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protein coding gene - etp1 (SPAC22E12.10c) - mitochondrial [2Fe-2S] cluster assembly ferredoxin Etp1/ heme O monooxygenase Cox15, fusion protein

Gene summary

Standard name
etp1
Systematic ID
SPAC22E12.10c
Product
mitochondrial [2Fe-2S] cluster assembly ferredoxin Etp1/ heme O monooxygenase Cox15, fusion protein
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
cox15
UniProt ID
Q10361
ORFeome ID
26/26E08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5036954..5039152 reverse strand

Annotation

Disease association

MONDO:0014051 - cardioencephalomyopathy, fatal infantile, due to cytochrome c oxidase deficiency 2

References:

MONDO:0015487 - fatal infantile encephalocardiomyopathy

References:

MONDO:0020714 - mitochondrial myopathy, episodic, with optic atrophy and reversible leukoencephalopathy

References:

GO biological process

GO:0044571 - [2Fe-2S] cluster assembly

References:

GO:0006784 - heme A biosynthetic process

References:

GO:0140647 - P450-containing electron transport chain

References:

GO:0006744 - ubiquinone biosynthetic process

References:

GO cellular component

GO:0099128 - mitochondrial [2Fe-2S] assembly complex

References:

GO:0005743 - mitochondrial inner membrane

References:

GO:0005759 - mitochondrial matrix

References:

GO:0005739 - mitochondrion

References:

GO molecular function

GO:0051537 - 2 iron, 2 sulfur cluster binding

References:

GO:0009055 - electron transfer activity

References:

GO:0120547 - heme A synthase activity

References:

GO:0051536 - iron-sulfur cluster binding

References:

GO:0016651 - oxidoreductase activity, acting on NAD(P)H

References:

GO:0016653 - oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor

References:

Genome organisation

PBO:0000228 - domain combination not observed in other organisms

PBO:0000229 - tandem fusion protein

Modification

MOD:00739 - iron-sulfur cluster containing modification

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Protein sequence feature

SO:0100011 - cleaved_peptide_region

References:

SO:0001808 - mitochondrial_targeting_signal

References:

SO:0001812 - transmembrane_helix

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0008145 - decreased level of heme O in cell

References:

Genotypes:

FYPO:0008143 - heme A absent from cell

References:

Genotypes:

FYPO:0008144 - heme O absent from cell

References:

Genotypes:

FYPO:0000310 - inviable after spore germination, without cell division, with normal germ tube morphology

References:

Genotypes:

FYPO:0002482 - inviable spheroid vegetative cell

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011067 - conserved in bacteria

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Warnings

PBO:0000070 - gene structure updated

References:

PBO:0034747 - post-translationally cleaved tandem fusion

Protein features

IDNameInterPro nameDB name
PF00111Fer22Fe-2S_ferredoxin-typePfam
PF02628COX15-CtaACOX15/CtaA_famPfam
cd00207fer22Fe-2S_ferredoxin-typeCDD
PS00814ADXAdrenodoxin_Fe-S_BSPROSITE patterns
PR00355ADRENODOXINAdrenodoxin-likePRINTS
G3DSA:3.10.20.30Beta-grasp_dom_sfCATH-Gene3D
SSF542922Fe-2S_ferredoxin-like_sfSUPERFAMILY
PTHR23289HemeA_synthase_2PANTHER
Transmembrane alpha helixDeepTMHMM
MF_01665HemeA_synth_type2HemeA_synthase_2HAMAP

Orthologs

References / Literature

PMID:21497579 - Redox chemistry of the Schizosaccharomyces pombe ferredoxin electron-transfer domain and influence of Cys to Ser substitutions.
Wu SP et al. J Inorg Biochem 2011 Jun;105(6):806-11
PMID:12102630 - Iron-sulfur cluster biosynthesis. Kinetic analysis of [2Fe-2S] cluster transfer from holo ISU to apo Fd: role of redox chemistry and a conserved aspartate.
Wu SP et al. Biochemistry 2002 Jul 16;41(28):8876-85
PMID:15219990 - The adrenodoxin-like ferredoxin of Schizosaccharomyces pombe mitochondria.
Schiffler B et al. J Inorg Biochem 2004 Jul;98(7):1229-37
PMID:34660592 - Biogenesis of Iron-Sulfur Clusters and Their Role in DNA Metabolism.
Shi R et al. Front Cell Dev Biol 2021;9:735678
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:12741836 - Iron-sulfur cluster biosynthesis. A comparative kinetic analysis of native and Cys-substituted ISA-mediated [2Fe-2S]2+ cluster transfer to an apoferredoxin target.
Wu SP et al. Biochemistry 2003 May 20;42(19):5784-91
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:18399988 - The endogenous adrenodoxin reductase-like flavoprotein arh1 supports heterologous cytochrome P450-dependent substrate conversions in Schizosaccharomyces pombe.
Ewen KM et al. FEMS Yeast Res 2008 May;8(3):432-41
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:11939799 - Characterization of an iron-sulfur cluster assembly protein (ISU1) from Schizosaccharomyces pombe.
Wu G et al. Biochemistry 2002 Apr 16;41(15):5024-32
PMID:11941510 - Iron-sulfur cluster biosynthesis: characterization of Schizosaccharomyces pombe Isa1.
Wu G et al. J Biol Inorg Chem 2002 Apr;7(4-5):526-32
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PB_REF:0000003 - Disease Association Curation
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:11248251 - Involvement of mitochondrial ferredoxin and Cox15p in hydroxylation of heme O.
Barros MH et al. FEBS Lett 2001 Mar 09;492(1-2):133-8
PMID:21536008 - Structural and thermodynamic characterization of the adrenodoxin-like domain of the electron-transfer protein Etp1 from Schizosaccharomyces pombe.
Müller JJ et al. J Inorg Biochem 2011 Jul;105(7):957-65
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:11841224 - Functional expression of human mitochondrial CYP11B2 in fission yeast and identification of a new internal electron transfer protein, etp1.
Bureik M et al. Biochemistry 2002 Feb 19;41(7):2311-21
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000002 - Comments
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:18488015 - Dynamic repertoire of a eukaryotic transcriptome surveyed at single-nucleotide resolution.
Wilhelm BT et al. Nature 2008 Jun 26;453(7199):1239-43
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.