protein coding gene - rho1 (SPAC1F7.04) - Rho family GTPase Rho1

Gene summary

Standard name

rho1

Systematic ID

SPAC1F7.04

Product

Rho family GTPase Rho1

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

Q09914

ORFeome ID

32/32B05

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 4224071..4225824 forward strand

Annotation

Protein features

Orthologs

• RHOA (HGNC:667) Homo sapiens
• RHOB (HGNC:668) Homo sapiens
• RHOC (HGNC:669) Homo sapiens
• rho1 (SJAG_04666) Schizosaccharomyces japonicus
• RHO1 (YPR165W) Saccharomyces cerevisiae

References / Literature

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:16524899 - Fission yeast Rho5p GTPase is a functional paralogue of Rho1p that plays a role in survival of spores and stationary-phase cells.

Rincón SA et al. Eukaryot Cell 2006 Mar;5(3):435-46

PMID:29689193 - Mechanosensation Dynamically Coordinates Polar Growth and Cell Wall Assembly to Promote Cell Survival.

Davì V et al. Dev Cell 2018 Apr 23;45(2):170-182.e7

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PMID:20739711 - Cooperation between the septins and the actomyosin ring and role of a cell-integrity pathway during cell division in fission yeast.

Wu JQ et al. Genetics 2010 Nov;186(3):897-915

PMID:9405296 - Genes that cause aberrant cell morphology by overexpression in fission yeast: a role of a small GTP-binding protein Rho2 in cell morphogenesis.

Hirata D et al. J Cell Sci 1998 Jan;111 ( Pt 2):149-59

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:16324155 - Rho1-GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast.

Mutoh T et al. Genes Cells 2005 Dec;10(12):1189-202

PMID:27852900 - Gradients of phosphatidylserine contribute to plasma membrane charge localization and cell polarity in fission yeast.

Haupt A et al. Mol Biol Cell 2017 Jan 01;28(1):210-220

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:24498240 - Rho1 GTPase and PKC ortholog Pck1 are upstream activators of the cell integrity MAPK pathway in fission yeast.

Sánchez-Mir L et al. PLoS One 2014;9(1):e88020

PMID:18793338 - Rga2 is a Rho2 GAP that regulates morphogenesis and cell integrity in S. pombe.

Villar-Tajadura MA et al. Mol Microbiol 2008 Nov;70(4):867-81

PMID:12519200 - Rga5p is a specific Rho1p GTPase-activating protein that regulates cell integrity in Schizosaccharomyces pombe.

Calonge TM et al. Mol Microbiol 2003 Jan;47(2):507-18

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:39509469 - Rho1 and Rgf1 establish a new actin-dependent signal to determine growth poles in yeast independently of microtubules and the Tea1-Tea4 complex.

Garcia P et al. PLoS Biol 2024 Nov 07;22(11):e3002491

PMID:15546915 - Schizosaccharomyces pombe Rgf3p is a specific Rho1 GEF that regulates cell wall beta-glucan biosynthesis through the GTPase Rho1p.

Tajadura V et al. J Cell Sci 2004 Dec 01;117(Pt 25):6163-74

PMID:15537393 - A microbial TRP-like polycystic-kidney-disease-related ion channel gene.

Palmer CP et al. Biochem J 2005 Apr 01;387(Pt 1):211-9

PMID:25473118 - Regulation of Rho-GEF Rgf3 by the arrestin Art1 in fission yeast cytokinesis.

Davidson R et al. Mol Biol Cell 2015 Feb 01;26(3):453-66

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:10924454 - A new genetic method for isolating functionally interacting genes: high plo1(+)-dependent mutants and their suppressors define genes in mitotic and septation pathways in fission yeast.

Cullen CF et al. Genetics 2000 Aug;155(4):1521-34

PMID:23934882 - Negative functional interaction between cell integrity MAPK pathway and Rho1 GTPase in fission yeast.

Viana RA et al. Genetics 2013 Oct;195(2):421-32

PMID:29813053 - Specific detection of fission yeast primary septum reveals septum and cleavage furrow ingression during early anaphase independent of mitosis completion.

G Cortés JC et al. PLoS Genet 2018 May;14(5):e1007388

PMID:11737264 - Characterization of GTPase-activating proteins for the function of the Rho-family small GTPases in the fission yeast Schizosaccharomyces pombe.

Nakano K et al. Genes Cells 2001 Dec;6(12):1031-42

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:36854376 - Identification of a small RhoA GTPase inhibitor effective in fission yeast and human cells.

Morishita J et al. Open Biol 2023 Mar;13(3):220185

PMID:18256290 - Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis.

Pinar M et al. Mol Biol Cell 2008 Apr;19(4):1727-38

PMID:9491802 - The small GTP-binding protein Rho1 is a multifunctional protein that regulates actin localization, cell polarity, and septum formation in the fission yeast Schizosaccharomyces pombe.

Nakano K et al. Genes Cells 1997 Nov;2(11):679-94

PMID:34200466 - The Multiple Functions of Rho GTPases in Fission Yeasts.

Vicente-Soler J et al. Cells 2021 Jun 07;10(6)

PMID:7698654 - Isolation and sequencing of two cDNA clones encoding Rho proteins from the fission yeast Schizosaccharomyces pombe.

Nakano K et al. Gene 1995 Mar 21;155(1):119-22

PMID:19237545 - The fission yeast SEL1 domain protein Cfh3p: a novel regulator of the glucan synthase Bgs1p whose function is more relevant under stress conditions.

Sharifmoghadam MR et al. J Biol Chem 2009 Apr 24;284(17):11070-9

PMID:25651781 - Geranylgeranyltransferase Cwg2-Rho4/Rho5 module is implicated in the Pmk1 MAP kinase-mediated cell wall integrity pathway in fission yeast.

Doi A et al. Genes Cells 2015 Apr;20(4):310-23

GO_REF:0000051 - S. pombe keyword mapping

PMID:27385337 - Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis.

Liu Y et al. Mol Biol Cell 2016 Aug 15;27(16):2528-41

PMID:10504305 - Schizosaccharomyces pombe protein kinase C homologues, pck1p and pck2p, are targets of rho1p and rho2p and differentially regulate cell integrity.

Arellano M et al. J Cell Sci 1999 Oct;112 ( Pt 20):3569-78

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:11102532 - Schizosaccharomyces pombe rho2p GTPase regulates cell wall alpha-glucan biosynthesis through the protein kinase pck2p.

Calonge TM et al. Mol Biol Cell 2000 Dec;11(12):4393-401

PMID:37039135 - Cdc42 prevents precocious Rho1 activation during cytokinesis in a Pak1-dependent manner.

Onwubiko UN et al. J Cell Sci 2023 Apr 15;136(8)

PMID:30044717 - Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis.

Zhu YH et al. Mol Biol Cell 2018 Sep 15;29(19):2259-2279

PMID:14506270 - The novel Rho GTPase-activating protein family protein, Rga8, provides a potential link between Cdc42/p21-activated kinase and Rho signaling pathways in the fission yeast, Schizosaccharomyces pombe.

Yang P et al. J Biol Chem 2003 Dec 05;278(49):48821-30

PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.

UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531

PMID:41259369 - Hydroxyurea induces an oxidative stress response that triggers ER expansion and cytoplasmic protein aggregation.

Sánchez-Molina A et al. PLoS Biol 2025 Nov 19;23(11):e3003493

PMID:9781874 - Characterization of the geranylgeranyl transferase type I from Schizosaccharomyces pombe.

Arellano M et al. Mol Microbiol 1998 Sep;29(6):1357-67

PMID:9372443 - Localisation of the Schizosaccharomyces pombe rho1p GTPase and its involvement in the organisation of the actin cytoskeleton.

Arellano M et al. J Cell Sci 1997 Oct;110 ( Pt 20):2547-55

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:20519959 - S. pombe genome deletion project: an update.

Spirek M et al. Cell Cycle 2010 Jun 15;9(12):2399-402

PMID:24478458 - The checkpoint-dependent nuclear accumulation of Rho1p exchange factor Rgf1p is important for tolerance to chronic replication stress.

Muñoz S et al. Mol Biol Cell 2014 Apr;25(7):1137-50

PMID:23966468 - Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis.

Zhu YH et al. Mol Biol Cell 2013 Oct;24(20):3187-204

PMID:36358328 - Crystal Structure of Schizosaccharomyces pombe Rho1 Reveals Its Evolutionary Relationship with Other Rho GTPases.

Huang Q et al. Biology (Basel) 2022 Nov 07;11(11)

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:33386485 - Spatial sequestration of misfolded proteins as an active chaperone-mediated process during heat stress.

Boronat S et al. Curr Genet 2021 Apr;67(2):237-243

PMID:14637153 - Role of guanine nucleotide exchange factors for Rho family GTPases in the regulation of cell morphology and actin cytoskeleton in fission yeast.

Iwaki N et al. Biochem Biophys Res Commun 2003 Dec 12;312(2):414-20

PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.

Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870

PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.

Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671

PMID:23907979 - The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway.

Cruz S et al. Microbiologyopen 2013 Oct;2(5):778-94

PMID:26152728 - A Two-step Protein Quality Control Pathway for a Misfolded DJ-1 Variant in Fission Yeast.

Mathiassen SG et al. J Biol Chem 2015 Aug 21;290(34):21141-21153

PMID:16415366 - A role for the septation initiation network in septum assembly revealed by genetic analysis of sid2-250 suppressors.

Jin QW et al. Genetics 2006 Apr;172(4):2101-12

PMID:16291723 - Cell wall remodeling at the fission yeast cell division site requires the Rho-GEF Rgf3p.

Morrell-Falvey JL et al. J Cell Sci 2005 Dec 01;118(Pt 23):5563-73

PMID:19189958 - Fission yeast rgf2p is a rho1p guanine nucleotide exchange factor required for spore wall maturation and for the maintenance of cell integrity in the absence of rgf1p.

García P et al. Genetics 2009 Apr;181(4):1321-34

PMID:28536259 - Differential functional regulation of protein kinase C (PKC) orthologs in fission yeast.

Madrid M et al. J Biol Chem 2017 Jul 07;292(27):11374-11387

PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.

Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480

PB_REF:0000001 - Protein modification annotation by manual transfer of experimentally-verified annotation data to orthologs based on curator judgment of sequence features.

PMID:16146630 - Small GTPase Rho5 is a functional homologue of Rho1, which controls cell shape and septation in fission yeast.

Nakano K et al. FEBS Lett 2005 Sep 26;579(23):5181-6

PMID:19037094 - The Rho1p exchange factor Rgf1p signals upstream from the Pmk1 mitogen-activated protein kinase pathway in fission yeast.

Garcia P et al. Mol Biol Cell 2009 Jan;20(2):721-31

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:31666919 - Rho Family GTPases in Fission Yeast Cytokinesis.

Hercyk B et al. Commun Integr Biol 2019;12(1):171-180

PMID:10651902 - Rho-dependence of Schizosaccharomyces pombe Pck2.

Sayers LG et al. Genes Cells 2000 Jan;5(1):17-27

PMID:32075773 - Chaperone-Facilitated Aggregation of Thermo-Sensitive Proteins Shields Them from Degradation during Heat Stress.

Cabrera M et al. Cell Rep 2020 Feb 18;30(7):2430-2443.e4

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:8887550 - Rho 1 GTPase activates the (1-3)beta-D-glucan synthase and is involved in Schizosaccharomyces pombe morphogenesis.

Arellano M et al. EMBO J 1996 Sep 02;15(17):4584-91

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:16421249 - Rgf1p is a specific Rho1-GEF that coordinates cell polarization with cell wall biogenesis in fission yeast.

García P et al. Mol Biol Cell 2006 Apr;17(4):1620-31

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:27974503 - Traffic Through the Trans-Golgi Network and the Endosomal System Requires Collaboration Between Exomer and Clathrin Adaptors in Fission Yeast.

Hoya M et al. Genetics 2017 Feb;205(2):673-690

PMID:24336750 - Feedback regulation of SIN by Etd1 and Rho1 in fission yeast.

Alcaide-Gavilán M et al. Genetics 2014 Feb;196(2):455-70

PMID:37446379 - Formation of Transient Protein Aggregate-like Centers Is a General Strategy Postponing Degradation of Misfolded Intermediates.

Boronat S et al. Int J Mol Sci 2023 Jul 07;24(13)

PMID:19714215 - The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.

Aligianni S et al. PLoS Genet 2009 Aug;5(8):e1000626