PomBase home

protein coding gene - pik1 (SPAC22E12.16c) - Golgi-specific 1-phosphatidylinositol 4-kinase, type III-beta Pik1

Gene summary

Standard name
pik1
Systematic ID
SPAC22E12.16c
Product
Golgi-specific 1-phosphatidylinositol 4-kinase, type III-beta Pik1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q10366
ORFeome ID
29/29F03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5049155..5052408 reverse strand

Annotation

Disease association

MONDO:0859525 - hearing loss, autosomal dominant 87

References:

GO biological process

GO:0046854 - phosphatidylinositol phosphate biosynthetic process

References:

GO:0048015 - phosphatidylinositol-mediated signaling

References:

GO:0000281 - mitotic cytokinesis

References:

GO cellular component

GO:0005794 - Golgi apparatus

References:

GO:0005634 - nucleus

References:

GO:0032588 - trans-Golgi network membrane

References:

GO:0032153 - cell division site

References:

GO molecular function

GO:0004430 - 1-phosphatidylinositol 4-kinase activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002150 - inviable spore population

References:

Genotypes:

FYPO:0004467 - normal protein localization to cell tip during cellular response to salt stress

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000027 - ribosomal density decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0006116 - abolished protein localization to actin cortical patch

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0006775 - decreased cellular phosphatidylinositol-4-phosphate level

References:

Genotypes:

FYPO:0006628 - decreased phosphatidylinositol-4-phosphate level in plasma membrane

References:

Genotypes:

FYPO:0008133 - decreased phosphatidylinositol-4-phosphate level in the Golgi

References:

Genotypes:

FYPO:0005736 - decreased prospore membrane formation

References:

Genotypes:

FYPO:0007489 - decreased protein localization to Golgi apparatus, with protein mislocalized to cytosol

References:

Genotypes:

FYPO:0008040 - increased cellular phosphatidylinositol-4,5-bisphosphate level

References:

Genotypes:

FYPO:0008330 - increased phosphatidylinositol-4-phosphate level in plasma membrane

References:

Genotypes:

FYPO:0008375 - increased phosphatidylinositol-4-phosphate level in the Golgi

References:

Genotypes:

FYPO:0002127 - increased protein localization to plasma membrane during vegetative growth

References:

Genotypes:

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001367 - normal cytokinesis

References:

Genotypes:

FYPO:0003736 - normal mitotic index

References:

Genotypes:

FYPO:0000644 - normal protein localization during vegetative growth

References:

Genotypes:

FYPO:0000744 - normal protein localization to actin cortical patch

References:

Genotypes:

FYPO:0004467 - normal protein localization to cell tip during cellular response to salt stress

References:

Genotypes:

FYPO:0002674 - normal protein localization to plasma membrane

References:

Genotypes:

FYPO:0001903 - normal septation index

References:

Genotypes:

FYPO:0002253 - normal septum location

References:

Genotypes:

FYPO:0006777 - prospore membrane formation excluding nucleus

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0000024 - stubby vegetative cell

References:

Genotypes:

FYPO:0000646 - swollen vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0006616 - viable vegetative cell with increased cell diameter

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00454PI3_PI4_kinasePI3/4_kinase_cat_domPfam
PF11522Pik1Pik1_hairpinPfam
PF21245PI4KB-PIK1_PIKPI4KB-PIK1_PIKPfam
cd05168PI4Kc_III_betaPI4-kinase_beta/PIK1_catCDD
PS00915PI3_4_KINASE_1PI3/4_kinase_CSPROSITE patterns
PS00916PI3_4_KINASE_2PI3/4_kinase_CSPROSITE patterns
PS50290PI3_4_KINASE_3PI3/4_kinase_cat_domPROSITE profiles
PS51545PIK_HELICALPI3K_accessory_domPROSITE profiles
SM00146PI3KcPI3/4_kinase_cat_domSMART
G3DSA:1.10.1070.11:FF:000016CATH-FunFam
G3DSA:3.30.1010.10:FF:000021CATH-FunFam
G3DSA:1.10.1070.11PI3/4_kinase_cat_sfCATH-Gene3D
G3DSA:3.30.1010.10CATH-Gene3D
G3DSA:6.10.140.1260CATH-Gene3D
SSF48371ARM-type_foldSUPERFAMILY
SSF56112Kinase-like_dom_sfSUPERFAMILY
PTHR10048PI3/4_kinasePANTHER
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMobiDB-Negative-Polyelectrolyte

Orthologs

References / Literature

PMID:20123972 - Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast.
Onishi M et al. Mol Cell Biol 2010 Apr;30(8):2057-74
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:21693583 - A calmodulin-related light chain from fission yeast that functions with myosin-I and PI 4-kinase.
Sammons MR et al. J Cell Sci 2011 Jul 15;124(Pt 14):2466-77
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:11087749 - Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe, interacts with a phosphatidylinositol 4-kinase.
Desautels M et al. J Biol Chem 2001 Feb 23;276(8):5932-42
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:32918581 - Golgi localization of glycosyltransferases requires Gpp74p in Schizosaccharomyces pombe.
Ohashi T et al. Appl Microbiol Biotechnol 2020 Oct;104(20):8897-8909
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:39540318 - Pck2 association with the plasma membrane and efficient response of the cell integrity pathway require regulation of PI4P homeostasis by exomer.
Moscoso-Romero E et al. Open Biol 2024 Nov;14(11):240101
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:29410177 - Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1.
Sugihara A et al. Biochem Biophys Res Commun 2018 Feb 19;496(4):1284-1290
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:21288895 - Structure of a Ca2+-myristoyl switch protein that controls activation of a phosphatidylinositol 4-kinase in fission yeast.
Lim S et al. J Biol Chem 2011 Apr 08;286(14):12565-77
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23503588 - The exoribonuclease Dis3L2 defines a novel eukaryotic RNA degradation pathway.
Malecki M et al. EMBO J 2013 Jul 03;32(13):1842-54
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:37815455 - Characterization of Pik1 function in fission yeast reveals its conserved role in lipid synthesis and not cytokinesis.
Willet AH et al. J Cell Sci 2023 Nov 01;136(21)
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895