PomBase home

protein coding gene - lkh1 (SPAC1D4.11c) - dual specificity protein kinase Lkh1

Gene summary

Standard name
lkh1
Systematic ID
SPAC1D4.11c
Product
dual specificity protein kinase Lkh1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
kic1
UniProt ID
Q10156
ORFeome ID
27/27G02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 656076..660177 reverse strand

Annotation

Complementation

PBO:0016895 - functionally complemented by S. cerevisiae KNS1

References:

GO biological process

GO:0034599 - cellular response to oxidative stress

References:

GO:2000134 - negative regulation of G1/S transition of mitotic cell cycle

References:

GO:0000122 - negative regulation of transcription by RNA polymerase II

References:

GO:0048026 - positive regulation of mRNA splicing, via spliceosome

References:

GO:0000920 - septum digestion after cytokinesis

References:

GO:0023052 - signaling

References:

GO cellular component

GO:0005634 - nucleus

References:

GO molecular function

GO:0004672 - protein kinase activity

References:

GO:0004674 - protein serine/threonine kinase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00696 - phosphorylated residue

References:

Multi-locus phenotype

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0005288 - decreased negative regulation of transcription by glucose

References:

Genotypes:

FYPO:0002134 - decreased protein-RNA interaction

References:

Genotypes:

FYPO:0001035 - increased cell wall thickness during vegetative growth

References:

Genotypes:

FYPO:0001854 - increased transcription during cellular response to iron

References:

Genotypes:

FYPO:0002878 - increased transcription during glucose starvation

References:

Genotypes:

FYPO:0000780 - increased transcription during vegetative growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001404 - irregular colony morphology

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

Protein features

PBO:0111790 - CLK/STY/LAMMEr subfamily

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0000183 - abnormal poly(A)+ mRNA export from nucleus

References:

Genotypes:

FYPO:0001129 - abnormal protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0001384 - abolished protein kinase activity

References:

Genotypes:

FYPO:0002296 - cone-shaped colony morphology

References:

Genotypes:

FYPO:0003535 - decreased bipolar index

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0000709 - decreased cell population growth during nitrogen starvation

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0000603 - decreased duration of mitotic G1 phase

References:

Genotypes:

FYPO:0005288 - decreased negative regulation of transcription by glucose

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0002446 - decreased protein phosphorylation during cellular response to hydrogen peroxide

References:

Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0002134 - decreased protein-RNA interaction

References:

Genotypes:

FYPO:0001116 - decreased RNA level during cellular response to hydrogen peroxide

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0001253 - elongated multinucleate multiseptate vegetative cell, single septa between nuclei

References:

Genotypes:

FYPO:0002671 - increased concentration of protein in growth medium

References:

Genotypes:

FYPO:0001942 - increased duration of lag phase

References:

Genotypes:

FYPO:0000155 - increased flocculation

References:

Genotypes:

FYPO:0003532 - increased monopolar index

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0003776 - increased pseudohyphal growth

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001406 - increased septum thickness

References:

Genotypes:

FYPO:0001854 - increased transcription during cellular response to iron

References:

Genotypes:

FYPO:0002878 - increased transcription during glucose starvation

References:

Genotypes:

FYPO:0000780 - increased transcription during vegetative growth

References:

Genotypes:

FYPO:0001309 - increased viability in stationary phase

References:

Genotypes:

FYPO:0003619 - normal mRNA splicing, via spliceosome

References:

Genotypes:

FYPO:0000838 - normal protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0000783 - protein mislocalized to cytoplasm during vegetative growth

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0006680 - sensitive to bisphenol A

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

References:

Genotypes:

FYPO:0003840 - sensitive to carbendazim

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000107 - sensitive to latrunculin A

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000112 - sensitive to sorbitol

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

References:

Genotypes:

FYPO:0003612 - viable spore population

References:

Genotypes:

FYPO:0001491 - viable vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF00069PkinaseProt_kinase_domPFAM
cd14134PKc_CLKCDD
PS00107PROTEIN_KINASE_ATPProtein_kinase_ATP_BSPROSITE_PATTERNS
PS00108PROTEIN_KINASE_STSer/Thr_kinase_ASPROSITE_PATTERNS
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE_PROFILES
SM00220serkin_6Prot_kinase_domSMART
G3DSA:1.10.510.10:FF:000612FUNFAM
SSF56112Protein kinase-like (PK-like)Kinase-like_dom_sfSUPERFAMILY
G3DSA:3.30.200.20Phosphorylase Kinase; domain 1GENE3D
G3DSA:1.10.510.10Transferase(Phosphotransferase) domain 1GENE3D
PTHR45646SERINE/THREONINE-PROTEIN KINASE DOA-RELATEDCLK_kinasesPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder

Orthologs

References / Literature

PMID:20200159 - The LAMMER kinase homolog, Lkh1, regulates Tup transcriptional repressors through phosphorylation in Schizosaccharomyces pombe.
Kang WH et al. J Biol Chem 2010 Apr 30;285(18):13797-806
PMID:20501954 - Search for kinases related to transition of growth polarity in fission yeast.
Koyano T et al. Biosci Biotechnol Biochem 2010;74(5):1129-33
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:22098069 - Identification of a galactose-specific flocculin essential for non-sexual flocculation and filamentous growth in Schizosaccharomyces pombe.
Matsuzawa T et al. Mol Microbiol 2011 Dec;82(6):1531-44
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:12565823 - The kic1 kinase of schizosaccharomyces pombe is a CLK/STY orthologue that regulates cell-cell separation.
Tang Z et al. Exp Cell Res 2003 Feb 01;283(1):101-15
PMID:22180499 - MADS box transcription factor Mbx2/Pvg4 regulates invasive growth and flocculation by inducing gsf2+ expression in fission yeast.
Matsuzawa T et al. Eukaryot Cell 2012 Feb;11(2):151-8
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:10629038 - Biochemical and genetic conservation of fission yeast Dsk1 and human SR protein-specific kinase 1.
Tang Z et al. Mol Cell Biol 2000 Feb;20(3):816-24
PMID:21745468 - LAMMER kinase Kic1 is involved in pre-mRNA processing.
Tang Z et al. Exp Cell Res 2011 Oct 01;317(16):2308-20
PMID:11741326 - Negative regulation of filamentous growth and flocculation by Lkh1, a fission yeast LAMMER kinase homolog.
Kim KH et al. Biochem Biophys Res Commun 2001 Dec 21;289(5):1237-42
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:38180730 - LAMMER Kinase Governs the Expression and Cellular Localization of Gas2, a Key Regulator of Flocculation in Schizosaccharomyces pombe.
Kang WH et al. J Microbiol 2024 Jan 05;
PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.
Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000051 - S. pombe keyword mapping
PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.
Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:17612531 - RNA-binding protein Csx1 is phosphorylated by LAMMER kinase, Lkh1, in response to oxidative stress in Schizosaccharomyces pombe.
Kang WH et al. FEBS Lett 2007 Jul 24;581(18):3473-8
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:23376070 - Fission yeast LAMMER kinase Lkh1 regulates the cell cycle by phosphorylating the CDK-inhibitor Rum1.
Yu EY et al. Biochem Biophys Res Commun 2013 Mar 01;432(1):80-5
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:23956636 - Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis.
Cho SJ et al. Mycobiology 2010 Jun;38(2):108-12
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:14623292 - LAMMER kinase homolog, Lkh1, is involved in oxidative-stress response of fission yeast.
Park YD et al. Biochem Biophys Res Commun 2003 Nov 28;311(4):1078-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:37929004 - LAMMER Kinase Modulates Cell Cycle by Phosphorylating the MBF Repressor, Yox1, in Schizosaccharomyces pombe .
Park K et al. Mycobiology 2023;51(5):372-378
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:17362205 - Dsk1p kinase phosphorylates SR proteins and regulates their cellular localization in fission yeast.
Tang Z et al. Biochem J 2007 Jul 01;405(1):21-30
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:22683458 - Interacting factors and cellular localization of SR protein-specific kinase Dsk1.
Tang Z et al. Exp Cell Res 2012 Oct 01;318(16):2071-84
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:30294483 - LAMMER Kinase Lkh1 Is an Upstream Regulator of Prk1-Mediated Non-Sexual Flocculation in Fission Yeast.
Park YD et al. Mycobiology 2018;46(3):236-241
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87