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protein coding gene - cmk2 (SPAC23A1.06c) - MAPK-activated protein kinase Cmk2

Gene summary

Standard name
cmk2
Systematic ID
SPAC23A1.06c
Product
MAPK-activated protein kinase Cmk2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
mkp2
UniProt ID
O42844
ORFeome ID
26/26G08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4089794..4092099 reverse strand

Annotation

GO biological process

GO:0034599 - cellular response to oxidative stress

References:

GO:0010972 - negative regulation of G2/M transition of mitotic cell cycle

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GO cellular component

GO:0072324 - ascus epiplasm

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GO:0005938 - cell cortex

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GO:0051285 - cell cortex of cell tip

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GO:0005737 - cytoplasm

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GO:0000935 - division septum

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GO:0005628 - prospore membrane

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GO:0030479 - actin cortical patch

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GO molecular function

GO:0004683 - calcium/calmodulin-dependent protein kinase activity

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GO:0005515 - protein binding

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GO:0004672 - protein kinase activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0004693 - normal viability following cellular response to hydroxyurea

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Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

Qualitative gene expression

PomGeneEx:0000024 - protein level fluctuates

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PomGeneEx:0000021 - protein present

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

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Genotypes:

FYPO:0003020 - abolished protein autophosphorylation during vegetative growth

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Genotypes:

FYPO:0001384 - abolished protein kinase activity

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Genotypes:

FYPO:0006978 - decreased cellular coenzyme Q10 level

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Genotypes:

FYPO:0001128 - decreased septation index

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0009007 - decreased vegetative cell population viability

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Genotypes:

FYPO:0003532 - increased monopolar index

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Genotypes:

FYPO:0004344 - increased viability upon nitrogen starvation

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Genotypes:

FYPO:0001497 - inviable elongated cell with mitotic cell cycle arrest in interphase

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0004295 - multiseptate cell

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Genotypes:

FYPO:0006237 - normal cytosolic half-mer polysome level

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Genotypes:

FYPO:0006236 - normal cytosolic monomeric ribosome level

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Genotypes:

FYPO:0001037 - normal growth during cellular response to salt stress

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Genotypes:

FYPO:0001020 - normal growth on calcium

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Genotypes:

FYPO:0001147 - normal mating efficiency

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0001450 - resistance to cold

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0000726 - sensitive to oxidative stress

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0006156 - sensitive to sodium arsenite

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Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
PF00069PkinaseProt_kinase_domPFAM
PS00108PROTEIN_KINASE_STSer/Thr_kinase_ASPROSITE_PATTERNS
PS00107PROTEIN_KINASE_ATPProtein_kinase_ATP_BSPROSITE_PATTERNS
PS50011PROTEIN_KINASE_DOMProt_kinase_domPROSITE_PROFILES
SM00220serkin_6Prot_kinase_domSMART
G3DSA:3.30.200.20:FF:001875FUNFAM
SSF56112Protein kinase-like (PK-like)Kinase-like_dom_sfSUPERFAMILY
G3DSA:1.10.510.10Transferase(Phosphotransferase) domain 1GENE3D
G3DSA:3.30.200.20Phosphorylase Kinase; domain 1GENE3D
PTHR24347SERINE/THREONINE-PROTEIN KINASEPANTHER

Orthologs

References / Literature

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:18065650 - Fission yeast mitogen-activated protein kinase Sty1 interacts with translation factors.
Asp E et al. Eukaryot Cell 2008 Feb;7(2):328-38
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:31030285 - CoQ 10 production in Schizosaccharomyces pombe is increased by reduction of glucose levels or deletion of pka1.
Nishida I et al. Appl Microbiol Biotechnol 2019 Jun;103(12):4899-4915
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:15821139 - Systematic deletion analysis of fission yeast protein kinases.
Bimbó A et al. Eukaryot Cell 2005 Apr;4(4):799-813
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:12589433 - Mkp1 and Mkp2, two MAPKAP-kinase homologues in Schizosaccharomyces pombe, interact with the MAP kinase Sty1.
Asp E et al. Mol Genet Genomics 2003 Feb;268(5):585-97
PMID:15870269 - Response of fission yeast to toxic cations involves cooperative action of the stress-activated protein kinase Spc1/Sty1 and the Hal4 protein kinase.
Wang LY et al. Mol Cell Biol 2005 May;25(10):3945-55
PMID:30236788 - Cmk2 kinase is essential for survival in arsenite by modulating translation together with RACK1 orthologue Cpc2 in Schizosaccharomyces pombe.
Sanchez-Marinas M et al. Free Radic Biol Med 2018 Dec;129:116-126
PMID:34984977 - Functional profiling of long intergenic non-coding RNAs in fission yeast.
Rodriguez-Lopez M et al. Elife 2022 Jan 05;11
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:11886858 - The serine/threonine kinase Cmk2 is required for oxidative stress response in fission yeast.
Sánchez-Piris M et al. J Biol Chem 2002 May 17;277(20):17722-7
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
GO_REF:0000003 - Gene Ontology annotation based on Enzyme Commission mapping
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:20501954 - Search for kinases related to transition of growth polarity in fission yeast.
Koyano T et al. Biosci Biotechnol Biochem 2010;74(5):1129-33
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:26575035 - Ssp1 CaMKK: A Sensor of Actin Polarization That Controls Mitotic Commitment through Srk1 in Schizosaccharomyces pombe.
Gómez-Hierro A et al. PLoS One 2015;10(11):e0143037
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:20444100 - Hyperosmosis enhances radiation and hydroxyurea resistance of Schizosaccharomyces pombe checkpoint mutants through the spindle checkpoint and delayed cytokinesis.
Alao JP et al. Mol Microbiol 2010 Jul 01;77(1):143-57
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:12135745 - Cmk2, a novel serine/threonine kinase in fission yeast.
Alemany V et al. FEBS Lett 2002 Jul 31;524(1-3):79-86
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36