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protein coding gene - tfs1 (SPAC20H4.03c) - general transcription elongation factor TFIIS

Gene summary

Standard name
tfs1
Systematic ID
SPAC20H4.03c
Product
general transcription elongation factor TFIIS
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P49373
ORFeome ID
50/50E02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2114913..2116456 reverse strand

Annotation

GO biological process

GO:0006368 - transcription elongation by RNA polymerase II

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0003711 - transcription elongation factor activity

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GO:0008270 - zinc ion binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0006993 - decreased chromatin silencing at centromere otr1R

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Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0003096 - decreased histone H3-K9 methylation at centromere outer repeat during vegetative growth

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Genotypes:

FYPO:0000220 - increased centromeric outer repeat transcript level

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Genotypes:

FYPO:0001740 - increased gross chromosomal rearrangement

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Genotypes:

FYPO:0001742 - increased isochromosome formation

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Genotypes:

FYPO:0001840 - increased minichromosome loss during vegetative growth

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Genotypes:

FYPO:0007227 - increased number of R-loops at centromere

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Genotypes:

FYPO:0003009 - increased protein localization to centromere outer repeat

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Genotypes:

FYPO:0004237 - increased protein localization to heterochromatin at centromere outer repeat region

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Genotypes:

FYPO:0005978 - increased spliced cen-dg RNA level

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Genotypes:

FYPO:0003049 - increased transcriptional readthrough

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Genotypes:

FYPO:0003176 - normal meiotic chromosome segregation

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Genotypes:

FYPO:0001513 - normal mitotic sister chromatid segregation

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Genotypes:

FYPO:0000069 - resistance to thiabendazole

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Genotypes:

FYPO:0000084 - sensitive to 6-azauracil

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Genotypes:

FYPO:0000098 - sensitive to calcium

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Genotypes:

FYPO:0000107 - sensitive to latrunculin A

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

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Genotypes:

Protein features

PBO:0111854 - zf-C2C2 type

PBO:0111743 - zinc finger protein

Qualitative gene expression

PomGeneEx:0000020 - protein level unchanged

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0007741 - abnormal protein distribution along RNA polymerase II-transcribed genes during mitotic metaphase

References:

Genotypes:

FYPO:0004065 - abnormal transcription elongation from RNA polymerase II promoter

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Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

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Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0002894 - decreased transcription during cellular response to calcium ion

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Genotypes:

FYPO:0009007 - decreased vegetative cell population viability

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Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

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Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0009016 - increased cell population viability on xylose carbon source

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Genotypes:

FYPO:0001840 - increased minichromosome loss during vegetative growth

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0004344 - increased viability upon nitrogen starvation

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0006992 - normal chromatin silencing at centromere otr1R

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Genotypes:

FYPO:0006811 - normal gross chromosomal rearrangement frequency

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Genotypes:

FYPO:0000964 - normal growth on thiabendazole

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Genotypes:

FYPO:0002620 - normal growth on trichostatin A

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Genotypes:

FYPO:0002389 - normal protein localization to heterochromatin at centromere outer repeat

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Genotypes:

FYPO:0002896 - normal transcription during cellular response to calcium ion

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Genotypes:

FYPO:0001855 - normal transcription during vegetative growth

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Genotypes:

FYPO:0001315 - normal vegetative cell morphology

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Genotypes:

FYPO:0007736 - protein displaced from 3' end to 5' end of RNA polymerase II-transcribed genes during mitotic metaphase

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0009036 - resistance to benzamidine

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0009045 - resistance to calcofluor and potassium chloride

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Genotypes:

FYPO:0001029 - resistance to canavanine

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Genotypes:

FYPO:0001450 - resistance to cold

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002767 - resistance to terbinafine

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000069 - resistance to thiabendazole

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Genotypes:

FYPO:0000327 - resistance to trichostatin A

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0007808 - resistance to valproic acid

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0000084 - sensitive to 6-azauracil

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

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Genotypes:

FYPO:0009069 - sensitive to ciclopirox olamine

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Genotypes:

FYPO:0001245 - sensitive to cobalt

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0007932 - sensitive to hydroxyurea and methyl methanesulfonate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0002617 - sensitive to sodium butyrate

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Genotypes:

FYPO:0005889 - sensitive to sodium chloride

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0000925 - unequal meiotic chromosome segregation

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01096Zn_ribbon_TFIISZnf_TFIISPfam
PF07500TFIIS_MTFIIS_cen_domPfam
PF08711Med26TFIIS_NPfam
cd00183TFIIS_ICDD
cd13749Zn-ribbon_TFIISCDD
PS00466ZF_TFIIS_1Znf_TFIISPROSITE patterns
PS51133ZF_TFIIS_2Znf_TFIISPROSITE profiles
PS51319TFIIS_NTFIIS_NPROSITE profiles
PS51321TFIIS_CENTRALTFIIS_cen_domPROSITE profiles
SM00440ZnF_C2C2Znf_TFIISSMART
SM00509TFS2NTFIIS/CRSP70_N_subSMART
SM00510TFS2MTFIIS_cen_domSMART
G3DSA:1.10.472.30:FF:000003CATH-FunFam
G3DSA:1.20.930.10:FF:000007CATH-FunFam
G3DSA:2.20.25.10:FF:000001CATH-FunFam
G3DSA:1.10.472.30TFIIS_cen_dom_sfCATH-Gene3D
G3DSA:1.20.930.10TFIIS/LEDGF_dom_sfCATH-Gene3D
G3DSA:2.20.25.10CATH-Gene3D
SSF46942TFIIS_cen_dom_sfSUPERFAMILY
SSF47676TFIIS/LEDGF_dom_sfSUPERFAMILY
SSF57783SUPERFAMILY
PTHR11477PANTHER
PIRSF006704TF_IISTF_IIS-typPIRSF
TIGR01385TFSIITFSIINCBIFAM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity

Orthologs

References / Literature

GO_REF:0000002 - Comments
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30652128 - Heterochromatin suppresses gross chromosomal rearrangements at centromeres by repressing Tfs1/TFIIS-dependent transcription.
Okita AK et al. Commun Biol 2019;2:17
PMID:41525994 - Transcriptional PBR cycles at pericentromeric repeats cause gross chromosomal rearrangements through Rad52-dependent ADR-loop formation.
Xu R et al. Nucleic Acids Res 2026 Jan 05;54(1)
PMID:41526646 - Development of a global screening system for detecting protein-protein interactions by luminescence complementation in fission yeast.
Azadeh F et al. Sci Rep 2026 Jan 12;
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26173815 - Genome-wide screen of fission yeast mutants for sensitivity to 6-azauracil, an inhibitor of transcriptional elongation.
Zhou H et al. Yeast 2015 Oct;32(10):643-55
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:8904334 - Isolation and characterization of the Schizosaccharomyces pombe gene encoding transcript elongation factor TFIIS.
Williams LA et al. Yeast 1996 Mar 15;12(3):227-36
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23861937 - Genome-wide screening for genes associated with valproic acid sensitivity in fission yeast.
Zhang L et al. PLoS One 2013;8(7):e68738
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:25240800 - The RNA exosome promotes transcription termination of backtracked RNA polymerase II.
Lemay JF et al. Nat Struct Mol Biol 2014 Oct;21(10):919-26
PMID:34984977 - Functional profiling of long intergenic non-coding RNAs in fission yeast.
Rodriguez-Lopez M et al. Elife 2022 Jan 05;11
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:33771877 - RNA polymerase backtracking results in the accumulation of fission yeast condensin at active genes.
Rivosecchi J et al. Life Sci Alliance 2021 Jun;4(6)
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:24945319 - CPF-associated phosphatase activity opposes condensin-mediated chromosome condensation.
Vanoosthuyse V et al. PLoS Genet 2014 Jun;10(6):e1004415
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:37052630 - Tfs1, transcription elongation factor TFIIS, has an impact on chromosome segregation affected by pka1 deletion in Schizosaccharomyces pombe.
Takenaka K et al. Curr Genet 2023 Jun;69(2-3):115-125
PMID:10648612 - The Rpb6 subunit of fission yeast RNA polymerase II is a contact target of the transcription elongation factor TFIIS.
Ishiguro A et al. Mol Cell Biol 2000 Feb;20(4):1263-70
PMID:41984257 - pka1 deletion induces hyperactivation of the transcription factor Mca1 and drives chromosome mis-segregation in Schizosaccharomyces pombe.
Yamawaki M et al. Mol Genet Genomics 2026 Apr 15;301(1)
PMID:21892171 - Defects in RNA quality control factors reveal RNAi-independent nucleation of heterochromatin.
Reyes-Turcu FE et al. Nat Struct Mol Biol 2011 Sep 04;18(10):1132-8
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:26631744 - Conserved factor Dhp1/Rat1/Xrn2 triggers premature transcription termination and nucleates heterochromatin to promote gene silencing.
Chalamcharla VR et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15548-55
PMID:17614284 - The chromatin-remodeling factor FACT contributes to centromeric heterochromatin independently of RNAi.
Lejeune E et al. Curr Biol 2007 Jul 17;17(14):1219-24
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:41316862 - Deletion of Elongator Protein 1 (Elp1) relieves heterochromatin defects in a Pol II mutant of Schizosaccharomyces pombe.
Nirmal MB et al. Genetics 2025 Nov 29;
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:22496451 - Genetic screening for regulators of Prz1, a transcriptional factor acting downstream of calcineurin in fission yeast.
Koike A et al. J Biol Chem 2012 Jun 01;287(23):19294-303
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105