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protein coding gene - krp1 (SPAC22E12.09c) - kexin, type I membrane endopeptidase

Gene summary

Standard name
krp1
Systematic ID
SPAC22E12.09c
Product
kexin, type I membrane endopeptidase
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
krp
UniProt ID
Q09175
ORFeome ID
33/33F09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5034187..5036580 reverse strand

Annotation

Complementation

PBO:0008071 - functionally complemented by S. cerevisiae KEX2

References:

PBO:0008072 - functionally complemented by S. cerevisiae YPS1

References:

Disease association

MONDO:0010961 - obesity due to prohormone convertase I deficiency

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GO biological process

GO:0000747 - conjugation with cellular fusion

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GO:0016485 - protein processing

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GO cellular component

GO:0009986 - cell surface

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GO:0005794 - Golgi apparatus

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GO:0000139 - Golgi membrane

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GO:0016020 - membrane

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GO:0005802 - trans-Golgi network

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GO molecular function

GO:0004252 - serine-type endopeptidase activity

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Modification

MOD:00689 - disulfide crosslinked residues

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MOD:00006 - N-glycosylated residue

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MOD:00160 - N4-glycosyl-L-asparagine

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MOD:00396 - O-glycosylated residue

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Protein sequence feature

SO:0100011 - cleaved_peptide_region

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SO:0000418 - signal_peptide

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SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000027 - ribosomal density decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000182 - abnormal cell wall organization during vegetative growth

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Genotypes:

FYPO:0000669 - abolished peptidase activity

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Genotypes:

FYPO:0001669 - abolished protein processing during vegetative growth

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0000668 - decreased peptidase activity

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Genotypes:

FYPO:0001422 - decreased protein processing during vegetative growth

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0000316 - inviable after spore germination

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Genotypes:

FYPO:0003333 - inviable lemon-shaped cell

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Genotypes:

FYPO:0002187 - inviable normal volume spheroid vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000224 - lemon-shaped cell

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Genotypes:

FYPO:0001387 - loss of viability at high temperature

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Genotypes:

FYPO:0000665 - normal peptidase activity

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Genotypes:

FYPO:0001668 - normal protein processing during vegetative growth

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Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

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Genotypes:

FYPO:0000646 - swollen vegetative cell

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00082Peptidase_S8Peptidase_S8/S53_domPfam
PF01483P_proproteinP_domPfam
cd04059Peptidases_S8_Protein_convertases_Kexins_Furin-likeKexin/furinCDD
PS00136SUBTILASE_ASPPeptidase_S8_Asp-ASPROSITE patterns
PS00137SUBTILASE_HISPeptidase_S8_His-ASPROSITE patterns
PS00138SUBTILASE_SERPeptidase_S8_Ser-ASPROSITE patterns
PS51829P_HOMO_BP_domPROSITE profiles
PS51892SUBTILASEPROSITE profiles
PR00723SUBTILISINPeptidase_S8_subtilisin-relPRINTS
G3DSA:2.60.120.260:FF:000026CATH-FunFam
G3DSA:3.40.50.200:FF:000005CATH-FunFam
G3DSA:2.60.120.260CATH-Gene3D
G3DSA:3.40.50.200Peptidase_S8/S53_dom_sfCATH-Gene3D
SSF49785Galactose-bd-like_sfSUPERFAMILY
SSF52743Peptidase_S8/S53_dom_sfSUPERFAMILY
PTHR42884PANTHER
Transmembrane alpha helixDeepTMHMM
Signal PeptideDeepTMHMM-Signal-Peptide

Orthologs

References / Literature

PMID:10931354 - A temperature-sensitive Krp1 allows in vivo characterization of kexin activation.
Ladds G et al. Mol Microbiol 2000 Aug;37(3):606-18
PMID:11115118 - Identification of proteases with shared functions to the proprotein processing protease Krp1 in the fission yeast Schizosaccharomyces pombe.
Ladds G et al. Mol Microbiol 2000 Nov;38(4):839-53
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:7813430 - Isolation and characterization of krp, a dibasic endopeptidase required for cell viability in the fission yeast Schizosaccharomyces pombe.
Davey J et al. EMBO J 1994 Dec 15;13(24):5910-21
PMID:19624755 - Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeast.
Ma Y et al. Genes Cells 2009 Aug;14(8):1015-28
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:8654749 - Characterisation of Krp1, an endopeptidase that is essential for cell viability in fission yeast.
Powner D et al. Biochem Soc Trans 1995 Nov;23(4):564S
PMID:9418887 - Activation of the kexin from Schizosaccharomyces pombe requires internal cleavage of its initially cleaved prosequence.
Powner D et al. Mol Cell Biol 1998 Jan;18(1):400-8
PMID:9388669 - Kexin activation requires both the removal and the destruction of the pro-sequence.
Powner D et al. Biochem Soc Trans 1997 Aug;25(3):445S
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:12132578 - Cloning and characterization of the kinesin-related protein, Krp1p, in Schizosaccharomyces pombe.
Jeong JW et al. Mol Cells 2002 Jun 30;13(3):389-98
PMID:8736869 - Maturation of Krp1, an endopeptidase from the fission yeast Schizosaccharomyces pombe.
Powner D et al. Biochem Soc Trans 1996 May;24(2):211S
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000051 - S. pombe keyword mapping
PMID:8879047 - Maturation of Krp1, an endopeptidase from the fission yeast Schizosaccharomyces pombe.
Powner D et al. Biochem Soc Trans 1996 Aug;24(3):503S
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:16491466 - Construction of a protease-deficient strain set for the fission yeast Schizosaccharomyces pombe, useful for effective production of protease-sensitive heterologous proteins.
Idiris A et al. Yeast 2006 Jan 30;23(2):83-99