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protein coding gene - rho3 (SPAC23C4.08) - Rho family GTPase Rho3

Gene summary

Standard name
rho3
Systematic ID
SPAC23C4.08
Product
Rho family GTPase Rho3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O13928
ORFeome ID
18/18D04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1041449..1043083 forward strand

Annotation

Disease association

MONDO:0032884 - ectodermal dysplasia with facial dysmorphism and acral, ocular, and brain anomalies

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GO biological process

GO:0030036 - actin cytoskeleton organization

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GO:0007163 - establishment or maintenance of cell polarity

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GO:0000226 - microtubule cytoskeleton organization

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GO:0032956 - regulation of actin cytoskeleton organization

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GO:0017157 - regulation of exocytosis

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GO:0010590 - regulation of septum digestion after cytokinesis

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GO:0007165 - signal transduction

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GO cellular component

GO:0032153 - cell division site

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GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO:0005886 - plasma membrane

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GO molecular function

GO:0005525 - GTP binding

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GO:0003924 - GTPase activity

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GO:0005515 - protein binding

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Modification

MOD:00437 - farnesylated residue

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MOD:00441 - geranylgeranylated residue

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MOD:00114 - L-cysteine methyl ester

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MOD:00440 - palmitoylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001370 - abnormal protein localization

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Genotypes:

FYPO:0000537 - abolished protein secretion during vegetative growth

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0000650 - increased septation index

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000118 - multiseptate vegetative cell

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0000079 - sensitive to caspofungin

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Genotypes:

FYPO:0002106 - viable stubby vegetative cell

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Genotypes:

Protein features

PBO:0111810 - Rho family

Qualitative gene expression

PomGeneEx:0000020 - protein level unchanged

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PomGeneEx:0000021 - protein present

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0002504 - abolished protein palmitoylation

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Genotypes:

FYPO:0009075 - decreased cell population growth on fructose carbon source

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Genotypes:

FYPO:0004572 - decreased exocytosis during vegetative growth

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Genotypes:

FYPO:0000470 - decreased mating type switching

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Genotypes:

FYPO:0001586 - decreased protein localization to cell tip during vegetative growth

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Genotypes:

FYPO:0000538 - decreased protein secretion during vegetative growth

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0002444 - loss of punctate cytoplasmic protein localization

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0002620 - normal growth on trichostatin A

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Genotypes:

FYPO:0000760 - normal mating

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Genotypes:

FYPO:0001903 - normal septation index

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Genotypes:

FYPO:0004795 - normal zygotic meiosis

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007514 - sensitive to manumycin A

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002617 - sensitive to sodium butyrate

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Warnings

PBO:0000070 - gene structure updated

Protein features

IDNameInterPro nameDB name
PF00071RasSmall_GTPasePFAM
cd04134Rho3CDD
PS51419RABPROSITE_PROFILES
PS51420RHOPROSITE_PROFILES
PS51421RASSmall_GTPasePROSITE_PROFILES
SM00175rab_sub_5SMART
SM00173ras_sub_4SMART
SM00174rho_sub_3Small_GTPaseSMART
SM00176ran_sub_2SMART
PR00449RASTRNSFRMNGPRINTS
G3DSA:3.40.50.300:FF:000780FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
PTHR24072RHO FAMILY GTPASESmall_GTPase_RhoPANTHER
TIGR00231small_GTPSmall_GTP-bdNCBIFAM

Orthologs

References / Literature

PMID:36650056 - Protein S-palmitoylation regulates different stages of meiosis in Schizosaccharomyces pombe .
Pham TV et al. Life Sci Alliance 2023 Apr;6(4)
PMID:25411334 - The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis.
Wang N et al. Mol Biol Cell 2015 Jan 15;26(2):238-55
PMID:25724972 - Rho4 interaction with exocyst and septins regulates cell separation in fission yeast.
Pérez P et al. Microbiology (Reading) 2015 May;161(Pt 5):948-959
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21304827 - Role of the Small GTPase Rho3 in Golgi/Endosome trafficking through functional interaction with adaptin in Fission Yeast.
Kita A et al. PLoS One 2011 Feb 03;6(2):e16842
PMID:21401840 - Pob1 ensures cylindrical cell shape by coupling two distinct rho signaling events during secretory vesicle targeting.
Nakano K et al. Traffic 2011 Jun;12(6):726-39
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21899677 - Cdc42 regulates multiple membrane traffic events in fission yeast.
Estravís M et al. Traffic 2011 Dec;12(12):1744-58
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:23840894 - Sip1, an AP-1 accessory protein in fission yeast, is required for localization of Rho3 GTPase.
Yu Y et al. PLoS One 2013;8(7):e68488
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:29290560 - ER-PM Contacts Restrict Exocytic Sites for Polarized Morphogenesis.
Ng AYE et al. Curr Biol 2018 Jan 08;28(1):146-153.e5
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23843742 - Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.
Zhang MM et al. PLoS Biol 2013 Jul;11(7):e1001597
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25422470 - Oscillatory AAA+ ATPase Knk1 constitutes a novel morphogenetic pathway in fission yeast.
Scheffler K et al. Proc Natl Acad Sci U S A 2014 Dec 16;111(50):17899-904
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:12930742 - Rho3p regulates cell separation by modulating exocyst function in Schizosaccharomyces pombe.
Wang H et al. Genetics 2003 Aug;164(4):1323-31
PMID:18793338 - Rga2 is a Rho2 GAP that regulates morphogenesis and cell integrity in S. pombe.
Villar-Tajadura MA et al. Mol Microbiol 2008 Nov;70(4):867-81
PMID:14637153 - Role of guanine nucleotide exchange factors for Rho family GTPases in the regulation of cell morphology and actin cytoskeleton in fission yeast.
Iwaki N et al. Biochem Biophys Res Commun 2003 Dec 12;312(2):414-20
PMID:10617635 - Protein farnesylation is critical for maintaining normal cell morphology and canavanine resistance in Schizosaccharomyces pombe.
Yang W et al. J Biol Chem 2000 Jan 07;275(1):429-38
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:24820419 - Rho2 palmitoylation is required for plasma membrane localization and proper signaling to the fission yeast cell integrity mitogen- activated protein kinase pathway.
Sánchez-Mir L et al. Mol Cell Biol 2014 Jul;34(14):2745-59
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:25500221 - Balance between exocytosis and endocytosis determines the efficacy of sterol-targeting antibiotics.
Nishimura S et al. Chem Biol 2014 Dec 18;21(12):1690-9
PMID:16415366 - A role for the septation initiation network in septum assembly revealed by genetic analysis of sid2-250 suppressors.
Jin QW et al. Genetics 2006 Apr;172(4):2101-12
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:31666919 - Rho Family GTPases in Fission Yeast Cytokinesis.
Hercyk B et al. Commun Integr Biol 2019;12(1):171-180
PMID:16421249 - Rgf1p is a specific Rho1-GEF that coordinates cell polarization with cell wall biogenesis in fission yeast.
García P et al. Mol Biol Cell 2006 Apr;17(4):1620-31
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:23934882 - Negative functional interaction between cell integrity MAPK pathway and Rho1 GTPase in fission yeast.
Viana RA et al. Genetics 2013 Oct;195(2):421-32
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:24947517 - The putative exchange factor Gef3p interacts with Rho3p GTPase and the septin ring during cytokinesis in fission yeast.
Muñoz S et al. J Biol Chem 2014 Aug 08;289(32):21995-2007
PB_REF:0000001 - Protein modification annotation by manual transfer of experimentally-verified annotation data to orthologs based on curator judgment of sequence features.
PMID:23861937 - Genome-wide screening for genes associated with valproic acid sensitivity in fission yeast.
Zhang L et al. PLoS One 2013;8(7):e68738
PMID:30044717 - Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis.
Zhu YH et al. Mol Biol Cell 2018 Sep 15;29(19):2259-2279
PMID:12415007 - The small GTPase Rho3 and the diaphanous/formin For3 function in polarized cell growth in fission yeast.
Nakano K et al. J Cell Sci 2002 Dec 01;115(Pt 23):4629-39
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs