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protein coding gene - fml2 (SPAC20H4.04) - ATP-dependent 3' to 5' DNA helicase

Gene summary

Standard name
fml2
Systematic ID
SPAC20H4.04
Product
ATP-dependent 3' to 5' DNA helicase
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
mfh2
UniProt ID
Q9HE09
ORFeome ID
49/49E05
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome I: 2116525..2120279 forward strand

Annotation

Disease association

MONDO:0054862 - premature ovarian failure 15

References:

MONDO:0054732 - spermatogenic failure 28

References:

GO biological process

GO:0045003 - double-strand break repair via synthesis-dependent strand annealing

References:

GO:0036297 - interstrand cross-link repair

References:

GO:1903461 - Okazaki fragment processing involved in mitotic DNA replication

References:

GO cellular component

GO:0005730 - nucleolus

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0043138 - 3'-5' DNA helicase activity

References:

GO:0005524 - ATP binding

References:

GO:0000400 - four-way junction DNA binding

References:

GO:0009378 - four-way junction helicase activity

References:

GO:0016787 - hydrolase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

Multi-locus phenotype

FYPO:0004159 - abnormal homologous chromosome segregation

References:

Genotypes:

FYPO:0006419 - increased duration of Rad51 focus presence during meiotic cell cycle

References:

Genotypes:

Protein features

PBO:0111747 - DEAD/DEAH box helicase

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000121 - abnormal sporulation

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF04851ResIIIHelicase/UvrB_NPFAM
PF00271Helicase_CHelicase_C-likePFAM
cd18801SF2_C_FANCM_HefCDD
cd18033DEXDc_FANCMFANCM_DEXDcCDD
cd12091FANCM_IDFANCM/Mph1-like_IDCDD
PS51194HELICASE_CTERHelicase_C-likePROSITE_PROFILES
PS51192HELICASE_ATP_BIND_1Helicase_ATP-bdPROSITE_PROFILES
SM00490helicmild6Helicase_C-likeSMART
SM00487ultradead3Helicase_ATP-bdSMART
G3DSA:3.40.50.300:FF:000861FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
PTHR14025FANCONI ANEMIA GROUP M FANCM FAMILY MEMBERPANTHER

Orthologs

References / Literature

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.
Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
GO_REF:0000002 - Comments
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:22723423 - The fission yeast FANCM ortholog directs non-crossover recombination during meiosis.
Lorenz A et al. Science 2012 Jun 22;336(6088):1585-8
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.
Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:27304859 - Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper Meiotic Chromosome Segregation.
Polakova S et al. PLoS Genet 2016 Jun;12(6):e1006102