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protein coding gene - tef102 (SPAC23A1.10) - translation elongation factor EF-1 alpha Ef1a-b

Gene summary

Standard name
tef102
Systematic ID
SPAC23A1.10
Product
translation elongation factor EF-1 alpha Ef1a-b
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ef1a-b, efa1, EF-1aC, tef1-b, tef1-d, ef1a-d, SPBC24E9.15c
UniProt ID
P0CT54
ORFeome ID
21/21D07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 4095132..4096584 forward strand

Annotation

Disease association

MONDO:0014625 - developmental and epileptic encephalopathy, 33

References:

MONDO:0014617 - intellectual disability, autosomal dominant 38

References:

GO biological process

GO:0002182 - cytoplasmic translational elongation

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0005853 - eukaryotic translation elongation factor 1 complex

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GO molecular function

GO:0005516 - calmodulin binding

References:

GO:0005525 - GTP binding

References:

GO:0003924 - GTPase activity

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GO:0003746 - translation elongation factor activity

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Modification

MOD:02025 - 5-glutaminyl glutamic acid

References:

MOD:01982 - N,N,N-trimethylglycine

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MOD:00006 - N-glycosylated residue

References:

MOD:00083 - N6,N6,N6-trimethyl-L-lysine

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MOD:00084 - N6,N6-dimethyl-L-lysine

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MOD:00085 - N6-methyl-L-lysine

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MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Qualitative gene expression

PomGeneEx:0000027 - ribosomal density decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000013 - RNA level unchanged

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PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001418 - abnormal microtubule cytoskeleton morphology during vegetative growth

References:

Genotypes:

FYPO:0000026 - abnormal vegetative cell polarity

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0002021 - dispersed actin cortical patch localization during vegetative growth

References:

Genotypes:

FYPO:0003618 - normal growth on aculeacin A

References:

Genotypes:

FYPO:0001236 - normal growth on cycloheximide

References:

Genotypes:

FYPO:0003617 - sensitive to cytochalasin D

References:

Genotypes:

FYPO:0003616 - sensitive to fluphenazine

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0002459 - viable branched, elongated vegetative cell

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Genotypes:

FYPO:0002112 - viable curved vegetative cell

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Genotypes:

FYPO:0002377 - viable swollen vegetative cell

References:

Genotypes:

FYPO:0001491 - viable vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00009GTP_EFTUT_Tr_GTP-bd_domPFAM
PF22594GTP-eEF1A_CGTP-eEF1A_CPFAM
PF03144GTP_EFTU_D2EFTu-like_2PFAM
cd03705EF1_alpha_IIICDD
cd03693EF1_alpha_IICDD
cd01883EF1_alphaCDD
PS00301G_TR_1G_TR_CSPROSITE_PATTERNS
PS51722G_TR_2T_Tr_GTP-bd_domPROSITE_PROFILES
PR00315ELONGATNFCTT_Tr_GTP-bd_domPRINTS
G3DSA:2.40.30.10:FF:000003FUNFAM
G3DSA:3.40.50.300:FF:000211FUNFAM
G3DSA:2.40.30.10:FF:000005FUNFAM
SSF50447Translation proteinsTransl_B-barrel_sfSUPERFAMILY
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
SSF50465EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domainTransl_elong_EF1A/Init_IF2_CSUPERFAMILY
G3DSA:2.40.30.10Translation factorsGENE3D
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
PTHR23115TRANSLATION FACTORTRAFAC_GTPase_membersPANTHER
MF_00118_AEF_Tu_ATransl_elong_EF1A_euk/arcHAMAP
TIGR00483EF-1_alphaTransl_elong_EF1A_euk/arcNCBIFAM
NF008969PRK12317.1NCBIFAM

Orthologs

References / Literature

PMID:31276588 - Inositol pyrophosphates impact phosphate homeostasis via modulation of RNA 3' processing and transcription termination.
Sanchez AM et al. Nucleic Acids Res 2019 Sep 19;47(16):8452-8469
GO_REF:0000002 - Comments
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:27168121 - Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. Yeast 2016 Sep;33(9):507-17
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:36820394 - Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast.
Sanchez AM et al. MicroPubl Biol 2023;2023
PMID:24634168 - Proteome-wide search for PP2A substrates in fission yeast.
Bernal M et al. Proteomics 2014 Jun;14(11):1367-80
PMID:9099890 - Comprehensive cloning of Schizosaccharomyces pombe genes encoding translation elongation factors.
Mita K et al. Gene 1997 Mar 18;187(2):259-66
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:28801462 - Protein glutaminylation is a yeast-specific posttranslational modification of elongation factor 1A.
Jank T et al. J Biol Chem 2017 Sep 29;292(39):16014-16023
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:36799444 - Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast.
Hirano Y et al. J Biochem 2023 Jun 30;174(1):33-46
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:36630955 - Zinc-finger protein Zpr1 is a bespoke chaperone essential for eEF1A biogenesis.
Sabbarini IM et al. Mol Cell 2023 Jan 19;83(2):252-265.e13
PMID:24818994 - The S. pombe translation initiation factor eIF4G is Sumoylated and associates with the SUMO protease Ulp2.
Jongjitwimol J et al. PLoS One 2014;9(5):e94182
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:10593605 - Cloning of a Schizosaccharomyces pombe homologue of elongation factor 1 alpha by two-hybrid selection of calmodulin-binding proteins.
Rasmussen C et al. Biochem Cell Biol 1999;77(5):421-30
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:18391219 - The conserved Wobble uridine tRNA thiolase Ctu1-Ctu2 is required to maintain genome integrity.
Dewez M et al. Proc Natl Acad Sci U S A 2008 Apr 08;105(14):5459-64
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:3214489 - Peptide elongation factor 1 from yeasts: purification and biochemical characterization of peptide elongation factors 1 alpha and 1 beta (gamma) from Saccharomyces carlsbergensis and Schizosaccharomyces pombe.
Miyazaki M et al. J Biochem 1988 Mar;103(3):508-21
PMID:10526238 - Overproduction of elongation factor 1alpha, an essential translational component, causes aberrant cell morphology by affecting the control of growth polarity in fission yeast.
Suda M et al. Genes Cells 1999 Sep;4(9):517-27
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:26115316 - Saccharomyces cerevisiae Eukaryotic Elongation Factor 1A (eEF1A) Is Methylated at Lys-390 by a METTL21-Like Methyltransferase.
Jakobsson ME et al. PLoS One 2015;10(6):e0131426