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protein coding gene - npr2 (SPAC23H3.03c) - GATOR1-SEACIT GTPase activating complex subunit Npr2

Gene summary

Standard name
npr2
Systematic ID
SPAC23H3.03c
Product
GATOR1-SEACIT GTPase activating complex subunit Npr2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O42857
ORFeome ID
22/22H04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2495844..2497562 reverse strand

Annotation

Disease association

MONDO:0014924 - epilepsy, familial focal, with variable foci 2

References:

GO biological process

GO:0034198 - cellular response to amino acid starvation

References:

GO:1904262 - negative regulation of TORC1 signaling

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:1990130 - GATOR1 complex

References:

GO:0005634 - nucleus

References:

GO:0005774 - vacuolar membrane

References:

GO molecular function

GO:0005096 - GTPase activator activity

References:

Multi-locus phenotype

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0004457 - decreased protein localization to nucleus during nitrogen starvation

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001029 - resistance to canavanine

References:

Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0002128 - abolished protein localization to plasma membrane, with protein mislocalized to cytoplasm, during vegetative growth

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0006148 - abolished transcription during nitrogen starvation

References:

Genotypes:

FYPO:0001575 - abolished vegetative cell population growth

References:

Genotypes:

FYPO:0000046 - decreased cell population growth

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0001176 - decreased cell population growth on sucrose carbon source

References:

Genotypes:

FYPO:0001140 - decreased leucine import

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0010009 - decreased protein localization to cell surface, with protein mislocalized to cytoplasm

References:

Genotypes:

FYPO:0004457 - decreased protein localization to nucleus during nitrogen starvation

References:

Genotypes:

FYPO:0006233 - decreased protein localization to vacuolar membrane

References:

Genotypes:

FYPO:0001152 - decreased RNA level during nitrogen starvation

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0002681 - increased protein phosphorylation during nitrogen starvation

References:

Genotypes:

FYPO:0001573 - increased RNA level during cellular response to rapamycin

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0004557 - increased vegetative cell population growth

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0001522 - normal growth on caffeine

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Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

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Genotypes:

FYPO:0001545 - normal growth on L-canavanine

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Genotypes:

FYPO:0002672 - normal growth on rapamycin

References:

Genotypes:

FYPO:0008372 - normal protein localization to cell surface during vegetative growth

References:

Genotypes:

FYPO:0002674 - normal protein localization to plasma membrane

References:

Genotypes:

FYPO:0004248 - normal protein localization to vacuolar membrane

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000783 - protein mislocalized to cytoplasm during vegetative growth

References:

Genotypes:

FYPO:0009030 - resistance to amitrole

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

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Genotypes:

FYPO:0009032 - resistance to bortezomib

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0001029 - resistance to canavanine

References:

Genotypes:

FYPO:0001450 - resistance to cold

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0009070 - resistance to itraconazole

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Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

References:

Genotypes:

FYPO:0009081 - resistance to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000086 - sensitive to tacrolimus

References:

Genotypes:

FYPO:0007938 - sensitive to tea tree oil

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0000281 - small vacuoles present in increased numbers during vegetative growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF06218NPR2NPR2-likePFAM
PTHR12991NITROGEN PERMEASE REGULATOR 2/TUMOR SUPPRESSOR CANDIDATE 4NPR2-likePANTHER

Orthologs

References / Literature

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23934889 - TORC1 signaling is governed by two negative regulators in fission yeast.
Ma N et al. Genetics 2013 Oct;195(2):457-68
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:33534698 - Tripartite suppression of fission yeast TORC1 signaling by the GATOR1-Sea3 complex, the TSC complex, and Gcn2 kinase.
Fukuda T et al. Elife 2021 Feb 03;10
PMID:34028542 - TORC2 inhibition of α-arrestin Aly3 mediates cell surface persistence of S. pombe Ght5 glucose transporter in low glucose.
Toyoda Y et al. J Cell Sci 2021 May 15;134(10)
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:38051102 - Fission Yeast TORC1 Promotes Cell Proliferation through Sfp1, a Transcription Factor Involved in Ribosome Biogenesis.
Tai YT et al. Mol Cell Biol 2023 Dec 05;:1-18
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:29199950 - Ragulator and GATOR1 complexes promote fission yeast growth by attenuating TOR complex 1 through Rag GTPases.
Chia KH et al. Elife 2017 Dec 04;6
PMID:21850271 - Genome-wide screening for genes associated with FK506 sensitivity in fission yeast.
Ma Y et al. PLoS One 2011;6(8):e23422
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:27227887 - The Loss of Lam2 and Npr2-Npr3 Diminishes the Vacuolar Localization of Gtr1-Gtr2 and Disinhibits TORC1 Activity in Fission Yeast.
Ma N et al. PLoS One 2016;11(5):e0156239
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:26098123 - Promoter nucleosome dynamics regulated by signalling through the CTD code.
Materne P et al. Elife 2015 Jun 22;4:e09008
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26689777 - Tor Signaling Regulates Transcription of Amino Acid Permeases through a GATA Transcription Factor Gaf1 in Fission Yeast.
Ma Y et al. PLoS One 2015;10(12):e0144677
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7