protein coding gene - fep1 (SPAC23E2.01) - DNA-binding transcription factor, zf-GATA type, iron-sensing Fep1

Gene summary

Standard name

fep1

Systematic ID

SPAC23E2.01

Product

DNA-binding transcription factor, zf-GATA type, iron-sensing Fep1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

gaf2

UniProt ID

Q10134

ORFeome ID

35/35C10

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 442221..445721 forward strand

Annotation

Protein features

Orthologs

• fep1 (SJAG_00792) Schizosaccharomyces japonicus
• GZF3 (YJL110C) Saccharomyces cerevisiae
• DAL80 (YKR034W) Saccharomyces cerevisiae

References / Literature

PMID:28882432 - Analysis of ambient pH stress response mediated by iron and copper intake in Schizosaccharomyces pombe.

Higuchi Y et al. J Biosci Bioeng 2018 Jan;125(1):92-96

PMID:22540037 - Predicting the fission yeast protein interaction network.

Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.

Mojardín L et al. Cell Cycle 2015;14(2):206-18

PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.

Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.

Blyth J et al. Genetics 2018 Feb;208(2):589-603

PMID:17724773 - Expression of Candida albicans Sfu1 in fission yeast complements the loss of the iron-regulatory transcription factor Fep1 and requires Tup co-repressors.

Pelletier B et al. Yeast 2007 Oct;24(10):883-900

PMID:18665268 - Mitochondrial dysfunction increases oxidative stress and decreases chronological life span in fission yeast.

Zuin A et al. PLoS One 2008 Jul 30;3(7):e2842

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:21531205 - Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast.

Kim KD et al. Biochem Biophys Res Commun 2011 May 20;408(4):609-14

PMID:25806539 - A cascade of iron-containing proteins governs the genetic iron starvation response to promote iron uptake and inhibit iron storage in fission yeast.

Encinar del Dedo J et al. PLoS Genet 2015 Mar;11(3):e1005106

PMID:29549126 - The major facilitator transporter Str3 is required for low-affinity heme acquisition in Schizosaccharomyces pombe .

Normant V et al. J Biol Chem 2018 Apr 27;293(17):6349-6362

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:18223116 - Key function for the CCAAT-binding factor Php4 to regulate gene expression in response to iron deficiency in fission yeast.

Mercier A et al. Eukaryot Cell 2008 Mar;7(3):493-508

PMID:11956219 - Fep1, an iron sensor regulating iron transporter gene expression in Schizosaccharomyces pombe.

Pelletier B et al. J Biol Chem 2002 Jun 21;277(25):22950-8

PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.

Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643

PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.

Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.

Mak T et al. EMBO J 2021 Aug 16;40(16):e107911

PMID:14668334 - The Schizosaccharomyces pombe corepressor Tup11 interacts with the iron-responsive transcription factor Fep1.

Znaidi S et al. J Biol Chem 2004 Mar 05;279(10):9462-74

PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.

Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72

PMID:23695302 - Functional characterization of fission yeast transcription factors by overexpression analysis.

Vachon L et al. Genetics 2013 Aug;194(4):873-84

PMID:20435771 - Iron-dependent remodeling of fungal metabolic pathways associated with ferrichrome biosynthesis.

Mercier A et al. Appl Environ Microbiol 2010 Jun;76(12):3806-17

PMID:19915076 - abc3+ encodes an iron-regulated vacuolar ABC-type transporter in Schizosaccharomyces pombe.

Pouliot B et al. Eukaryot Cell 2010 Jan;9(1):59-73

PMID:21421748 - Grx4 monothiol glutaredoxin is required for iron limitation-dependent inhibition of Fep1.

Jbel M et al. Eukaryot Cell 2011 May;10(5):629-45

PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.

Rallis C et al. Sci Rep 2017 Mar 10;7:44257

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:29686279 - Iron homeostasis regulates facultative heterochromatin assembly in adaptive genome control.

Gallagher PS et al. Nat Struct Mol Biol 2018 May;25(5):372-383

PMID:30148840 - Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks.

Ganguly A et al. PLoS Genet 2018 Aug;14(8):e1007595

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:17477863 - Global transcriptional responses of fission and budding yeast to changes in copper and iron levels: a comparative study.

Rustici G et al. Genome Biol 2007;8(5):R73

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:35924983 - Genetic-interaction screens uncover novel biological roles and regulators of transcription factors in fission yeast.

Chatfield-Reed K et al. G3 (Bethesda) 2022 Aug 25;12(9)

PMID:16963626 - A transcription factor cascade involving Fep1 and the CCAAT-binding factor Php4 regulates gene expression in response to iron deficiency in the fission yeast Schizosaccharomyces pombe.

Mercier A et al. Eukaryot Cell 2006 Nov;5(11):1866-81

PMID:25733668 - Shu1 is a cell-surface protein involved in iron acquisition from heme in Schizosaccharomyces pombe.

Mourer T et al. J Biol Chem 2015 Apr 17;290(16):10176-90

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:37923140 - Iron homeostasis proteins Grx4 and Fra2 control activity of the Schizosaccharomyces pombe iron repressor Fep1 by facilitating [2Fe-2S] cluster removal.

Hati D et al. J Biol Chem 2023 Nov 03;299(12):105419

PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.

Anver S et al. EMBO Rep 2014 Aug;15(8):894-902

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:19620282 - The fission yeast HIRA histone chaperone is required for promoter silencing and the suppression of cryptic antisense transcripts.

Anderson HE et al. Mol Cell Biol 2009 Sep;29(18):5158-67

PMID:24897379 - Fra2 is a co-regulator of Fep1 inhibition in response to iron starvation.

Jacques JF et al. PLoS One 2014;9(6):e98959

PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.

Køhler JB et al. Nat Commun 2015 Nov 05;6:8827

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:15866870 - Functional characterization of the iron-regulatory transcription factor Fep1 from Schizosaccharomyces pombe.

Pelletier B et al. J Biol Chem 2005 Jul 01;280(26):25146-61

PMID:26152587 - TORC1 Regulates Developmental Responses to Nitrogen Stress via Regulation of the GATA Transcription Factor Gaf1.

Laor D et al. mBio 2015 Jul 07;6(4):e00959

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.

Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.

Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480

PMID:23115244 - Cells lacking pfh1, a fission yeast homolog of mammalian frataxin protein, display constitutive activation of the iron starvation response.

Gabrielli N et al. J Biol Chem 2012 Dec 14;287(51):43042-51

PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.

Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721

PMID:25795664 - Genetic Interaction Landscape Reveals Critical Requirements for Schizosaccharomyces pombe Brc1 in DNA Damage Response Mutants.

Sánchez A et al. G3 (Bethesda) 2015 Mar 19;5(5):953-62

PMID:36568394 - Schizosaccharomyces pombe Grx4, Fep1, and Php4: In silico analysis and expression response to different iron concentrations.

Ebrahim A et al. Front Genet 2022;13:1069068

PMID:8799335 - Molecular cloning of GAF2, a Schizosaccharomyces pombe GATA factor, which has two zinc-finger sequences.

Hoe KL et al. Biochem Mol Biol Int 1996 May;39(1):127-35

PMID:27444384 - The iron uptake repressor Fep1 in the fission yeast binds Fe-S cluster through conserved cysteines.

Kim HJ et al. Biochem Biophys Res Commun 2016 Sep 09;478(1):187-192

PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.

Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8

PMID:12888492 - Fep1 represses expression of the fission yeast Schizosaccharomyces pombe siderophore-iron transport system.

Pelletier B et al. Nucleic Acids Res 2003 Aug 01;31(15):4332-44

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895