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protein coding gene - les1 (SPAC23C4.05c) - nuclear bridge Ish domain protein Les1

Gene summary

Standard name
les1
Systematic ID
SPAC23C4.05c
Product
nuclear bridge Ish domain protein Les1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ish2
UniProt ID
O94559
ORFeome ID
20/20H06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1036639..1038209 reverse strand

Annotation

GO biological process

GO:0140515 - mitotic nuclear bridge organization

References:

GO:1905557 - regulation of mitotic nuclear envelope disassembly

References:

GO cellular component

GO:0140511 - mitotic nuclear bridge stalk

References:

GO:0005637 - nuclear inner membrane

References:

Modification

MOD:00006 - N-glycosylated residue

References:

Multi-locus phenotype

FYPO:0000337 - abnormal mitosis

References:

Genotypes:

FYPO:0003783 - abnormal nuclear pore localization during mitosis

References:

Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

References:

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0007459 - abnormal nuclear envelope morphology at mitotic nuclear bridge

References:

Genotypes:

FYPO:0003783 - abnormal nuclear pore localization during mitosis

References:

Genotypes:

FYPO:0007458 - abolished nuclear pore localization to mitotic nuclear bridge

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Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

References:

Genotypes:

FYPO:0007974 - increased rate of interpolar microtubule polymerization inside the mitotic nuclear membrane bridge

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0000772 - perforated nuclear envelope

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Genotypes:

FYPO:0006014 - promiscuous mating

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0006930 - sensitive to butylated hydroxyanisole

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Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF10281Ish1Ish1/Msc1-likePFAM

Orthologs

References / Literature

PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23231582 - Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.
Carter-O'Connell I et al. BMC Genomics 2012 Dec 12;13:697
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:35293864 - Microtubule rescue at midzone edges promotes overlap stability and prevents spindle collapse during anaphase B.
Lera-Ramirez M et al. Elife 2022 Mar 16;11
PMID:36043331 - Fission yeast Ish1 and Les1 interact with each other in the lumen of the nuclear envelope.
Asakawa H et al. Genes Cells 2022 Nov;27(11):643-656
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:32848252 - Closed mitosis requires local disassembly of the nuclear envelope.
Dey G et al. Nature 2020 Sep;585(7823):119-123
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531