protein coding gene - bub3 (SPAC23H3.08c) - mitotic spindle checkpoint WD repeat protein Bub3

Gene summary

Standard name

bub3

Systematic ID

SPAC23H3.08c

Product

mitotic spindle checkpoint WD repeat protein Bub3

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

O42860

ORFeome ID

14/14H04

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 2506085..2507369 reverse strand

Annotation

Protein features

Orthologs

• BUB3 (HGNC:1151) Homo sapiens
• bub3 (SJAG_02451) Schizosaccharomyces japonicus
• BUB3 (YOR026W) Saccharomyces cerevisiae

References / Literature

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:26900649 - The hairpin region of Ndc80 is important for the kinetochore recruitment of Mph1/MPS1 in fission yeast.

Chmielewska AE et al. Cell Cycle 2016;15(5):740-7

PMID:28178520 - Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast.

Meadows JC et al. Cell Rep 2017 Feb 07;18(6):1422-1433

PMID:28366743 - Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex.

Sewart K et al. Curr Biol 2017 Apr 24;27(8):1213-1220

PMID:29440310 - Asp1 Bifunctional Activity Modulates Spindle Function via Controlling Cellular Inositol Pyrophosphate Levels in Schizosaccharomyces pombe.

Pascual-Ortiz M et al. Mol Cell Biol 2018 May 01;38(9)

PMID:25253718 - csi2p modulates microtubule dynamics and organizes the bipolar spindle for chromosome segregation.

Costa J et al. Mol Biol Cell 2014 Dec 01;25(24):3900-8

PMID:27618268 - Bub3-Bub1 Binding to Spc7/KNL1 Toggles the Spindle Checkpoint Switch by Licensing the Interaction of Bub1 with Mad1-Mad2.

Mora-Santos MD et al. Curr Biol 2016 Oct 10;26(19):2642-2650

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:24477934 - Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores.

Heinrich S et al. EMBO Rep 2014 Mar;15(3):291-8

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:31257143 - The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest.

Leontiou I et al. Curr Biol 2019 Jul 22;29(14):2407-2414.e7

PMID:16360688 - Control of Shugoshin function during fission-yeast meiosis.

Vaur S et al. Curr Biol 2005 Dec 20;15(24):2263-70

PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Han TX et al. Genome Biol 2010;11(6):R60

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:24161933 - Determinants of robustness in spindle assembly checkpoint signalling.

Heinrich S et al. Nat Cell Biol 2013 Nov;15(11):1328-39

PMID:28017606 - Generation of a Spindle Checkpoint Arrest from Synthetic Signaling Assemblies.

Yuan I et al. Curr Biol 2017 Jan 09;27(1):137-143

PMID:11909965 - Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and Mph1p-dependent manner.

Millband DN et al. Mol Cell Biol 2002 Apr;22(8):2728-42

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:15509783 - Kinetochore targeting of fission yeast Mad and Bub proteins is essential for spindle checkpoint function but not for all chromosome segregation roles of Bub1p.

Vanoosthuyse V et al. Mol Cell Biol 2004 Nov;24(22):9786-801

PMID:16394105 - The V260I mutation in fission yeast alpha-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint.

Asakawa K et al. Mol Biol Cell 2006 Mar;17(3):1421-35

PMID:22521786 - Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint.

Shepperd LA et al. Curr Biol 2012 May 22;22(10):891-9

PMID:28497540 - Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I.

Miyazaki S et al. Genes Cells 2017 Jun;22(6):552-567

PMID:21664573 - Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors.

Meadows JC et al. Dev Cell 2011 Jun 14;20(6):739-50

PMID:26258632 - Mad1 promotes chromosome congression by anchoring a kinesin motor to the kinetochore.

Akera T et al. Nat Cell Biol 2015 Sep;17(9):1124-33

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:12719471 - Sim4: a novel fission yeast kinetochore protein required for centromeric silencing and chromosome segregation.

Pidoux AL et al. J Cell Biol 2003 Apr 28;161(2):295-307

PMID:22184248 - Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1.

Ito D et al. Proc Natl Acad Sci U S A 2012 Jan 03;109(1):209-14

PMID:22825872 - Mph1 kinetochore localization is crucial and upstream in the hierarchy of spindle assembly checkpoint protein recruitment to kinetochores.

Heinrich S et al. J Cell Sci 2012 Oct 15;125(Pt 20):4720-7

PMID:15525673 - The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function.

Kadura S et al. Mol Biol Cell 2005 Jan;16(1):385-95

PMID:16179942 - Mal3, the fission yeast EB1 homologue, cooperates with Bub1 spindle checkpoint to prevent monopolar attachment.

Asakawa K et al. EMBO Rep 2005 Dec;6(12):1194-200

PMID:18204818 - Identification of small molecules inducing apoptosis by cell-based assay using fission yeast deletion mutants.

Chung KS et al. Invest New Drugs 2008 Aug;26(4):299-307

PMID:19846658 - Bub3p facilitates spindle checkpoint silencing in fission yeast.

Vanoosthuyse V et al. Mol Biol Cell 2009 Dec;20(24):5096-105

PMID:18562692 - Latrunculin A delays anaphase onset in fission yeast by disrupting an Ase1-independent pathway controlling mitotic spindle stability.

Meadows JC et al. Mol Biol Cell 2008 Sep;19(9):3713-23

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:22437499 - Structure of the mitotic checkpoint complex.

Chao WC et al. Nature 2012 Mar 21;484(7393):208-13

PMID:22660415 - MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components.

Yamagishi Y et al. Nat Cell Biol 2012 Jun 03;14(7):746-52

PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.

Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86

PMID:19680287 - Bub1 and Bub3 promote the conversion from monopolar to bipolar chromosome attachment independently of shugoshin.

Windecker H et al. EMBO Rep 2009 Sep;10(9):1022-8

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:25472718 - Alp7/TACC recruits kinesin-8-PP1 to the Ndc80 kinetochore protein for timely mitotic progression and chromosome movement.

Tang NH et al. J Cell Sci 2015 Jan 15;128(2):354-63

PMID:15483052 - BAF53/Arp4 homolog Alp5 in fission yeast is required for histone H4 acetylation, kinetochore-spindle attachment, and gene silencing at centromere.

Minoda A et al. Mol Biol Cell 2005 Jan;16(1):316-27

PMID:24583014 - Slow checkpoint activation kinetics as a safety device in anaphase.

Kamenz J et al. Curr Biol 2014 Mar 17;24(6):646-51