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protein coding gene - ura1 (SPAC22G7.06c) - trifunctional GLNase (glutamine amidotransferase)/CPSase (carbamoylphosphate synthetase)/ATCase (aspartate transcarbamoylase) Ura1

Gene summary

Standard name
ura1
Systematic ID
SPAC22G7.06c
Product
trifunctional GLNase (glutamine amidotransferase)/CPSase (carbamoylphosphate synthetase)/ATCase (aspartate transcarbamoylase) Ura1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09794
ORFeome ID
34/34C07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 739167..746114 reverse strand

Annotation

Complementation

PBO:0091277 - functionally complements E. coli pyrB

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Disease association

MONDO:0014647 - developmental and epileptic encephalopathy, 50

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GO biological process

GO:0006207 - 'de novo' pyrimidine nucleobase biosynthetic process

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GO:0044205 - 'de novo' UMP biosynthetic process

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GO:0006177 - GMP biosynthetic process

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GO:0006541 - L-glutamine metabolic process

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GO cellular component

GO:0005829 - cytosol

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GO molecular function

GO:0016597 - amino acid binding

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GO:0004070 - aspartate carbamoyltransferase activity

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GO:0005524 - ATP binding

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GO:0004087 - carbamoyl-phosphate synthase (ammonia) activity

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GO:0004088 - carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity

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GO:0004359 - glutaminase activity

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GO:0003922 - GMP synthase (glutamine-hydrolyzing) activity

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GO:0046872 - metal ion binding

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GO:0004151 - dihydroorotase activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000027 - ribosomal density decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

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Genotypes:

FYPO:0009078 - decreased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

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Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

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Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

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Genotypes:

FYPO:0001012 - growth auxotrophic for uracil

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Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005968 - resistance to sodium chloride

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0002865 - sensitive to polypeptone

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0006016 - sensitive to urea

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00117GATaseGATASEPfam
PF00185OTCaceAsp_carbamoyltransf_Asp/Orn-bdPfam
PF00988CPSase_sm_chainCarbamoylP_synth_ssu_NPfam
PF02142MGSMGS-like_domPfam
PF02729OTCace_NAsp/Orn_carbamoyltranf_P-bdPfam
PF02786CPSase_L_D2CPAse_ATP-bdPfam
PF02787CPSase_L_D3CPSase_lsu_oligoPfam
PF25596CPSase_L_D1CPSase_preATP-graspPfam
cd01316CAD_DHOaseCDD
cd01423MGS_CPS_I_IIICDD
cd01744GATase1_CPSaseCPSase_GATase1CDD
PS00097CARBAMOYLTRANSFERASEAsp/Orn_carbamoylTrfasePROSITE patterns
PS00866CPSASE_1CPAse_ATP-bdPROSITE patterns
PS00867CPSASE_2CPAse_ATP-bdPROSITE patterns
PS50975ATP_GRASPATP-graspPROSITE profiles
PS51273GATASE_TYPE_1PROSITE profiles
PS51855MGSMGS-like_domPROSITE profiles
SM00851MGSMGS-like_domSMART
SM01096CPSase_L_D3CPSase_lsu_oligoSMART
SM01097CPSase_sm_chainCarbamoylP_synth_ssu_NSMART
PR00098CPSASECPSase_domPRINTS
PR00099CPSGATASEPRINTS
PR00100AOTCASEAsp/Orn_carbamoylTrfasePRINTS
PR00101ATCASEPRINTS
G3DSA:1.10.1030.10:FF:000001CATH-FunFam
G3DSA:3.20.20.140:FF:000036CATH-FunFam
G3DSA:3.30.1490.20:FF:000001CATH-FunFam
G3DSA:3.30.470.20:FF:000001CATH-FunFam
G3DSA:3.30.470.20:FF:000004CATH-FunFam
G3DSA:3.40.50.1370:FF:000002CATH-FunFam
G3DSA:3.40.50.1370:FF:000005CATH-FunFam
G3DSA:3.40.50.1380:FF:000005CATH-FunFam
G3DSA:3.40.50.20:FF:000002CATH-FunFam
G3DSA:3.40.50.20:FF:000011CATH-FunFam
G3DSA:3.40.50.880:FF:000025CATH-FunFam
G3DSA:3.50.30.20:FF:000002CATH-FunFam
G3DSA:1.10.1030.10CarbamoylP_synth_lsu_oligo_sfCATH-Gene3D
G3DSA:3.20.20.140CATH-Gene3D
G3DSA:3.30.1490.20ATP_grasp_subdomain_1CATH-Gene3D
G3DSA:3.30.470.20CATH-Gene3D
G3DSA:3.40.50.1370Asp/Orn_carbamoylTrfase_sfCATH-Gene3D
G3DSA:3.40.50.1380MGS-like_dom_sfCATH-Gene3D
G3DSA:3.40.50.20CATH-Gene3D
G3DSA:3.40.50.880Class_I_gatase-likeCATH-Gene3D
G3DSA:3.50.30.20CarbP_synth_ssu_N_sfCATH-Gene3D
SSF48108CarbamoylP_synth_lsu_oligo_sfSUPERFAMILY
SSF51338Metal-dep_hydrolase_compositeSUPERFAMILY
SSF51556Metal_HydrolaseSUPERFAMILY
SSF52021CarbP_synth_ssu_N_sfSUPERFAMILY
SSF52317Class_I_gatase-likeSUPERFAMILY
SSF52335MGS-like_dom_sfSUPERFAMILY
SSF52440PreATP-grasp_dom_sfSUPERFAMILY
SSF53671Asp/Orn_carbamoylTrfase_sfSUPERFAMILY
SSF56059SUPERFAMILY
PTHR11405PANTHER
NF002032PRK00856.1NCBIFAM
NF003671carBNCBIFAM
NF009455PRK12815.1NCBIFAM
NF009475PRK12838.1NCBIFAM
TIGR00670asp_carb_trAsp_carbamoyltransfNCBIFAM
TIGR01368CPSaseIIsmallCarbamoylP_synth_ssuNCBIFAM
TIGR01369CPSaseII_lrgCPSase_lsuNCBIFAM
MF_00001Asp_carb_trAsp_carbamoyltransfHAMAP
MF_01209CPSase_S_chainCarbamoylP_synth_ssuHAMAP
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder

Orthologs

References / Literature

GO_REF:0000003 - Gene Ontology annotation based on Enzyme Commission mapping
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000002 - Comments
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:6961452 - Cloned ural locus of Schizosaccharomyces pombe propagates autonomously in this yeast assuming a polymeric form.
Sakaguchi J et al. Proc Natl Acad Sci U S A 1982 Dec;79(24):7819-23
PMID:8580465 - The beta 7 integrins in mucosal homing and retention.
Shaw SK et al. Semin Immunol 1995 Oct;7(5):335-42
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
GO_REF:0000041 - Gene Ontology annotation based on UniPathway vocabulary mapping.
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:8590465 - As in Saccharomyces cerevisiae, aspartate transcarbamoylase is assembled on a multifunctional protein including a dihydroorotase-like cryptic domain in Schizosaccharomyces pombe.
Lollier M et al. Curr Genet 1995 Jul;28(2):138-49
PMID:11864600 - Meiotic recombination remote from prominent DNA break sites in S. pombe.
Young JA et al. Mol Cell 2002 Feb;9(2):253-63
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:20976105 - Silencing mediated by the Schizosaccharomyces pombe HIRA complex is dependent upon the Hpc2-like protein, Hip4.
Anderson HE et al. PLoS One 2010 Oct 18;5(10):e13488
GO_REF:0000116 - Automatic Gene Ontology annotation based on Rhea mapping.
PMID:36799444 - Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast.
Hirano Y et al. J Biochem 2023 Jun 30;174(1):33-46
PMID:37851576 - The LARP1 homolog Slr1p controls the stability and expression of proto-5'TOP mRNAs in fission yeast.
Mansouri-Noori F et al. Cell Rep 2023 Oct 31;42(10):113226
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:9211790 - Caffeine-resistance in S. pombe: mutations in three novel caf genes increase caffeine tolerance and affect radiation sensitivity, fertility, and cell cycle.
Benko Z et al. Curr Genet 1997 Jun;31(6):481-7
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:9301025 - Mapping of ure1, ure2 and ure3 markers in fission yeast.
Lubbers MW et al. Yeast 1997 Sep 30;13(12):1195-7
GO_REF:0000108 - Automatic assignment of GO terms using logical inference, based on on inter-ontology links.
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:20625380 - A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length.
Liu NN et al. Cell Res 2010 Aug;20(8):963-5
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:17248775 - Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.
Kohli J et al. Genetics 1977 Nov;87(3):471-89
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.
Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:23555823 - Polypeptone induces dramatic cell lysis in ura4 deletion mutants of fission yeast.
Matsuo Y et al. PLoS One 2013;8(3):e59887
PMID:26152728 - A Two-step Protein Quality Control Pathway for a Misfolded DJ-1 Variant in Fission Yeast.
Mathiassen SG et al. J Biol Chem 2015 Aug 21;290(34):21141-21153
PMID:28345447 - Urea enhances cell lysis of Schizosaccharomyces pombe ura4 mutants.
Nishino K et al. Biosci Biotechnol Biochem 2017 Jul;81(7):1444-1451