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protein coding gene - alp21 (SPAC22H10.10) - tubulin specific chaperone cofactor E

Gene summary

Standard name
alp21
Systematic ID
SPAC22H10.10
Product
tubulin specific chaperone cofactor E
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
sto1
UniProt ID
Q10303
ORFeome ID
35/35D11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2389936..2391943 forward strand

Annotation

Complementation

PBO:0123294 - is not functionally complemented by human TBCE

References:

Disease association

MONDO:0009486 - autosomal recessive Kenny-Caffey syndrome

References:

MONDO:0014968 - encephalopathy, progressive, with amyotrophy and optic atrophy

References:

MONDO:0009426 - hypoparathyroidism-retardation-dysmorphism syndrome

References:

GO biological process

GO:0000226 - microtubule cytoskeleton organization

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GO:0007023 - post-chaperonin tubulin folding pathway

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0043014 - alpha-tubulin binding

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Multi-locus phenotype

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

Protein features

PBO:0111865 - leucine-rich repeat protein

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000229 - cut

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Genotypes:

FYPO:0000158 - DNA content increased during vegetative growth

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Genotypes:

FYPO:0001272 - incomplete, unequal mitotic sister chromatid segregation, with smeared DNA

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Genotypes:

FYPO:0002262 - inviable after spore germination, multiple cell divisions, elongated cell

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Genotypes:

FYPO:0001042 - inviable after spore germination, single or double cell division

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Genotypes:

FYPO:0004522 - inviable after spore germination, single or double cell division, branched, curved, elongated cell

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Genotypes:

FYPO:0000313 - inviable after spore germination, without cell division, with abnormal germ tube morphology

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Genotypes:

FYPO:0002303 - inviable mononucleate monoseptate vegetative cell with anucleate compartment

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002818 - microtubule bundles present in decreased numbers

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Genotypes:

FYPO:0002018 - mitotic spindle absent from cell

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0000899 - normal microtubule cytoskeleton organization during vegetative growth

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PS51450LRRLeu-rich_rptPROSITE_PROFILES
SSF52058L domain-likeSUPERFAMILY
G3DSA:3.80.10.10Ribonuclease InhibitorLRR_dom_sfGENE3D
PTHR45617LEUCINE RICH REPEAT FAMILY PROTEINPANTHER

Orthologs

References / Literature

PMID:10978278 - The cofactor-dependent pathways for alpha- and beta-tubulins in microtubule biogenesis are functionally different in fission yeast.
Radcliffe PA et al. Genetics 2000 Sep;156(1):93-103
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:17004072 - Chromosome segregation in fission yeast with mutations in the tubulin folding cofactor D.
Fedyanina OS et al. Curr Genet 2006 Nov;50(5):281-94
PMID:23576550 - The dual role of fission yeast Tbc1/cofactor C orchestrates microtubule homeostasis in tubulin folding and acts as a GAP for GTPase Alp41/Arl2.
Mori R et al. Mol Biol Cell 2013 Jun;24(11):1713-24, S1-8
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.
Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:10341216 - Sto1p, a fission yeast protein similar to tubulin folding cofactor E, plays an essential role in mitotic microtubule assembly.
Grishchuk EL et al. J Cell Sci 1999 Jun;112 ( Pt 12):1979-88
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:10473641 - Functional dissection and hierarchy of tubulin-folding cofactor homologues in fission yeast.
Radcliffe PA et al. Mol Biol Cell 1999 Sep;10(9):2987-3001
PMID:10683446 - A conserved small GTP-binding protein Alp41 is essential for the cofactor-dependent biogenesis of microtubules in fission yeast.
Radcliffe PA et al. FEBS Lett 2000 Feb 18;468(1):84-8