PomBase home

protein coding gene - isu1 (SPAC227.13c) - mitochondrial [2Fe-2S] cluster assembly protein Isu1

Gene summary

Standard name
isu1
Systematic ID
SPAC227.13c
Product
mitochondrial [2Fe-2S] cluster assembly protein Isu1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9UTC6
ORFeome ID
50/50A08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 517416..518951 reverse strand

Annotation

Disease association

MONDO:0009706 - hereditary myopathy with lactic acidosis due to ISCU deficiency

References:

MONDO:0009637 - inborn mitochondrial myopathy

References:

GO biological process

GO:0044571 - [2Fe-2S] cluster assembly

References:

GO:0006879 - intracellular iron ion homeostasis

References:

GO:0016226 - iron-sulfur cluster assembly

References:

GO cellular component

GO:0099128 - mitochondrial [2Fe-2S] assembly complex

References:

GO:0005759 - mitochondrial matrix

References:

GO:0005739 - mitochondrion

References:

GO molecular function

GO:0051537 - 2 iron, 2 sulfur cluster binding

References:

GO:0008198 - ferrous iron binding

References:

GO:0051536 - iron-sulfur cluster binding

References:

GO:0140132 - iron-sulfur cluster chaperone activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00739 - iron-sulfur cluster containing modification

References:

MOD:01148 - ubiquitinylated lysine

References:

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

References:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

References:

Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

References:

Genotypes:

FYPO:0001736 - decreased iron-sulfur cluster transfer

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000077 - resistance to rapamycin

References:

Genotypes:

FYPO:0000327 - resistance to trichostatin A

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0009067 - sensitive to amorolfine

References:

Genotypes:

FYPO:0006680 - sensitive to bisphenol A

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0001710 - stabilized iron-sulfur cluster binding

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Subunit composition

PBO:0091268 - homomeric(1)

References:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01592NifU_NNIF_FeS_clus_asmbl_NifU_NPfam
cd06664IscU_likeNIF_FeS_clus_asmbl_NifU_NCDD
G3DSA:3.90.1010.10:FF:000005CATH-FunFam
G3DSA:3.90.1010.10CATH-Gene3D
SSF82649SUPERFAMILY
PTHR10093NIF_FeS_clus_asmbl_NifU_NPANTHER
TIGR01999iscUISCUNCBIFAM

Orthologs

References / Literature

GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:41974504 - Nuclear basket subunits Nup211 and Rsm1 influence RNA 3'-processing and transcription termination in fission yeast.
Bednor L et al. RNA 2026 Apr 13;
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:12102630 - Iron-sulfur cluster biosynthesis. Kinetic analysis of [2Fe-2S] cluster transfer from holo ISU to apo Fd: role of redox chemistry and a conserved aspartate.
Wu SP et al. Biochemistry 2002 Jul 16;41(28):8876-85
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:17477863 - Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study.
Rustici G et al. Genome Biol 2007;8(5):R73
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:35657410 - Loss of PPR protein Ppr2 induces ferroptosis-like cell death in Schizosaccharomyces pombe.
Liu Z et al. Arch Microbiol 2022 Jun 03;204(7):360
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:11939799 - Characterization of an iron-sulfur cluster assembly protein (ISU1) from Schizosaccharomyces pombe.
Wu G et al. Biochemistry 2002 Apr 16;41(15):5024-32
PMID:20085751 - Monothiol glutaredoxin Grx5 interacts with Fe-S scaffold proteins Isa1 and Isa2 and supports Fe-S assembly and DNA integrity in mitochondria of fission yeast.
Kim KD et al. Biochem Biophys Res Commun 2010 Feb 12;392(3):467-72
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:34660592 - Biogenesis of Iron-Sulfur Clusters and Their Role in DNA Metabolism.
Shi R et al. Front Cell Dev Biol 2021;9:735678
PMID:29079705 - A novel de novo dominant mutation in ISCU associated with mitochondrial myopathy.
Legati A et al. J Med Genet 2017 Dec;54(12):815-824
PMID:26545917 - Iron-Sulfur Cluster Biogenesis Chaperones: Evidence for Emergence of Mutational Robustness of a Highly Specific Protein-Protein Interaction.
Delewski W et al. Mol Biol Evol 2016 Mar;33(3):643-56
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24997422 - The role of frataxin in fission yeast iron metabolism: implications for Friedreich's ataxia.
Wang Y et al. Biochim Biophys Acta 2014 Oct;1840(10):3022-33
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323