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protein coding gene - mnl1 (SPAC23A1.04c) - alpha mannosidase Mnl1

Gene summary

Standard name
mnl1
Systematic ID
SPAC23A1.04c
Product
alpha mannosidase Mnl1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
htm1
UniProt ID
O94726
ORFeome ID
28/28F12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4084455..4087646 reverse strand

Annotation

GO biological process

GO:1904380 - endoplasmic reticulum mannose trimming

References:

GO:0097466 - ubiquitin-dependent glycoprotein ERAD pathway

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005783 - endoplasmic reticulum

References:

GO:0016020 - membrane

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GO molecular function

GO:0005509 - calcium ion binding

References:

GO:0030246 - carbohydrate binding

References:

GO:0004571 - mannosyl-oligosaccharide 1,2-alpha-mannosidase activity

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

Protein sequence feature

SO:0000418 - signal_peptide

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0009097 - decreased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0001146 - decreased misfolded glycoprotein degradation during vegetative growth

References:

Genotypes:

FYPO:0001145 - increased Man9GlcNAc level

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001142 - normal Man8GlcNAc level

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01532Glyco_hydro_47Glyco_hydro_47PFAM
PR00747GLYHDRLASE47Glyco_hydro_47PRINTS
SSF48225Seven-hairpin glycosidasesSeven-hairpin_glycosidasesSUPERFAMILY
G3DSA:1.50.10.106hp_glycosidase-like_sfGENE3D
PTHR45679ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2EDEM1/2/3PANTHER

Orthologs

References / Literature

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
GO_REF:0000002 - Comments
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:16079177 - Characterization of Schizosaccharomyces pombe ER alpha-mannosidase: a reevaluation of the role of the enzyme on ER-associated degradation.
Movsichoff F et al. Mol Biol Cell 2005 Oct;16(10):4714-24
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160