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protein coding gene - shk2 (SPAC1F5.09c) - PAK-related kinase Shk2

Gene summary

Standard name
shk2
Systematic ID
SPAC1F5.09c
Product
PAK-related kinase Shk2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
pak2
UniProt ID
Q10056
ORFeome ID
36/36B11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 273152..274921 forward strand

Annotation

PBO:0000252 - Mkh1-Pek1-Spm1 MAP kinase

Complementation

PBO:0091259 - does not functionally complement S. cerevisiae CLA4

References:

Disease association

MONDO:0032568 - intellectual developmental disorder with macrocephaly, seizures, and speech delay

References:

MONDO:0010361 - intellectual disability, X-linked 30

References:

MONDO:0100119 - Knobloch syndrome 2

References:

MONDO:0019181 - non-syndromic X-linked intellectual disability

References:

GO biological process

GO:0009267 - cellular response to starvation

References:

GO:0032220 - plasma membrane fusion involved in cytogamy

References:

GO:1903137 - regulation of cell integrity MAPK cascade

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GO cellular component

GO:0005829 - cytosol

References:

GO:0016020 - membrane

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GO:0005739 - mitochondrion

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GO molecular function

GO:0005524 - ATP binding

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GO:0008289 - lipid binding

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GO:0005515 - protein binding

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GO:0004674 - protein serine/threonine kinase activity

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Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0003363 - abolished cytogamy

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0001122 - elongated vegetative cell

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Genotypes:

FYPO:0002150 - inviable spore population

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001886 - meiotic cell cycle entry and sporulation in haploid

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0006014 - promiscuous mating

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Genotypes:

FYPO:0000947 - swollen spherical vegetative cell

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Genotypes:

FYPO:0001492 - viable elongated vegetative cell

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Genotypes:

FYPO:0003612 - viable spore population

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Protein features

PBO:0111758 - PAK-related kinase family

PBO:0111759 - pleckstrin homology domain

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0006669 - abnormal mating between three cells

References:

Genotypes:

FYPO:0000121 - abnormal sporulation

References:

Genotypes:

FYPO:0000413 - abolished cell fusion during mating

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Genotypes:

FYPO:0006685 - abolished protein localization to mating partner nucleus in zygote

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0006673 - decreased DNA content in spore

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

References:

Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0004807 - narrow cell fusion site following mating

References:

Genotypes:

FYPO:0001147 - normal mating efficiency

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0000590 - normal sporulation

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001120 - pear-shaped vegetative cell

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Genotypes:

FYPO:0001983 - protein absent from cell

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0000073 - resistance to caffeine

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009081 - resistance to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000107 - sensitive to latrunculin A

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0006672 - transient cell fusion during mating followed by meiotic cell cycle entry and sporulation

References:

Genotypes:

FYPO:0000678 - unequal homologous chromosome segregation

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002197 - viable vegetative cell with abnormal cell shape

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00786PBDCRIB_domPFAM
PF00069PkinaseProt_kinase_domPFAM
PF00169PHPH_domainPFAM
cd13279PH_Cla4_Ste20CDD
cd01093CRIB_PAK_likePAK_BDCDD
cd06614STKc_PAKCDD
PS00107PROTEIN_KINASE_ATPProtein_kinase_ATP_BSPROSITE_PATTERNS
PS00108PROTEIN_KINASE_STSer/Thr_kinase_ASPROSITE_PATTERNS
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE_PROFILES
PS50003PH_DOMAINPH_domainPROSITE_PROFILES
PS50108CRIBCRIB_domPROSITE_PROFILES
SM00285PBD_5CRIB_domSMART
SM00220serkin_6Prot_kinase_domSMART
SM00233PH_updatePH_domainSMART
G3DSA:3.90.810.10:FF:000005FUNFAM
G3DSA:1.10.510.10:FF:000139FUNFAM
SSF50729PH domain-likeSUPERFAMILY
SSF56112Protein kinase-like (PK-like)Kinase-like_dom_sfSUPERFAMILY
G3DSA:3.90.810.10CRIB domainCRIB_dom_sfGENE3D
G3DSA:3.30.200.20Phosphorylase Kinase; domain 1GENE3D
G3DSA:2.30.29.30PH-like_dom_sfGENE3D
G3DSA:1.10.510.10Transferase(Phosphotransferase) domain 1GENE3D
PTHR45832SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATEDPAK3-likePANTHER

Orthologs

References / Literature

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:11453249 - The Schizosaccharomyces pombe Cdc42p GTPase signals through Pak2p and the Mkh1p-Pek1p-Spm1p MAP kinase pathway.
Merla A et al. Curr Genet 2001 Jun;39(4):205-9
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:9660817 - Cloning and characterization of shk2, a gene encoding a novel p21-activated protein kinase from fission yeast.
Yang P et al. J Biol Chem 1998 Jul 17;273(29):18481-9
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:14506270 - The novel Rho GTPase-activating protein family protein, Rga8, provides a potential link between Cdc42/p21-activated kinase and Rho signaling pathways in the fission yeast, Schizosaccharomyces pombe.
Yang P et al. J Biol Chem 2003 Dec 05;278(49):48821-30
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:30089908 - Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization.
Vještica A et al. Nature 2018 Aug;560(7718):397-400
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:9660818 - Characterization of Pak2p, a pleckstrin homology domain-containing, p21-activated protein kinase from fission yeast.
Sells MA et al. J Biol Chem 1998 Jul 17;273(29):18490-8
GO_REF:0000002 - Comments
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:16615890 - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.
Chikashige Y et al. Cell 2006 Apr 07;125(1):59-69
PMID:19672306 - Genome-wide screen of genes required for caffeine tolerance in fission yeast.
Calvo IA et al. PLoS One 2009 Aug 12;4(8):e6619
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PB_REF:0000003 - Disease Association Curation
PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.
Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813