protein coding gene - frs1 (SPAC23A1.12c) - cytoplasmic phenylalanine-tRNA ligase beta subunit Frs1

Gene summary

Standard name: frs1
Systematic ID: SPAC23A1.12c
Product: cytoplasmic phenylalanine-tRNA ligase beta subunit Frs1
Organism: Schizosaccharomyces pombe (fission yeast)
Synonyms: frs2
UniProt ID: O42849
ORFeome ID: 36/36F08
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome I: 4098246..4100159 reverse strand

Annotation

Disease association

MONDO:0100215 - Rajab interstitial lung disease with brain calcifications 1

References:

PB_REF:0000006

GO biological process

GO:0002181 - cytoplasmic translation

References:

GO_REF:0000051

GO cellular component

GO:0005829 - cytosol

References:

PMID:16823372

GO:0005634 - nucleus

References:

PMID:16823372

GO:0009328 - phenylalanine-tRNA ligase complex

References:

GO_REF:0000033

GO molecular function

GO:0005524 - ATP binding

References:

GO_REF:0000002

GO:0000287 - magnesium ion binding

References:

GO_REF:0000024

GO:0004826 - phenylalanine-tRNA ligase activity

References:

GO_REF:0000033

GO:0003723 - RNA binding

References:

GO_REF:0000002

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

PMID:37970674

Multi-locus phenotype

FYPO:0000840 - normal RNA level

References:

PMID:29596413

Genotypes:

frs1-I406L(aa) trm7delta

FYPO:0006836 - sensitive to magnesium chloride

References:

PMID:41203586

Genotypes:

emp43delta frs1+(wild type)[Overexpression]

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PMID:39367033

PomGeneEx:0000027 - ribosomal density decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PMID:27984744

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0002151 - inviable spore

References:

PMID:23697806

Genotypes:

frs1delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

frs1delta

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF03483	B3_4	B3/B4_tRNA-bd	Pfam
PF03484	B5	tRNA_synthase_B5-dom	Pfam
PF17759	tRNA_synthFbeta	PheRS_beta_core	Pfam
PF18262	PhetRS_B1	PhetRS_B1	Pfam
cd00769	PheRS_beta_core	PheRS_beta_core	CDD
PS51483	B5	tRNA_synthase_B5-dom	PROSITE profiles
SM00873	B3_4	B3/B4_tRNA-bd	SMART
SM00874	B5	tRNA_synthase_B5-dom	SMART
G3DSA:3.30.56.10:FF:000006			CATH-FunFam
G3DSA:3.30.56.10:FF:000012			CATH-FunFam
G3DSA:3.30.930.10:FF:000059			CATH-FunFam
G3DSA:3.50.40.10:FF:000002			CATH-FunFam
G3DSA:3.30.56.10			CATH-Gene3D
G3DSA:3.30.930.10		aa-tRNA-synth_II/BPL/LPL	CATH-Gene3D
G3DSA:3.50.40.10		Phe-tRNA_synthase-like_B3/B4	CATH-Gene3D
SSF46955		DNA-bd_dom_put_sf	SUPERFAMILY
SSF55681		aa-tRNA-synth_II/BPL/LPL	SUPERFAMILY
PTHR10947		Phe-tRNA-ligase_IIc_bsu	PANTHER
TIGR00471	pheT_arch	Phe-tRNA-synth_IIc_bsu_arc_euk	NCBIFAM

Orthologs

FARSB (HGNC:17800) Homo sapiens
frs1 (SJAG_02206) Schizosaccharomyces japonicus
FRS1 (YLR060W) Saccharomyces cerevisiae

References / Literature

PMID:29596413 - Lack of 2'-O-methylation in the tRNA anticodon loop of two phylogenetically distant yeast species activates the general amino acid control pathway.: Han L et al. PLoS Genet 2018 Mar;14(3):e1007288
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.: Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:41203586 - Identification of an ERGIC-Like Compartment in Fission Yeast: Emp43 Functions as a Lectin-Like Cargo Receptor for Glycosylated Proteins.: Imamura I et al. Mol Microbiol 2025 Nov 07;
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.: Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.: Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.: Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.: Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.: Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.: Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000002 - Comments
GO_REF:0000051 - S. pombe keyword mapping
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.: Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.: Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.: Lee J et al. Nat Commun 2017 Feb 20;8:14527