protein coding gene - rpb2 (SPAC23G3.01) - DNA-directed RNA polymerase II complex subunit Rpb2

Gene summary

Standard name

rpb2

Systematic ID

SPAC23G3.01

Product

DNA-directed RNA polymerase II complex subunit Rpb2

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

SPAC521.06

UniProt ID

Q02061

ORFeome ID

39/39B12

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 850636..854385 forward strand

Annotation

Protein features

Orthologs

• POLR2B (HGNC:9188) Homo sapiens
• rpb2 (SJAG_04030) Schizosaccharomyces japonicus
• RPB2 (YOR151C) Saccharomyces cerevisiae

References / Literature

PMID:15620689 - Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit.

Mitsuzawa H et al. FEBS Lett 2005 Jan 03;579(1):48-52

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:17948055 - The JmjC domain protein Epe1 prevents unregulated assembly and disassembly of heterochromatin.

Trewick SC et al. EMBO J 2007 Nov 14;26(22):4670-82

PMID:26792892 - Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast.

Suzuki S et al. Nucleic Acids Res 2016 May 19;44(9):4147-62

PMID:41316862 - Deletion of Elongator Protein 1 (Elp1) relieves heterochromatin defects in a Pol II mutant of Schizosaccharomyces pombe.

Nirmal MB et al. Genetics 2025 Nov 29;

PMID:22540037 - Predicting the fission yeast protein interaction network.

Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67

PMID:9325316 - RNA polymerase II subunits 2, 3, and 11 form a core subassembly with DNA binding activity.

Kimura M et al. J Biol Chem 1997 Oct 10;272(41):25851-5

PMID:19458260 - Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.

Spåhr H et al. Proc Natl Acad Sci U S A 2009 Jun 09;106(23):9185-90

PMID:9738888 - Mapping of Rpb3 and Rpb5 contact sites on two large subunits, Rpb1 and Rpb2, of the RNA polymerase II from fission yeast.

Miyao T et al. Mol Gen Genet 1998 Jul;259(1):123-9

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:30282034 - Elongation/Termination Factor Exchange Mediated by PP1 Phosphatase Orchestrates Transcription Termination.

Kecman T et al. Cell Rep 2018 Oct 02;25(1):259-269.e5

PMID:9988759 - Functional organization of two large subunits of the fission yeast Schizosaccharomyces pombe RNA polymerase II. Location of the catalytic sites.

Wlassoff WA et al. J Biol Chem 1999 Feb 19;274(8):5104-13

PMID:10625684 - Purification and characterization of RNA polymerase II holoenzyme from Schizosaccharomyces pombe.

Spåhr H et al. J Biol Chem 2000 Jan 14;275(2):1351-6

PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.

Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7

PMID:9322754 - Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II.

Sakurai H et al. Gene 1997 Sep 01;196(1-2):165-74

PMID:11168400 - Intracellular contents and assembly states of all 12 subunits of the RNA polymerase II in the fission yeast Schizosaccharomyces pombe.

Kimura M et al. Eur J Biochem 2001 Feb;268(3):612-9

PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.

Li J et al. Nat Commun 2019 Mar 01;10(1):1002

PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.

Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:32059768 - Size-Dependent Increase in RNA Polymerase II Initiation Rates Mediates Gene Expression Scaling with Cell Size.

Sun XM et al. Curr Biol 2020 Apr 06;30(7):1217-1230.e7

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.

Nie M et al. Sci Rep 2015 Sep 25;5:14389

PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.

Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671

PMID:10648788 - Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II.

Kimura M et al. Nucleic Acids Res 2000 Feb 15;28(4):952-9

PMID:8441660 - Cloning and sequence determination of the Schizosaccharomyces pombe rpb2 gene encoding the subunit 2 of RNA polymerase II.

Kawagishi M et al. Nucleic Acids Res 1993 Feb 11;21(3):469-73

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PMID:39746995 - DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts.

Kuś K et al. Nat Commun 2025 Jan 02;16(1):10

PMID:11839823 - Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II.

Kimura M et al. Mol Cell Biol 2002 Mar;22(5):1577-88

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:11918671 - Level of the RNA polymerase II in the fission yeast stays constant but phosphorylation of its carboxyl terminal domain varies depending on the phase and rate of cell growth.

Sakurai H et al. Genes Cells 2002 Mar;7(3):273-84

PMID:12738880 - TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution.

Samuelsen CO et al. Proc Natl Acad Sci U S A 2003 May 27;100(11):6422-7

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:15798214 - A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.

Kizer KO et al. Mol Cell Biol 2005 Apr;25(8):3305-16

PMID:10503538 - Isolation and characterization of temperature-sensitive mutations in the gene (rpb3) for subunit 3 of RNA polymerase II in the fission yeast Schizosaccharomyces pombe.

Mitobe J et al. Mol Gen Genet 1999 Aug;262(1):73-84

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:15743411 - Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation.

Hayashi K et al. Genes Cells 2005 Mar;10(3):207-24

GO_REF:0000043 - Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

PMID:40132111 - A fission yeast CENP-B homologue Abp1 prevents RNAi-mediated heterochromatin formation at ribosomal DNA repeats.

Tsunemine S et al. Genetics 2025 Mar 25;

PMID:9077438 - Molecular assembly of RNA polymerase II from the fission yeast Schizosaccharomyces pombe: subunit-subunit contact network involving Rpb5.

Miyao T et al. Genes Cells 1996 Sep;1(9):843-54

GO_REF:0000002 - Comments

PMID:12552808 - [Mapping the interaction site of Rpb2 and Rpb3 subunit of fission yeast RNA polymerase II].

Qu Z et al. Wei Sheng Wu Xue Bao 2001 Oct;41(5):592-7

PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902

PMID:27401558 - The Nrd1-like protein Seb1 coordinates cotranscriptional 3' end processing and polyadenylation site selection.

Lemay JF et al. Genes Dev 2016 Jul 01;30(13):1558-72

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:9642054 - Two large subunits of the fission yeast RNA polymerase II provide platforms for the assembly of small subunits.

Ishiguro A et al. J Mol Biol 1998 Jun 19;279(4):703-12

PMID:28241144 - Mediator structure and rearrangements required for holoenzyme formation.

Tsai KL et al. Nature 2017 Apr 13;544(7649):196-201

PMID:24583182 - A novel method for purification of the endogenously expressed fission yeast Set2 complex.

Suzuki S et al. Protein Expr Purif 2014 May;97:44-9

PMID:8569679 - Mutational analysis of the RNase-like domain in subunit 2 of fission yeast RNA polymerase II.

Kawagishi-Kobayashi M et al. Mol Gen Genet 1996 Jan 15;250(1):1-6

PMID:16204182 - RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing.

Djupedal I et al. Genes Dev 2005 Oct 01;19(19):2301-6

PMID:15182371 - Mediator is required for activated transcription in a Schizosaccharomyces pombe in vitro system.

Tamayo E et al. Eur J Biochem 2004 Jun;271(12):2561-72

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:26889830 - A Novel Epigenetic Silencing Pathway Involving the Highly Conserved 5'-3' Exoribonuclease Dhp1/Rat1/Xrn2 in Schizosaccharomyces pombe.

Tucker JF et al. PLoS Genet 2016 Feb;12(2):e1005873

PMID:28476936 - Genome-wide screen for cell growth regulators in fission yeast.

Weston L et al. J Cell Sci 2017 Jun 15;130(12):2049-2055

PMID:15947136 - RNA polymerase II is required for RNAi-dependent heterochromatin assembly.

Kato H et al. Science 2005 Jul 15;309(5733):467-9

PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.

Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86