protein coding gene - rna4 (SPAC227.12) - U4/U6 x U5 tri-snRNP complex WD repeat subunit Rna4

Gene summary

Standard name: rna4
Systematic ID: SPAC227.12
Product: U4/U6 x U5 tri-snRNP complex WD repeat subunit Rna4
Organism: Schizosaccharomyces pombe (fission yeast)
Synonyms: prp4
UniProt ID: Q9UTC7
ORFeome ID: 23/23D05
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome I: 515794..517504 forward strand

Annotation

Disease association

MONDO:0014400 - retinitis pigmentosa 70

References:

PB_REF:0000006

GO biological process

GO:0045292 - mRNA cis splicing, via spliceosome

References:

PMID:21386897

GO cellular component

GO:0005829 - cytosol

References:

PMID:16823372

GO:0046540 - U4/U6 x U5 tri-snRNP complex

References:

PMID:21386897

GO molecular function

GO:0030621 - U4 snRNA binding

References:

GO_REF:0000033

GO:0017070 - U6 snRNA binding

References:

GO_REF:0000033

Protein features

PBO:0111751 - WD repeat protein

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PMID:39367033

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

PMID:23697806

Genotypes:

rna4delta

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

PMID:23697806

Genotypes:

rna4delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

rna4delta

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF08799	PRP4	PRP4-like	PFAM
PF00400	WD40	WD40_rpt	PFAM
cd00200	WD40		CDD
PS00678	WD_REPEATS_1	WD40_repeat_CS	PROSITE_PATTERNS
PS50294	WD_REPEATS_REGION		PROSITE_PROFILES
PS50082	WD_REPEATS_2	WD40_rpt	PROSITE_PROFILES
SM00500	pr04_2	PRP4-like	SMART
SM00320	WD40_4	WD40_rpt	SMART
PR00320	GPROTEINBRPT	WD40_PAC1	PRINTS
G3DSA:2.130.10.10:FF:000411			FUNFAM
G3DSA:4.10.280.110:FF:000015			FUNFAM
SSF158230	PRP4-like	PRP4-like_sf	SUPERFAMILY
SSF50978	WD40 repeat-like	WD40_repeat_dom_sf	SUPERFAMILY
G3DSA:4.10.280.110		PRP4-like_sf	GENE3D
G3DSA:2.130.10.10		WD40/YVTN_repeat-like_dom_sf	GENE3D
PTHR19846	WD40 REPEAT PROTEIN		PANTHER

Orthologs

PRPF4 (HGNC:17349) Homo sapiens
rna4 (SJAG_00531) Schizosaccharomyces japonicus
PRP4 (YPR178W) Saccharomyces cerevisiae

References / Literature

PMID:18948543 - Splicing factors facilitate RNAi-directed silencing in fission yeast.: Bayne EH et al. Science 2008 Oct 24;322(5901):602-6
PMID:15755920 - Dim1p is required for efficient splicing and export of mRNA encoding lid1p, a component of the fission yeast anaphase-promoting complex.: Carnahan RH et al. Eukaryot Cell 2005 Mar;4(3):577-87
PMID:21386897 - Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.: Ren L et al. PLoS One 2011 Feb 28;6(2):e16719
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:28446597 - The histone variant H2A.Z promotes splicing of weak introns.: Nissen KE et al. Genes Dev 2017 Apr 01;31(7):688-701
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.: Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000033 - Annotation inferences using phylogenetic trees