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protein coding gene - gcs1 (SPAC22F3.10c) - glutamate-cysteine ligase Gcs1

Gene summary

Standard name
gcs1
Systematic ID
SPAC22F3.10c
Product
glutamate-cysteine ligase Gcs1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
apd1, gsh1
UniProt ID
Q09768
ORFeome ID
28/28B03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 683834..686347 forward strand

Annotation

Disease association

MONDO:0009259 - gamma-glutamylcysteine synthetase deficiency

References:

MONDO:0012039 - myocardial infarction, susceptibility to

References:

GO biological process

GO:0098849 - cellular detoxification of cadmium ion

References:

GO:0071276 - cellular response to cadmium ion

References:

GO:0006750 - glutathione biosynthetic process

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0017109 - glutamate-cysteine ligase complex

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0004357 - glutamate-cysteine ligase activity

References:

Modification

MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001570 - decreased cellular glutathione level during vegetative growth

References:

Genotypes:

FYPO:0003270 - growth auxotrophic for glutathione

References:

Genotypes:

FYPO:0003862 - increased protein oxidation in stationary phase

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0000740 - normal cellular pigment accumulation

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000108 - sensitive to menadione

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000013 - RNA level unchanged

References:

PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000354 - abnormal endoplasmic reticulum morphology

References:

Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0005883 - abolished anaerobic cell population growth

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Genotypes:

FYPO:0001934 - abolished cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0002854 - abolished glutamate-cysteine ligase activity

References:

Genotypes:

FYPO:0010034 - abolished nuclear pore clustering during cellular response to hydroxyurea

References:

Genotypes:

FYPO:0000249 - decreased cell population growth on ammonia nitrogen source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

References:

Genotypes:

FYPO:0001570 - decreased cellular glutathione level during vegetative growth

References:

Genotypes:

FYPO:0002855 - decreased glutamate-cysteine ligase activity

References:

Genotypes:

FYPO:0001422 - decreased protein processing during vegetative growth

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003270 - growth auxotrophic for glutathione

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0003004 - increased cellular reactive oxygen species level during vegetative growth

References:

Genotypes:

FYPO:0005653 - increased level of iron ion starvation-induced proteins during glutathione starvation

References:

Genotypes:

FYPO:0002014 - increased RNA level during cellular response to iron ion starvation

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001753 - normal anaerobic cell population growth

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Genotypes:

FYPO:0001437 - normal growth on antimycin A

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Genotypes:

FYPO:0003506 - normal growth on copper

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0002691 - normal growth on menadione

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Genotypes:

FYPO:0005884 - normal growth on myxothiazol

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Genotypes:

FYPO:0001691 - normal growth on paraquat

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Genotypes:

FYPO:0005639 - normal RNA level during cellular response to iron ion starvation

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Genotypes:

FYPO:0005881 - ophthalmic acid absent from cell

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Genotypes:

FYPO:0002722 - phytochelatin absent from cell

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Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009034 - resistance to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0004513 - resistance to latrunculin A

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Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000077 - resistance to rapamycin

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Genotypes:

FYPO:0009040 - resistance to tea tree oil

References:

Genotypes:

FYPO:0008287 - sensitive to 1-chloro-2,4-dinitrobenzene

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000108 - sensitive to menadione

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000271 - sensitive to salt stress

References:

Genotypes:

FYPO:0000750 - sensitive to silver ions

References:

Genotypes:

FYPO:0006929 - sensitive to silver nanoparticles

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF03074GCSGCSPfam
G3DSA:3.30.590.50:FF:000002CATH-FunFam
G3DSA:1.10.8.960CATH-Gene3D
G3DSA:3.30.590.50CATH-Gene3D
SSF50249NA-bd_OB-foldSUPERFAMILY
SSF55931Gln_synth/guanido_kin_cat_domSUPERFAMILY
PTHR11164GCSPANTHER

Orthologs

References / Literature

PMID:7608113 - Molecular cloning and nucleotide sequencing of the gamma-glutamylcysteine synthetase gene of the fission yeast Schizosaccharomyces pombe.
Mutoh N et al. J Biochem 1995 Feb;117(2):283-8
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:17986764 - Accelerated chronological aging of a mutant fission yeast deficient in both glutathione and superoxide dismutase having cu and zn as cofactors and its enhancement by sir2 deficiency.
Mutoh N et al. Biosci Biotechnol Biochem 2007 Nov;71(11):2841-4
PMID:41259369 - Hydroxyurea induces an oxidative stress response that triggers ER expansion and cytoplasmic protein aggregation.
Sánchez-Molina A et al. PLoS Biol 2025 Nov 19;23(11):e3003493
PMID:25552606 - Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. G3 (Bethesda) 2014 Dec 31;5(3):361-70
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:9017391 - apd1+, a gene required for red pigment formation in ade6 mutants of Schizosaccharomyces pombe, encodes an enzyme required for glutathione biosynthesis: a role for glutathione and a glutathione-conjugate pump.
Chaudhuri B et al. Genetics 1997 Jan;145(1):75-83
PMID:25806539 - A cascade of iron-containing proteins governs the genetic iron starvation response to promote iron uptake and inhibit iron storage in fission yeast.
Encinar del Dedo J et al. PLoS Genet 2015 Mar;11(3):e1005106
PMID:29294138 - Editor's Highlight: A Genome-wide Screening of Target Genes Against Silver Nanoparticles in Fission Yeast.
Lee AR et al. Toxicol Sci 2018 Jan 01;161(1):171-185
PMID:2894829 - Isolation of mutants of Schizosaccharomyces pombe unable to synthesize cadystin, small cadmium-binding peptides.
Mutoh N et al. Biochem Biophys Res Commun 1988 Feb 29;151(1):32-9
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:18665268 - Mitochondrial dysfunction increases oxidative stress and decreases chronological life span in fission yeast.
Zuin A et al. PLoS One 2008 Jul 30;3(7):e2842
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16428309 - Effects of four oxidants, menadione, 1-chloro-2,4-dinitrobenzene, hydrogen peroxide and cumene hydroperoxide, on fission yeast Schizosaccharmoyces pombe.
Mutoh N et al. J Biochem 2005 Dec;138(6):797-804
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:8619315 - Gcs1, a gene encoding gamma-glutamylcysteine synthetase in the fission yeast Schizosaccharomyces pombe.
Coblenz A et al. Yeast 1995 Sep 30;11(12):1171-7
PMID:27005325 - Diverse fission yeast genes required for responding to oxidative and metal stress: Comparative analysis of glutathione-related and other defense gene deletions.
Pluskal T et al. Genes Cells 2016 Jun;21(6):530-42
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:28640807 - Lack of a peroxiredoxin suppresses the lethality of cells devoid of electron donors by channelling electrons to oxidized ribonucleotide reductase.
Boronat S et al. PLoS Genet 2017 Jun;13(6):e1006858
PMID:18223116 - Key function for the CCAAT-binding factor Php4 to regulate gene expression in response to iron deficiency in fission yeast.
Mercier A et al. Eukaryot Cell 2008 Mar;7(3):493-508
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:15040451 - Stress-dependent regulation of the gene encoding gamma-glutamylcysteine synthetase from the fission yeast.
Kim SJ et al. Mol Biol Rep 2004 Mar;31(1):23-30
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24118096 - Methionine sulphoxide reductases revisited: free methionine as a primary target of H₂O₂stress in auxotrophic fission yeast.
García-Santamarina S et al. Mol Microbiol 2013 Dec;90(5):1113-24
PMID:27053105 - Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast.
Burr R et al. J Biol Chem 2016 Jun 03;291(23):12171-83
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160