protein coding gene - rpb5 (SPAC23C4.15) - DNA-directed RNA polymerase I, II and III subunit Rpb5

Gene summary

Standard name

rpb5

Systematic ID

SPAC23C4.15

Product

DNA-directed RNA polymerase I, II and III subunit Rpb5

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

Q09191

ORFeome ID

12/12E06

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 1054619..1055906 forward strand

Annotation

Protein features

Orthologs

• POLR2E (HGNC:9192) Homo sapiens
• rpb5 (SJAG_02101) Schizosaccharomyces japonicus
• RPB5 (YBR154C) Saccharomyces cerevisiae

References / Literature

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:9548938 - Location of subunit-subunit contact sites on RNA polymerase II subunit 3 from the fission yeast Schizosaccharomyces pombe.

Yasui K et al. Biochemistry 1998 Apr 21;37(16):5542-8

PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.

Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870

PMID:15743411 - Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation.

Hayashi K et al. Genes Cells 2005 Mar;10(3):207-24

PMID:9322754 - Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II.

Sakurai H et al. Gene 1997 Sep 01;196(1-2):165-74

PMID:9325316 - RNA polymerase II subunits 2, 3, and 11 form a core subassembly with DNA binding activity.

Kimura M et al. J Biol Chem 1997 Oct 10;272(41):25851-5

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:27401558 - The Nrd1-like protein Seb1 coordinates cotranscriptional 3' end processing and polyadenylation site selection.

Lemay JF et al. Genes Dev 2016 Jul 01;30(13):1558-72

PMID:15182371 - Mediator is required for activated transcription in a Schizosaccharomyces pombe in vitro system.

Tamayo E et al. Eur J Biochem 2004 Jun;271(12):2561-72

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:10648788 - Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II.

Kimura M et al. Nucleic Acids Res 2000 Feb 15;28(4):952-9

PMID:26792892 - Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast.

Suzuki S et al. Nucleic Acids Res 2016 May 19;44(9):4147-62

PMID:15620689 - Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit.

Mitsuzawa H et al. FEBS Lett 2005 Jan 03;579(1):48-52

PMID:10625684 - Purification and characterization of RNA polymerase II holoenzyme from Schizosaccharomyces pombe.

Spåhr H et al. J Biol Chem 2000 Jan 14;275(2):1351-6

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:11168400 - Intracellular contents and assembly states of all 12 subunits of the RNA polymerase II in the fission yeast Schizosaccharomyces pombe.

Kimura M et al. Eur J Biochem 2001 Feb;268(3):612-9

PMID:10653691 - Functional conservation of RNA polymerase II in fission and budding yeasts.

Shpakovski GV et al. J Mol Biol 2000 Feb 04;295(5):1119-27

PMID:11918671 - Level of the RNA polymerase II in the fission yeast stays constant but phosphorylation of its carboxyl terminal domain varies depending on the phase and rate of cell growth.

Sakurai H et al. Genes Cells 2002 Mar;7(3):273-84

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:19458260 - Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.

Spåhr H et al. Proc Natl Acad Sci U S A 2009 Jun 09;106(23):9185-90

PMID:33771877 - RNA polymerase backtracking results in the accumulation of fission yeast condensin at active genes.

Rivosecchi J et al. Life Sci Alliance 2021 Jun;4(6)

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:30282034 - Elongation/Termination Factor Exchange Mediated by PP1 Phosphatase Orchestrates Transcription Termination.

Kecman T et al. Cell Rep 2018 Oct 02;25(1):259-269.e5

PMID:9499374 - [Molecular cloning of rpb5+, rpb7+ and rpb11+ genes of the fission yeast Schizosaccharomyces pombe: completing primary structure of all indispensable subunits of its RNA polymerase II].

Shpakovskiĭ GV et al. Bioorg Khim 1997 Dec;23(12):988-91

PMID:9642054 - Two large subunits of the fission yeast RNA polymerase II provide platforms for the assembly of small subunits.

Ishiguro A et al. J Mol Biol 1998 Jun 19;279(4):703-12

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:10503538 - Isolation and characterization of temperature-sensitive mutations in the gene (rpb3) for subunit 3 of RNA polymerase II in the fission yeast Schizosaccharomyces pombe.

Mitobe J et al. Mol Gen Genet 1999 Aug;262(1):73-84

GO_REF:0000002 - Comments

PMID:11839823 - Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II.

Kimura M et al. Mol Cell Biol 2002 Mar;22(5):1577-88

PMID:28476936 - Genome-wide screen for cell growth regulators in fission yeast.

Weston L et al. J Cell Sci 2017 Jun 15;130(12):2049-2055

PMID:31883795 - Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.

Holla S et al. Cell 2020 Jan 09;180(1):150-164.e15

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.

Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PMID:24583182 - A novel method for purification of the endogenously expressed fission yeast Set2 complex.

Suzuki S et al. Protein Expr Purif 2014 May;97:44-9

PMID:16204182 - RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing.

Djupedal I et al. Genes Dev 2005 Oct 01;19(19):2301-6

PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902

PMID:28241144 - Mediator structure and rearrangements required for holoenzyme formation.

Tsai KL et al. Nature 2017 Apr 13;544(7649):196-201

PMID:33536435 - Conserved strategies of RNA polymerase I hibernation and activation.

Heiss FB et al. Nat Commun 2021 Feb 03;12(1):758

PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.

Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003

PMID:39746995 - DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts.

Kuś K et al. Nat Commun 2025 Jan 02;16(1):10

PMID:9077438 - Molecular assembly of RNA polymerase II from the fission yeast Schizosaccharomyces pombe: subunit-subunit contact network involving Rpb5.

Miyao T et al. Genes Cells 1996 Sep;1(9):843-54

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.

Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:9738888 - Mapping of Rpb3 and Rpb5 contact sites on two large subunits, Rpb1 and Rpb2, of the RNA polymerase II from fission yeast.

Miyao T et al. Mol Gen Genet 1998 Jul;259(1):123-9