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protein coding gene - hri1 (SPAC20G4.03c) - eIF2 alpha kinase Hri1

Gene summary

Standard name
hri1
Systematic ID
SPAC20G4.03c
Product
eIF2 alpha kinase Hri1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O13889
ORFeome ID
28/28A04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4818012..4820651 reverse strand

Annotation

Comment

PBO:0000206 - deletion mutant expression profiling

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Disease association

MONDO:0030513 - dystonia 33

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MONDO:0030035 - leukoencephalopathy, developmental delay, and episodic neurologic regression syndrome

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MONDO:0009192 - Wolcott-Rallison syndrome

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GO biological process

GO:1990625 - negative regulation of cytoplasmic translational initiation in response to stress

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GO:0023052 - signaling

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GO cellular component

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0004694 - eukaryotic translation initiation factor 2alpha kinase activity

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GO:0005515 - protein binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00048 - O4'-phospho-L-tyrosine

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Multi-locus phenotype

FYPO:0002130 - abolished protein phosphorylation during cellular response to heat

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Genotypes:

FYPO:0002350 - normal stress granule assembly during vegetative growth

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Genotypes:

Protein features

PBO:0111807 - HRI subfamily

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000021 - protein present

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0003743 - decreased cell population growth during glucose starvation

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Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0004295 - multiseptate cell

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Genotypes:

FYPO:0001236 - normal growth on cycloheximide

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Genotypes:

FYPO:0007559 - normal growth on diethylstilbestrol

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Genotypes:

FYPO:0007558 - normal growth on econazole

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Genotypes:

FYPO:0000962 - normal growth on hydrogen peroxide

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Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

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Genotypes:

FYPO:0000979 - normal growth on miconazole

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Genotypes:

FYPO:0002343 - normal growth on terbinafine

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Genotypes:

FYPO:0007557 - normal growth on tolnaftate

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Genotypes:

FYPO:0000385 - normal macroautophagy

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0009060 - resistance to wortmannin

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0003840 - sensitive to carbendazim

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Genotypes:

FYPO:0007555 - sensitive to econazole

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Genotypes:

FYPO:0002167 - sensitive to ethyl methanesulfonate

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000107 - sensitive to latrunculin A

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Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00069PkinaseProt_kinase_domPFAM
PF22949HRI2_3HHRI2_3HPFAM
cd13996STKc_EIF2AKCDD
PS00107PROTEIN_KINASE_ATPProtein_kinase_ATP_BSPROSITE_PATTERNS
PS00108PROTEIN_KINASE_STSer/Thr_kinase_ASPROSITE_PATTERNS
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE_PROFILES
SM00220serkin_6Prot_kinase_domSMART
SSF56112Protein kinase-like (PK-like)Kinase-like_dom_sfSUPERFAMILY
G3DSA:3.30.200.20Phosphorylase Kinase; domain 1GENE3D
G3DSA:1.10.510.10Transferase(Phosphotransferase) domain 1GENE3D
PTHR11042EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATEDCC_SR_KinasePANTHER
CoilCoilCOILS

Orthologs

References / Literature

PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72
PMID:23687372 - New roles of the fission yeast eIF2α kinases Hri1 and Gcn2 in response to nutritional stress.
Martín R et al. J Cell Sci 2013 Jul 15;126(Pt 14):3010-20
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:20547592 - Vgl1, a multi-KH domain protein, is a novel component of the fission yeast stress granules required for cell survival under thermal stress.
Wen WL et al. Nucleic Acids Res 2010 Oct;38(19):6555-66
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:31657618 - A Genome-Wide Screen for Wortmannin-Resistant Mutants in Schizosaccharomyces pombe: The Phosphorylation-Impaired Mutants Are Resistant to Signaling Defect.
Yılmazer M et al. DNA Cell Biol 2019 Dec;38(12):1427-1436
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:18502752 - Int6/eIF3e promotes general translation and Atf1 abundance to modulate Sty1 MAPK-dependent stress response in fission yeast.
Udagawa T et al. J Biol Chem 2008 Aug 08;283(32):22063-75
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:19880757 - Role of mitogen-activated protein kinase Sty1 in regulation of eukaryotic initiation factor 2alpha kinases in response to environmental stress in Schizosaccharomyces pombe.
Berlanga JJ et al. Eukaryot Cell 2010 Jan;9(1):194-207
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:33534698 - Tripartite suppression of fission yeast TORC1 signaling by the GATOR1-Sea3 complex, the TSC complex, and Gcn2 kinase.
Fukuda T et al. Elife 2021 Feb 03;10
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:22840777 - Analyzing fission yeast multidrug resistance mechanisms to develop a genetically tractable model system for chemical biology.
Kawashima SA et al. Chem Biol 2012 Jul 27;19(7):893-901
PMID:12242291 - Phosphorylation of eukaryotic initiation factor 2 by heme-regulated inhibitor kinase-related protein kinases in Schizosaccharomyces pombe is important for fesistance to environmental stresses.
Zhan K et al. Mol Cell Biol 2002 Oct;22(20):7134-46
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:33223513 - Systematic Target Screening Revealed That Tif302 Could Be an Off-Target of the Antifungal Terbinafine in Fission Yeast.
Lee S et al. Biomol Ther (Seoul) 2021 Mar 01;29(2):234-247
PMID:16278445 - Stress-activated protein kinase pathway functions to support protein synthesis and translational adaptation in response to environmental stress in fission yeast.
Dunand-Sauthier I et al. Eukaryot Cell 2005 Nov;4(11):1785-93
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.
Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7