PomBase home

protein coding gene - byr4 (SPAC222.10c) - SIN signalling inhibitor, two-component GAP Byr4

Gene summary

Standard name
byr4
Systematic ID
SPAC222.10c
Product
SIN signalling inhibitor, two-component GAP Byr4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q10951
ORFeome ID
37/37B08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 962314..964808 reverse strand

Annotation

GO biological process

GO:0001100 - negative regulation of exit from mitosis

References:

GO:1902413 - negative regulation of mitotic cytokinesis

References:

GO:0031030 - negative regulation of septation initiation signaling

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0035974 - meiotic spindle pole body

References:

GO:0044732 - mitotic spindle pole body

References:

GO:0071957 - old mitotic spindle pole body

References:

GO:0160065 - SIN/MEN signaling complex

References:

GO:1990334 - SIN/MEN two-component GAP complex

References:

GO molecular function

GO:0005096 - GTPase activator activity

References:

GO:0005515 - protein binding

References:

GO:0035591 - signaling adaptor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0004481 - abolished cell population growth at high temperature

References:

Genotypes:

FYPO:0000272 - abolished septum assembly

References:

Genotypes:

FYPO:0006023 - elongated multinucleate multiseptate vegetative cell, irregular septum position

References:

Genotypes:

FYPO:0000133 - elongated multinucleate vegetative cell

References:

Genotypes:

FYPO:0004355 - increased protein phosphorylation during mitosis

References:

Genotypes:

FYPO:0004082 - normal protein phosphorylation during mitosis

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0003245 - telophase nuclear clustering

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000013 - RNA level unchanged

References:

PomGeneEx:0000014 - RNA present

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000619 - abnormal cell cycle arrest in mitotic anaphase

References:

Genotypes:

FYPO:0003830 - abnormal cytokinesis checkpoint

References:

Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

References:

Genotypes:

FYPO:0003542 - abolished protein localization to meiotic spindle pole body

References:

Genotypes:

FYPO:0002033 - abolished protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0000272 - abolished septum assembly

References:

Genotypes:

FYPO:0004562 - binucleate aseptate vegetative cell

References:

Genotypes:

FYPO:0005055 - binucleate multiseptate cell, septa grouped

References:

Genotypes:

FYPO:0001222 - binucleate vegetative cell

References:

Genotypes:

FYPO:0003440 - cell lysis during cytokinesis

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0001875 - decreased asymmetric protein localization, with protein localized to both mitotic spindle pole bodies

References:

Genotypes:

FYPO:0001876 - decreased asymmetric protein localization, with protein localized to both mitotic spindle pole bodies during anaphase

References:

Genotypes:

FYPO:0000940 - decreased protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0000133 - elongated multinucleate vegetative cell

References:

Genotypes:

FYPO:0002970 - increased protein localization to mitotic spindle pole body during metaphase

References:

Genotypes:

FYPO:0000316 - inviable after spore germination

References:

Genotypes:

FYPO:0002515 - inviable after spore germination, without cell division, with branched, elongated germ tube

References:

Genotypes:

FYPO:0003005 - inviable after spore germination, without cell division, with swollen, branched, elongated germ tube

References:

Genotypes:

FYPO:0002024 - inviable elongated multinucleate aseptate vegetative cell

References:

Genotypes:

FYPO:0004657 - inviable mononucleate multiseptate vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000284 - large and small daughter nuclei, with unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0001904 - premature actomyosin contractile ring disassembly

References:

Genotypes:

FYPO:0007256 - premature protein localization to meiotic spindle pole body during prophase I

References:

Genotypes:

FYPO:0007646 - septated mononucleate vegetative cell with mislocalized nucleus and anucleate compartment

References:

Genotypes:

FYPO:0000280 - sterile

References:

Genotypes:

FYPO:0003245 - telophase nuclear clustering

References:

Genotypes:

FYPO:0000647 - vegetative cell lysis

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF30667Byr4Bfa1/Byr4Pfam
PTHR35140Bfa1/Byr4PANTHER
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMobiDB-Negative-Polyelectrolyte
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:11271422 - Bypass of the requirement for cdc16p GAP function in Schizosaccharomyces pombe by mutation of the septation initiation network genes.
Fournier N et al. Arch Microbiol 2001 Jan;175(1):62-9
PMID:12546793 - Mitotic hyperphosphorylation of the fission yeast SIN scaffold protein cdc11p is regulated by the protein kinase cdc7p.
Krapp A et al. Curr Biol 2003 Jan 21;13(2):168-72
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:10805785 - Sid4p is required to localize components of the septation initiation pathway to the spindle pole body in fission yeast.
Chang L et al. Proc Natl Acad Sci U S A 2000 May 09;97(10):5249-54
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:10381387 - Asymmetry of the spindle pole bodies and spg1p GAP segregation during mitosis in fission yeast.
Cerutti L et al. J Cell Sci 1999 Jul;112 ( Pt 14):2313-21
PMID:10196225 - Regions of Byr4, a regulator of septation in fission yeast, that bind Spg1 or Cdc16 and form a two-component GTPase-activating protein with Cdc16.
Furge KA et al. J Biol Chem 1999 Apr 16;274(16):11339-43
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:18252797 - Homoeostasis between the GTPase Spg1p and its GAP in the regulation of cytokinesis in S. pombe.
Krapp A et al. J Cell Sci 2008 Mar 01;121(Pt 5):601-8
PMID:21131906 - Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase.
Johnson AE et al. EMBO J 2011 Jan 19;30(2):341-54
PMID:22119525 - SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.
Singh NS et al. Curr Biol 2011 Dec 06;21(23):1968-78
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:10799520 - Byr4 localizes to spindle-pole bodies in a cell cycle-regulated manner to control Cdc7 localization and septation in fission yeast.
Li C et al. J Biol Chem 2000 May 12;275(19):14381-7
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:8682866 - A novel suppressor of ras1 in fission yeast, byr4, is a dosage-dependent inhibitor of cytokinesis.
Song K et al. J Cell Biol 1996 Jun;133(6):1307-19
PMID:11514436 - Correct regulation of the septation initiation network in Schizosaccharomyces pombe requires the activities of par1 and par2.
Jiang W et al. Genetics 2001 Aug;158(4):1413-29
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:10924454 - A new genetic method for isolating functionally interacting genes: high plo1(+)-dependent mutants and their suppressors define genes in mitotic and septation pathways in fission yeast.
Cullen CF et al. Genetics 2000 Aug;155(4):1521-34
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:9638658 - Byr4, a dosage-dependent regulator of cytokinesis in S. pombe, interacts with a possible small GTPase pathway including Spg1 and Cdc16.
Jwa M et al. Mol Cells 1998 Apr 30;8(2):240-5
PMID:9742395 - Byr4 and Cdc16 form a two-component GTPase-activating protein for the Spg1 GTPase that controls septation in fission yeast.
Furge KA et al. Curr Biol 1998 Aug 27;8(17):947-54
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
GO_REF:0000002 - Comments
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24838944 - Dma1-dependent degradation of SIN proteins during meiosis in Schizosaccharomyces pombe.
Krapp A et al. J Cell Sci 2014 Jul 15;127(Pt 14):3149-61
PMID:15132994 - Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity.
Grallert A et al. Genes Dev 2004 May 01;18(9):1007-21
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:10769201 - The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation.
Salimova E et al. J Cell Sci 2000 May;113 ( Pt 10):1695-704
PMID:11676915 - S. pombe cdc11p, together with sid4p, provides an anchor for septation initiation network proteins on the spindle pole body.
Krapp A et al. Curr Biol 2001 Oct 16;11(20):1559-68
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:24055157 - CK1 is required for a mitotic checkpoint that delays cytokinesis.
Johnson AE et al. Curr Biol 2013 Oct 07;23(19):1920-6
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:24920823 - Cdk1 promotes cytokinesis in fission yeast through activation of the septation initiation network.
Rachfall N et al. Mol Biol Cell 2014 Aug 01;25(15):2250-9
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85