PomBase home

protein coding gene - zhf1 (SPAC23C11.14) - ER zinc importer, CDF family, Zhf1

Gene summary

Standard name
zhf1
Systematic ID
SPAC23C11.14
Product
ER zinc importer, CDF family, Zhf1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
zhf
UniProt ID
O13918
ORFeome ID
18/18D05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2158307..2160086 forward strand

Annotation

GO biological process

GO:0010312 - detoxification of zinc ion

References:

GO:0006882 - intracellular zinc ion homeostasis

References:

GO:0140209 - zinc ion import into endoplasmic reticulum

References:

GO:0120127 - response to zinc ion starvation

References:

GO cellular component

GO:0005789 - endoplasmic reticulum membrane

References:

GO:0000329 - fungal-type vacuole membrane

References:

GO:0000329 - fungal-type vacuole membrane

References:

GO molecular function

GO:0140486 - zinc ion sequestering activity

References:

GO:0005385 - zinc ion transmembrane transporter activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0007042 - decreased alkaline phosphatase activity

References:

Genotypes:

Protein sequence feature

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000013 - RNA level unchanged

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0001550 - altered protein level during cellular response to zinc ion

References:

Genotypes:

FYPO:0001534 - decreased cellular zinc level

References:

Genotypes:

FYPO:0001546 - decreased RNA level during cellular response to zinc ion

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0007041 - increased alkaline phosphatase activity

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0006828 - increased cytosolic zinc level

References:

Genotypes:

FYPO:0001551 - increased protein level during cellular response to zinc ion starvation

References:

Genotypes:

FYPO:0001547 - increased RNA level during cellular response to zinc ion starvation

References:

Genotypes:

FYPO:0004557 - increased vegetative cell population growth

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0007047 - normal alkaline phosphatase activity

References:

Genotypes:

FYPO:0001199 - normal cellular calcium level

References:

Genotypes:

FYPO:0001536 - normal cellular copper level

References:

Genotypes:

FYPO:0001537 - normal cellular iron level

References:

Genotypes:

FYPO:0001539 - normal cellular magnesium level

References:

Genotypes:

FYPO:0001540 - normal cellular manganese level

References:

Genotypes:

FYPO:0001541 - normal cellular molybdenum level

References:

Genotypes:

FYPO:0001542 - normal cellular nickel level

References:

Genotypes:

FYPO:0001543 - normal cellular phosphorus level

References:

Genotypes:

FYPO:0001538 - normal cellular potassium level

References:

Genotypes:

FYPO:0001544 - normal cellular sulfur level

References:

Genotypes:

FYPO:0002619 - normal growth on sodium butyrate

References:

Genotypes:

FYPO:0002343 - normal growth on terbinafine

References:

Genotypes:

FYPO:0002620 - normal growth on trichostatin A

References:

Genotypes:

FYPO:0009030 - resistance to amitrole

References:

Genotypes:

FYPO:0009036 - resistance to benzamidine

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0001450 - resistance to cold

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0009070 - resistance to itraconazole

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0005969 - resistance to magnesium chloride

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002588 - resistance to nickel

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0002640 - sensitive to clotrimazole

References:

Genotypes:

FYPO:0001245 - sensitive to cobalt

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0000116 - sensitive to zinc

References:

Genotypes:

FYPO:0001533 - sensitive to zinc starvation

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF16916ZT_dimerCation_efflux_CTDPFAM
PF01545Cation_effluxCation_efflux_TMPFAM
SSF161111Cation efflux protein transmembrane domain-likeCation_efflux_TMD_sfSUPERFAMILY
SSF160240Cation efflux protein cytoplasmic domain-likeCation_efflux_CTD_sfSUPERFAMILY
G3DSA:1.20.1510.10Cation efflux protein transmembrane domainCation_efflux_TMD_sfGENE3D
PTHR45820FI23527P1PANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
TIGR01297CDFCation_effluxNCBIFAM

Orthologs

References / Literature

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:27053105 - Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast.
Burr R et al. J Biol Chem 2016 Jun 03;291(23):12171-83
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:29529046 - Zinc transporters belonging to the Cation Diffusion Facilitator (CDF) family have complementary roles in transporting zinc out of the cytosol.
Choi S et al. PLoS Genet 2018 Mar;14(3):e1007262
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:12050156 - Surplus zinc is handled by Zym1 metallothionein and Zhf endoplasmic reticulum transporter in Schizosaccharomyces pombe.
Borrelly GP et al. J Biol Chem 2002 Aug 16;277(33):30394-400
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23861937 - Genome-wide screening for genes associated with valproic acid sensitivity in fission yeast.
Zhang L et al. PLoS One 2013;8(7):e68738
PMID:11886869 - A transporter in the endoplasmic reticulum of Schizosaccharomyces pombe cells mediates zinc storage and differentially affects transition metal tolerance.
Clemens S et al. J Biol Chem 2002 May 17;277(20):18215-21
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:22806344 - Genome-wide screen reveals novel mechanisms for regulating cobalt uptake and detoxification in fission yeast.
Ryuko S et al. Mol Genet Genomics 2012 Aug;287(8):651-62
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:18637840 - Loss of Zhf and the tightly regulated zinc-uptake system SpZrt1 in Schizosaccharomyces pombe reveals the delicacy of cellular zinc balance.
Boch A et al. FEMS Yeast Res 2008 Sep;8(6):883-96
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:22252817 - A genomewide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis.
Fang Y et al. Antimicrob Agents Chemother 2012 Apr;56(4):1949-59
PMID:31239353 - Zinc-dependent activation of the Pho8 alkaline phosphatase in Schizosaccharomyces pombe .
Hu YM et al. J Biol Chem 2019 Aug 16;294(33):12392-12404
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.
Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36