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protein coding gene - gmh3 (SPAC22E12.06c) - alpha-1,2-galactosyltransferase Gmh3

Gene summary

Standard name
gmh3
Systematic ID
SPAC22E12.06c
Product
alpha-1,2-galactosyltransferase Gmh3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q10359
ORFeome ID
14/14F03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5027605..5030413 reverse strand

Annotation

GO biological process

GO:0006487 - protein N-linked glycosylation

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GO cellular component

GO:0000139 - Golgi membrane

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GO molecular function

GO:0031278 - alpha-1,2-galactosyltransferase activity

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Modification

MOD:00006 - N-glycosylated residue

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Multi-locus phenotype

FYPO:0006277 - abnormal N-linked glycoprotein glycan structure

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Genotypes:

FYPO:0006278 - abnormal O-linked glycoprotein glycan structure

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Genotypes:

FYPO:0003354 - decreased galactose level in glycoprotein glycan

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Genotypes:

FYPO:0003447 - decreased level of glycosylated protein in cell

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0003291 - galactose absent from glycoprotein glycan

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0001120 - pear-shaped vegetative cell

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Genotypes:

FYPO:0000106 - sensitive to hygromycin B

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

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Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0006807 - decreased galactose level in cell surface glycoprotein glycan

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Genotypes:

FYPO:0003354 - decreased galactose level in glycoprotein glycan

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Genotypes:

FYPO:0006806 - decreased galactosyltransferase activity

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Genotypes:

FYPO:0003447 - decreased level of glycosylated protein in cell

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0000073 - resistance to caffeine

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009035 - resistance to formamide

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0005193 - resistance to torin1

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
PF05637Glyco_transf_34Glyco_trans_34PFAM
G3DSA:3.90.550.10:FF:000149FUNFAM
G3DSA:3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain ANucleotide-diphossugar_transGENE3D
PTHR31306ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATEDGlyco_trans_34PANTHER

Orthologs

References / Literature

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:28821619 - Dsc E3 ligase localization to the Golgi requires the ATPase Cdc48 and cofactor Ufd1 for activation of sterol regulatory element-binding protein in fission yeast.
Burr R et al. J Biol Chem 2017 Sep 29;292(39):16333-16350
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:33909078 - Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
Fukunaga T et al. Glycobiology 2021 Sep 09;31(8):1037-1045
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:9839953 - Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe.
Yoko-o T et al. Eur J Biochem 1998 Nov 01;257(3):630-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:22988247 - Identification of novel α1,3-galactosyltransferase and elimination of α-galactose-containing glycans by disruption of multiple α-galactosyltransferase genes in Schizosaccharomyces pombe.
Ohashi T et al. J Biol Chem 2012 Nov 09;287(46):38866-75
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:21098516 - Structural analysis of α1,3-linked galactose-containing oligosaccharides in Schizosaccharomyces pombe mutants harboring single and multiple α-galactosyltransferase genes disruptions.
Ohashi T et al. Glycobiology 2011 Mar;21(3):340-51