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protein coding gene - hri2 (SPAC222.07c) - eIF2 alpha kinase Hri2

Gene summary

Standard name
hri2
Systematic ID
SPAC222.07c
Product
eIF2 alpha kinase Hri2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9UTE5
ORFeome ID
37/37D05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 954279..956610 reverse strand

Annotation

Comment

PBO:0000206 - deletion mutant expression profiling

References:

Disease association

MONDO:0030513 - dystonia 33

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MONDO:0030035 - leukoencephalopathy, developmental delay, and episodic neurologic regression syndrome

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MONDO:0009192 - Wolcott-Rallison syndrome

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GO biological process

GO:0070314 - G1 to G0 transition

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GO:1990625 - negative regulation of cytoplasmic translational initiation in response to stress

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GO:0023052 - signaling

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GO cellular component

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0004694 - eukaryotic translation initiation factor 2alpha kinase activity

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GO:0005515 - protein binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0006232 - abolished protein phosphorylation during cellular response to hydrogen peroxide

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Genotypes:

FYPO:0002033 - abolished protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0001838 - decreased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0000962 - normal growth on hydrogen peroxide

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

Protein features

PBO:0111807 - HRI subfamily

Qualitative gene expression

PomGeneEx:0000021 - protein present

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PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0006012 - elongated cell with abolished shmoo formation

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0001908 - increased pre-mRNA level

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0003295 - normal growth on amitrole

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Genotypes:

FYPO:0000385 - normal macroautophagy

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Genotypes:

FYPO:0000776 - normal protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009060 - resistance to wortmannin

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0002167 - sensitive to ethyl methanesulfonate

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF22949HRI2_3HHRI2_3HPFAM
PF00069PkinaseProt_kinase_domPFAM
cd13996STKc_EIF2AKCDD
PS00108PROTEIN_KINASE_STSer/Thr_kinase_ASPROSITE_PATTERNS
PS00107PROTEIN_KINASE_ATPProtein_kinase_ATP_BSPROSITE_PATTERNS
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE_PROFILES
SM00220serkin_6Prot_kinase_domSMART
SSF56112Protein kinase-like (PK-like)Kinase-like_dom_sfSUPERFAMILY
G3DSA:3.30.200.20Phosphorylase Kinase; domain 1GENE3D
G3DSA:1.10.510.10Transferase(Phosphotransferase) domain 1GENE3D
PTHR11042EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATEDCC_SR_KinasePANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity

Orthologs

References / Literature

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.
Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:12242291 - Phosphorylation of eukaryotic initiation factor 2 by heme-regulated inhibitor kinase-related protein kinases in Schizosaccharomyces pombe is important for fesistance to environmental stresses.
Zhan K et al. Mol Cell Biol 2002 Oct;22(20):7134-46
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:16278445 - Stress-activated protein kinase pathway functions to support protein synthesis and translational adaptation in response to environmental stress in fission yeast.
Dunand-Sauthier I et al. Eukaryot Cell 2005 Nov;4(11):1785-93
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:18502752 - Int6/eIF3e promotes general translation and Atf1 abundance to modulate Sty1 MAPK-dependent stress response in fission yeast.
Udagawa T et al. J Biol Chem 2008 Aug 08;283(32):22063-75
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23671279 - Receptor for activated C-kinase (RACK1) homolog Cpc2 facilitates the general amino acid control response through Gcn2 kinase in fission yeast.
Tarumoto Y et al. J Biol Chem 2013 Jun 28;288(26):19260-8
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:27172183 - Interconnections Between RNA-Processing Pathways Revealed by a Sequencing-Based Genetic Screen for Pre-mRNA Splicing Mutants in Fission Yeast.
Larson A et al. G3 (Bethesda) 2016 Jun 01;6(6):1513-23
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:33534698 - Tripartite suppression of fission yeast TORC1 signaling by the GATOR1-Sea3 complex, the TSC complex, and Gcn2 kinase.
Fukuda T et al. Elife 2021 Feb 03;10
PMID:23687372 - New roles of the fission yeast eIF2α kinases Hri1 and Gcn2 in response to nutritional stress.
Martín R et al. J Cell Sci 2013 Jul 15;126(Pt 14):3010-20
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:30236788 - Cmk2 kinase is essential for survival in arsenite by modulating translation together with RACK1 orthologue Cpc2 in Schizosaccharomyces pombe.
Sanchez-Marinas M et al. Free Radic Biol Med 2018 Dec;129:116-126
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:31657618 - A Genome-Wide Screen for Wortmannin-Resistant Mutants in Schizosaccharomyces pombe: The Phosphorylation-Impaired Mutants Are Resistant to Signaling Defect.
Yılmazer M et al. DNA Cell Biol 2019 Dec;38(12):1427-1436
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12