO:0003200 - normal transcription regulatory region sequence-specific DNA binding during cellular response to phosphate starvation

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0009030 - resistance to amitrole

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000327 - resistance to trichostatin A

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

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Genotypes:

FYPO:0003840 - sensitive to carbendazim

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000267 - sensitive to ionizing radiation during vegetative growth

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Genotypes:

FYPO:0000107 - sensitive to latrunculin A

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000111 - sensitive to rapamycin

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Genotypes:

FYPO:0005889 - sensitive to sodium chloride

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Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

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Genotypes:

FYPO:0000112 - sensitive to sorbitol

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0002067 - slow cell population growth during recovery from stationary phase

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
PF00069PkinaseProt_kinase_domPfam
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE profiles
G3DSA:1.10.510.10:FF:001300CATH-FunFam
G3DSA:1.10.510.10CATH-Gene3D
G3DSA:3.30.200.20CATH-Gene3D
SSF56112Kinase-like_dom_sfSUPERFAMILY
PTHR24056CDKPANTHER

Orthologs

References / Literature

PMID:16428435 - Cyclin-dependent kinase 9 (Cdk9) of fission yeast is activated by the CDK-activating kinase Csk1, overlaps functionally with the TFIIH-associated kinase Mcs6, and associates with the mRNA cap methyltransferase Pcm1 in vivo.
Pei Y et al. Mol Cell Biol 2006 Feb;26(3):777-88
PMID:12084729 - Comparison of Cak1p-like cyclin-dependent kinase-activating kinases.
Tsakraklides V et al. J Biol Chem 2002 Sep 06;277(36):33482-9
PMID:10226032 - Cdc2 activation in fission yeast depends on Mcs6 and Csk1, two partially redundant Cdk-activating kinases (CAKs).
Lee KM et al. Curr Biol 1999 Apr 22;9(8):441-4
PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.
Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813
PMID:21169418 - Systematic screen of Schizosaccharomyces pombe deletion collection uncovers parallel evolution of the phosphate signal transduction pathway in yeasts.
Henry TC et al. Eukaryot Cell 2011 Feb;10(2):198-206
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:18231579 - The CDK-activating kinase (CAK) Csk1 is required for normal levels of homologous recombination and resistance to DNA damage in fission yeast.
Gerber HB et al. PLoS One 2008 Jan 30;3(1):e1492
PMID:22508988 - Separate domains of fission yeast Cdk9 (P-TEFb) are required for capping enzyme recruitment and primed (Ser7-phosphorylated) Rpb1 carboxyl-terminal domain substrate recognition.
St Amour CV et al. Mol Cell Biol 2012 Jul;32(13):2372-83
PMID:25547512 - Dissection of the PHO pathway in Schizosaccharomyces pombe using epistasis and the alternate repressor adenine.
Estill M et al. Curr Genet 2015 May;61(2):175-83
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:9857180 - Fission yeast Csk1 is a CAK-activating kinase (CAKAK).
Hermand D et al. EMBO J 1998 Dec 15;17(24):7230-8
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:25651869 - Chemical genomics approach to identify genes associated with sensitivity to rapamycin in the fission yeast Schizosaccharomyces pombe.
Doi A et al. Genes Cells 2015 Apr;20(4):292-309
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23231582 - Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.
Carter-O'Connell I et al. BMC Genomics 2012 Dec 12;13:697
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23
PMID:8467814 - Characterization of the fission yeast mcs2 cyclin and its associated protein kinase activity.
Molz L et al. EMBO J 1993 Apr;12(4):1723-32
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:11226158 - Specificity of Cdk activation in vivo by the two Caks Mcs6 and Csk1 in fission yeast.
Hermand D et al. EMBO J 2001 Jan 15;20(1-2):82-90
PMID:15829570 - Impairment of the TFIIH-associated CDK-activating kinase selectively affects cell cycle-regulated gene expression in fission yeast.
Lee KM et al. Mol Biol Cell 2005 Jun;16(6):2734-45
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:15486101 - The plant-specific kinase CDKF;1 is involved in activating phosphorylation of cyclin-dependent kinase-activating kinases in Arabidopsis.
Shimotohno A et al. Plant Cell 2004 Nov;16(11):2954-66
PMID:26264592 - RNA polymerase II CTD phospho-sites Ser5 and Ser7 govern phosphate homeostasis in fission yeast.
Schwer B et al. RNA 2015 Oct;21(10):1770-80
PMID:34967420 - Genetic screen for suppression of transcriptional interference reveals fission yeast 14-3-3 protein Rad24 as an antagonist of precocious Pol2 transcription termination.
Garg A et al. Nucleic Acids Res 2022 Jan 25;50(2):803-819
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:12121616 - A CDK-activating kinase network is required in cell cycle control and transcription in fission yeast.
Saiz JE et al. Curr Biol 2002 Jul 09;12(13):1100-5
PMID:31030285 - CoQ 10 production in Schizosaccharomyces pombe is increased by reduction of glucose levels or deletion of pka1.
Nishida I et al. Appl Microbiol Biotechnol 2019 Jun;103(12):4899-4915
PMID:30355770 - RNA polymerase II CTD interactome with 3' processing and termination factors in fission yeast and its impact on phosphate homeostasis.
Sanchez AM et al. Proc Natl Acad Sci U S A 2018 Nov 06;115(45):E10652-E10661
PMID:26098123 - Promoter nucleosome dynamics regulated by signalling through the CTD code.
Materne P et al. Elife 2015 Jun 22;4:e09008
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83