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protein coding gene - pkd2 (SPAC1F7.03) - plasma membrane TRP-like calcium ion channel Pkd2

Gene summary

Standard name
pkd2
Systematic ID
SPAC1F7.03
Product
plasma membrane TRP-like calcium ion channel Pkd2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09917
ORFeome ID
37/37D12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4219751..4222702 forward strand

Annotation

Comment

PBO:0002419 - rapidly internalised

References:

GO biological process

GO:0097720 - calcineurin-mediated signaling

References:

GO:0098703 - calcium ion import across plasma membrane

References:

GO:0019722 - calcium-mediated signaling

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GO:0071474 - cellular hyperosmotic response

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GO:0009272 - fungal-type cell wall biogenesis

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GO:0009992 - intracellular water homeostasis

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GO:0090334 - regulation of cell wall (1->3)-beta-D-glucan biosynthetic process

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GO cellular component

GO:0032154 - cleavage furrow

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GO:0000935 - division septum

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GO:0005783 - endoplasmic reticulum

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GO:0000139 - Golgi membrane

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GO:0032178 - medial membrane band

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GO:0005886 - plasma membrane

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GO:0031520 - plasma membrane of cell tip

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GO molecular function

GO:0005262 - calcium channel activity

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GO:0015275 - stretch-activated, monoatomic cation-selective, calcium channel activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0006900 - decreased septum thickness

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000098 - sensitive to calcium

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007717 - sensitive to potassium nitrate

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Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

References:

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000018 - protein level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001972 - abnormal cell separation after cytokinesis resulting in septated cell

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Genotypes:

FYPO:0000806 - abnormal Golgi organization

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Genotypes:

FYPO:0000305 - abnormal spore germination

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Genotypes:

FYPO:0002196 - abnormal vegetative cell shape

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Genotypes:

FYPO:0006899 - asymmetric cell separation after cytokinesis

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Genotypes:

FYPO:0002050 - branched elongated multinucleate aseptate vegetative cell

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Genotypes:

FYPO:0006901 - cell deflation and reinflation

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Genotypes:

FYPO:0003203 - curved septum

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0001082 - decreased cell wall beta-glucan level

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Genotypes:

FYPO:0001197 - decreased cellular calcium level

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Genotypes:

FYPO:0004441 - decreased cytosolic calcium level

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Genotypes:

FYPO:0008063 - decreased immediate intracellular calcium spike following osmotic shock

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Genotypes:

FYPO:0003306 - decreased mitotic index

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Genotypes:

FYPO:0003339 - decreased rate of actomyosin contractile ring assembly

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Genotypes:

FYPO:0005628 - decreased rate of cell separation after cytokinesis

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002021 - dispersed actin cortical patch localization during vegetative growth

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Genotypes:

FYPO:0000223 - elongated multiseptate vegetative cell

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Genotypes:

FYPO:0008132 - increased calcium signaling

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Genotypes:

FYPO:0008115 - increased cell population growth on sorbitol carbon source

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Genotypes:

FYPO:0001949 - increased cytosolic calcium level

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Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

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Genotypes:

FYPO:0001130 - increased protein localization to nucleus during vegetative growth

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Genotypes:

FYPO:0006897 - increased rate of actomyosin contractile ring contraction

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Genotypes:

FYPO:0000650 - increased septation index

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Genotypes:

FYPO:0001406 - increased septum thickness

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Genotypes:

FYPO:0001460 - increased transcription from CDRE promoter in response to calcium ion

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Genotypes:

FYPO:0006211 - inviable after spore germination, before germ tube formation

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Genotypes:

FYPO:0001042 - inviable after spore germination, single or double cell division

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002150 - inviable spore population

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Genotypes:

FYPO:0001489 - inviable vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

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Genotypes:

FYPO:0001252 - multinucleate multiseptate vegetative cell

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Genotypes:

FYPO:0001368 - normal actomyosin contractile ring assembly

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Genotypes:

FYPO:0006005 - normal actomyosin contractile ring localization

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Genotypes:

FYPO:0006898 - normal cell wall thickness during vegetative growth

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Genotypes:

FYPO:0004078 - normal growth on cyclosporin A

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Genotypes:

FYPO:0005947 - normal growth on potassium chloride

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Genotypes:

FYPO:0000644 - normal protein localization during vegetative growth

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Genotypes:

FYPO:0002442 - normal protein localization to cell division site

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Genotypes:

FYPO:0000783 - protein mislocalized to cytoplasm during vegetative growth

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Genotypes:

FYPO:0002714 - protein mislocalized to Golgi apparatus

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Genotypes:

FYPO:0008114 - sensitive to amiloride

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Genotypes:

FYPO:0000094 - sensitive to benomyl

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Genotypes:

FYPO:0006680 - sensitive to bisphenol A

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Genotypes:

FYPO:0000098 - sensitive to calcium

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Genotypes:

FYPO:0001190 - sensitive to cell wall-degrading enzymes

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Genotypes:

FYPO:0000271 - sensitive to salt stress

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Genotypes:

FYPO:0000647 - vegetative cell lysis

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Genotypes:

FYPO:0001496 - viable elongated multiseptate vegetative cell

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Genotypes:

FYPO:0002106 - viable stubby vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

Protein features

IDNameInterPro nameDB name
PF06011TRPTRP_CPfam
PF14558TRP_NTRP_NPfam
SM01320TRP_NTRP_NSMART
PTHR31145TRP_Flc/Pkd2-likePANTHER
Transmembrane alpha helixDeepTMHMM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
Signal PeptideSignal-Peptide-DeepTMHMM

Orthologs

References / Literature

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:34349749 - Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect.
Moro S et al. Front Microbiol 2021;12:708354
PMID:15537393 - A microbial TRP-like polycystic-kidney-disease-related ion channel gene.
Palmer CP et al. Biochem J 2005 Apr 01;387(Pt 1):211-9
PMID:39057340 - The Mechanosensitive Pkd2 Channel Modulates the Recruitment of Myosin II and Actin to the Cytokinetic Contractile Ring.
Chowdhury P et al. J Fungi (Basel) 2024 Jun 28;10(7)
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:35099006 - Fission yeast polycystin Pkd2p promotes cell size expansion and antagonizes the Hippo-related SIN pathway.
Sinha D et al. J Cell Sci 2022 Feb 15;135(4)
PMID:36200871 - Membrane stretching activates calcium permeability of a putative channel Pkd2 during fission yeast cytokinesis.
Poddar A et al. Mol Biol Cell 2022 Dec 01;33(14):ar134
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:31116668 - Fission yeast TRP channel Pkd2p localizes to the cleavage furrow and regulates cell separation during cytokinesis.
Morris Z et al. Mol Biol Cell 2019 Jul 15;30(15):1791-1804
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:19543678 - Polycystic kidney disease channel and synaptotagmin homologues play roles in schizosaccharomyces pombe cell wall synthesis/repair and membrane protein trafficking.
Aydar E et al. J Membr Biol 2009 Jun;229(3):141-52
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:40278099 - Regulation of Yeast Cytokinesis by Calcium.
Chen Q J Fungi (Basel) 2025 Apr 02;11(4)
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:37259828 - The cytoplasmic tail of the mechanosensitive channel Pkd2 regulates its internalization and clustering in eisosomes.
Malla M et al. J Cell Sci 2023 Jun 15;136(12)
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30099677 - Genome-wide evidences of bisphenol a toxicity using Schizosaccharomyces pombe.
Kim DM et al. Arch Pharm Res 2018 Aug;41(8):830-837
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21811607 - Transient receptor potential (TRP) and Cch1-Yam8 channels play key roles in the regulation of cytoplasmic Ca2+ in fission yeast.
Ma Y et al. PLoS One 2011;6(7):e22421
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39165565 - Extracellular calcium promotes internalization and degradation of the fission yeast TRP-like calcium ion channel Pkd2.
Koyano T et al. MicroPubl Biol 2024;2024
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:37723847 - Pkd2, mutations linking to autosomal dominant polycystic kidney disease, localizes to the endoplasmic reticulum and regulates calcium signaling in fission yeast.
Koyano T et al. Genes Cells 2023 Nov;28(11):811-820