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protein coding gene - crd1 (SPAC22A12.08c) - cardiolipin synthase/ hydrolase fusion protein Crd1 OR mitochondrial hydrolase, N-terminal portion of Crd1 fusion protein

Gene summary

Standard name
crd1
Systematic ID
SPAC22A12.08c
Product
cardiolipin synthase/ hydrolase fusion protein Crd1 OR mitochondrial hydrolase, N-terminal portion of Crd1 fusion protein
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O13899
ORFeome ID
46/46B08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1169936..1172209 reverse strand

Annotation

Comment

PBO:0091300 - retention of intron IV leads to production of tandem hydrolase/cardiolipin fusion protein

References:

PBO:0091303 - splicing of all introns, including intron IV, introduces stop codon that leads to production of mitochondrial hydrolase instead of tandem hydrolase/cardiolipin fusion protein

References:

PBO:0091301 - transcript retaining intron IV represents 15% of total mRNA

References:

PBO:0091304 - transcript with all introns spliced represents 85% of total mRNA

References:

Complementation

PBO:0091299 - functionally complemented by S. cerevisiae CRD1

References:

PBO:0091298 - functionally complements S. cerevisiae CRD1

References:

Disease association

MONDO:0859337 - combined oxidative phosphorylation deficiency 57

References:

GO biological process

GO:0032049 - cardiolipin biosynthetic process

References:

GO:0007006 - mitochondrial membrane organization

References:

GO:0055091 - phospholipid homeostasis

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GO cellular component

GO:0005743 - mitochondrial inner membrane

References:

GO:0005739 - mitochondrion

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GO molecular function

GO:0043337 - cardiolipin synthase (CMP-forming) activity

References:

GO:0008808 - cardiolipin synthase activity

References:

GO:0016787 - hydrolase activity

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Genome organisation

PBO:0002475 - alternative splicing

References:

PBO:0000228 - domain combination not observed in other organisms

PBO:0000229 - tandem fusion protein

Modification

MOD:00046 - O-phospho-L-serine

References:

Protein sequence feature

SO:0100011 - cleaved_peptide_region

References:

SO:0001808 - mitochondrial_targeting_signal

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001422 - decreased protein processing during vegetative growth

References:

Genotypes:

FYPO:0002111 - inviable tapered vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF01066CDP-OH_P_transfCDP-OH_P_transPfam
PF13242Hydrolase_likePfam
PF13344Hydrolase_6HAD-SF_hydro_IIAPfam
cd07511HAD_likeCDD
PS00379CDP_ALCOHOL_P_TRANSFCDP_ALCOHOL_P_TRANSF_CSPROSITE patterns
G3DSA:1.20.120.1760CDP-OH_PTrfase_TM_domCATH-Gene3D
G3DSA:3.40.50.1000HAD_sfCATH-Gene3D
SSF56784HAD-like_sfSUPERFAMILY
PTHR14269CDP-alcohol_PTase-IPANTHER
TIGR01456CECR5HAD-SF_hydro_IIA_CECR5NCBIFAM
TIGR01460HAD-SF-IIAHAD-SF_hydro_IIANCBIFAM
Transmembrane alpha helixDeepTMHMM

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:29958934 - Schizosaccharomyces pombe cardiolipin synthase is part of a mitochondrial fusion protein regulated by intron retention.
Virčíková V et al. Biochim Biophys Acta Mol Cell Biol Lipids 2018 Oct;1863(10):1331-1344
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:34706246 - Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns.
Hümmer S et al. Cell Rep 2021 Oct 26;37(4):109893
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs