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protein coding gene - scd2 (SPAC22H10.07) - Cdc42 GTPase complex scaffold subunit Scd2

Gene summary

Standard name
scd2
Systematic ID
SPAC22H10.07
Product
Cdc42 GTPase complex scaffold subunit Scd2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ral3
UniProt ID
P40996
ORFeome ID
34/34H02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2382504..2385153 forward strand

Annotation

PBO:0000384 - Ras1-Scd

Disease association

MONDO:0009579 - Frank-Ter Haar syndrome

References:

GO biological process

GO:0030010 - establishment of cell polarity

References:

GO:1902917 - positive regulation of mating projection assembly

References:

GO:0031137 - regulation of conjugation with cellular fusion

References:

GO:0032878 - regulation of establishment or maintenance of cell polarity

References:

GO cellular component

GO:0071521 - Cdc42 GTPase complex

References:

GO:0032153 - cell division site

References:

GO:0051286 - cell tip

References:

GO:0090726 - cortical dynamic polarity patch

References:

GO:0005829 - cytosol

References:

GO:1990819 - mating projection actin fusion focus

References:

GO:0043332 - mating projection tip

References:

GO:0005634 - nucleus

References:

GO:0031520 - plasma membrane of cell tip

References:

GO molecular function

GO:0035091 - phosphatidylinositol binding

References:

GO:0005515 - protein binding

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GO:0140378 - protein complex scaffold activity

References:

GO:0030674 - protein-macromolecule adaptor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0000252 - increased spontaneous diploidization

References:

Genotypes:

FYPO:0001587 - normal protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0003316 - normal protein localization to growing cell tip

References:

Genotypes:

FYPO:0007377 - protein mislocalized to medial cortex during mitotic interphase

References:

Genotypes:

FYPO:0005466 - protein mislocalized to non-growing cell tip

References:

Genotypes:

FYPO:0000013 - T-shaped vegetative cell with normal cell length

References:

Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

References:

Genotypes:

FYPO:0006616 - viable vegetative cell with increased cell diameter

References:

Genotypes:

FYPO:0002104 - viable vegetative cell with normal cell shape

References:

Genotypes:

Protein features

PBO:0111783 - PX domain protein

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0005501 - abolished protein localization to cell cortex, with protein mislocalized to cytoplasm, during vegetative growth

References:

Genotypes:

FYPO:0001880 - abolished protein localization to cell division site

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

References:

Genotypes:

FYPO:0000303 - decreased conjugation frequency

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0001586 - decreased protein localization to cell tip during vegetative growth

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Genotypes:

FYPO:0002871 - decreased protein localization to growing cell tip

References:

Genotypes:

FYPO:0005803 - decreased rate of protein movement within plasma membrane at cell side

References:

Genotypes:

FYPO:0000584 - decreased sporulation frequency

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0009074 - increased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0000782 - mislocalized protein during vegetative growth

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Genotypes:

FYPO:0001294 - normal actin cortical patch localization

References:

Genotypes:

FYPO:0003135 - normal agglutination

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Genotypes:

FYPO:0001293 - normal cell wall biogenesis

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Genotypes:

FYPO:0000644 - normal protein localization during vegetative growth

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Genotypes:

FYPO:0000590 - normal sporulation

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0005466 - protein mislocalized to non-growing cell tip

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0005969 - resistance to magnesium chloride

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009039 - resistance to potassium chloride

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Genotypes:

FYPO:0000327 - resistance to trichostatin A

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0000093 - sensitive to arsenic

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Genotypes:

FYPO:0007921 - sensitive to benzamidine

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009071 - sensitive to itraconazole

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0000280 - sterile

References:

Genotypes:

FYPO:0000024 - stubby vegetative cell

References:

Genotypes:

FYPO:0006617 - viable elongated vegetative cell with increased cell diameter

References:

Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

References:

Genotypes:

FYPO:0002106 - viable stubby vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002197 - viable vegetative cell with abnormal cell shape

References:

Genotypes:

FYPO:0006616 - viable vegetative cell with increased cell diameter

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00787PXPX_domPFAM
PF00018SH3_1SH3_domainPFAM
cd05992PB1CDD
cd06890PX_Bem1pBem1/Scd2_PXCDD
cd11879SH3_Bem1p_2Bem1/Scd2_SH3_2CDD
cd11878SH3_Bem1p_1Bem1/Scd2_SH3_1CDD
PS00018EF_HAND_1EF_Hand_1_Ca_BSPROSITE_PATTERNS
PS50195PXPX_domPROSITE_PROFILES
PS51745PB1PB1-likePROSITE_PROFILES
PS50002SH3SH3_domainPROSITE_PROFILES
SM00666PB1_newPB1_domSMART
SM00326SH3_2SH3_domainSMART
SM00312PX_2PX_domSMART
G3DSA:2.30.30.40:FF:000093FUNFAM
SSF50044SH3-domainSH3-like_dom_sfSUPERFAMILY
SSF64268PX domainPX_dom_sfSUPERFAMILY
SSF54277CAD & PB1 domainsSUPERFAMILY
G3DSA:2.30.30.40SH3 DomainsGENE3D
G3DSA:3.30.1520.10PX_dom_sfGENE3D
G3DSA:3.10.20.90GENE3D
PTHR15706SH3 MULTIPLE DOMAINNADPH_Oxidase/PX-DomainPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity

Orthologs

References / Literature

PMID:9660817 - Cloning and characterization of shk2, a gene encoding a novel p21-activated protein kinase from fission yeast.
Yang P et al. J Biol Chem 1998 Jul 17;273(29):18481-9
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:41335110 - High-resolution mapping of the actin fusion focus reveals myosin V-dependent formin transport for aster formation.
Thomas V et al. J Cell Biol 2026 Feb 02;225(2)
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23200991 - Cdc42 explores the cell periphery for mate selection in fission yeast.
Bendezú FO et al. Curr Biol 2013 Jan 07;23(1):42-7
PMID:30279276 - Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction.
Gallo Castro D et al. J Cell Biol 2018 Dec 03;217(12):4215-4229
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:31719163 - A novel interplay between GEFs orchestrates Cdc42 activity during cell polarity and cytokinesis in fission yeast.
Hercyk BS et al. J Cell Sci 2019 Dec 03;132(23)
PMID:25837586 - Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking.
Bendezú FO et al. PLoS Biol 2015 Apr;13(4):e1002097
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:9892665 - Moe1, a conserved protein in Schizosaccharomyces pombe, interacts with a Ras effector, Scd1, to affect proper spindle formation.
Chen CR et al. Proc Natl Acad Sci U S A 1999 Jan 19;96(2):517-22
PMID:23615447 - Compartmentalized nodes control mitotic entry signaling in fission yeast.
Deng L et al. Mol Biol Cell 2013 Jun;24(12):1872-81
PMID:12409291 - The Cdc42 binding and scaffolding activities of the fission yeast adaptor protein Scd2.
Endo M et al. J Biol Chem 2003 Jan 10;278(2):843-52
PMID:9133664 - The Schizosaccharomyces pombe mra1 gene, which is required for cell growth and mating, can suppress the mating inefficiency caused by a deficit in the Ras1 activity.
Hakuno F et al. Genes Cells 1996 Mar;1(3):303-15
PMID:8943016 - The highly conserved skb1 gene encodes a protein that interacts with Shk1, a fission yeast Ste20/PAK homolog.
Gilbreth M et al. Proc Natl Acad Sci U S A 1996 Nov 26;93(24):13802-7
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:21849474 - Spatial control of Cdc42 activation determines cell width in fission yeast.
Kelly FD et al. Mol Biol Cell 2011 Oct;22(20):3801-11
PMID:3071741 - Isolation and characterization of Schizosaccharomyces pombe mutants phenotypically similar to ras1-.
Fukui Y et al. Mol Gen Genet 1988 Dec;215(1):26-31
PMID:11483497 - Novel modular domain PB1 recognizes PC motif to mediate functional protein-protein interactions.
Ito T et al. EMBO J 2001 Aug 01;20(15):3938-46
PMID:15631622 - Interactions between Cdc42 and the scaffold protein Scd2: requirement of SH3 domains for GTPase binding.
Wheatley E et al. Biochem J 2005 May 15;388(Pt 1):177-84
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:10567532 - Direct binding and In vivo regulation of the fission yeast p21-activated kinase shk1 by the SH3 domain protein scd2.
Chang E et al. Mol Cell Biol 1999 Dec;19(12):8066-74
PMID:27558664 - Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.
Guo L et al. G3 (Bethesda) 2016 Oct 13;6(10):3317-3333
PMID:16615890 - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.
Chikashige Y et al. Cell 2006 Apr 07;125(1):59-69
PMID:27746023 - Remodeling of the Fission Yeast Cdc42 Cell-Polarity Module via the Sty1 p38 Stress-Activated Protein Kinase Pathway.
Mutavchiev DR et al. Curr Biol 2016 Nov 07;26(21):2921-2928
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:9182664 - Type II myosin heavy chain encoded by the myo2 gene composes the contractile ring during cytokinesis in Schizosaccharomyces pombe.
Kitayama C et al. J Cell Biol 1997 Jun 16;137(6):1309-19
PMID:9679144 - Regulation of cell polarity by microtubules in fission yeast.
Sawin KE et al. J Cell Biol 1998 Jul 27;142(2):457-71
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:39012625 - Arp2/3-dependent endocytosis ensures Cdc42 oscillations by removing Pak1-mediated negative feedback.
Harrell MA et al. J Cell Biol 2024 Sep 02;223(10)
PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.
Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:27022830 - Microscopy of Fission Yeast Sexual Lifecycle.
Vjestica A et al. J Vis Exp 2016 Mar 09;(109)
PMID:7923372 - Cooperative interaction of S. pombe proteins required for mating and morphogenesis.
Chang EC et al. Cell 1994 Oct 07;79(1):131-41
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:10574765 - Mis-specification of cortical identity in a fission yeast PAK mutant.
Sawin KE et al. Curr Biol 1999 Nov 18;9(22):1335-8
PMID:19710424 - Pob1 participates in the Cdc42 regulation of fission yeast actin cytoskeleton.
Rincón SA et al. Mol Biol Cell 2009 Oct;20(20):4390-9
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721