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protein coding gene - gln1 (SPAC23H4.06) - glutamate-ammonia ligase Gln1

Gene summary

Standard name
gln1
Systematic ID
SPAC23H4.06
Product
glutamate-ammonia ligase Gln1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
gog1
UniProt ID
Q09179
ORFeome ID
16/16G03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1596073..1598064 reverse strand

Annotation

Disease association

MONDO:0012393 - congenital brain dysgenesis due to glutamine synthetase deficiency

References:

MONDO:0970945 - developmental and epileptic encephalopathy 116

References:

GO biological process

GO:0019676 - ammonia assimilation cycle

References:

GO:1901704 - L-glutamine biosynthetic process

References:

GO cellular component

GO:0005829 - cytosol

References:

GO molecular function

GO:0004356 - glutamine synthetase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0005471 - abolished glutamate synthase (NADH) activity

References:

Genotypes:

FYPO:0002268 - abolished glutamate-ammonia ligase activity

References:

Genotypes:

FYPO:0002269 - decreased glutamate-ammonia ligase activity

References:

Genotypes:

FYPO:0002271 - growth auxotrophic for glutamine

References:

Genotypes:

FYPO:0002270 - increased glutamate-ammonia ligase activity

References:

Genotypes:

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0005474 - normal glutamate dehydrogenase (NADP+) activity

References:

Genotypes:

FYPO:0005473 - normal glutamate synthase (NADH) activity

References:

Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00120Gln-synt_CGln_synth_cat_domPFAM
PF03951Gln-synt_NGln_synt_NPFAM
PS00181GLNA_ATPGln_synth_gly_rich_sitePROSITE_PATTERNS
PS00180GLNA_1Gln_synth_N_conserv_sitePROSITE_PATTERNS
PS51986GS_BETA_GRASPGln_synt_NPROSITE_PROFILES
PS51987GS_CATALYTICGln_synth_cat_domPROSITE_PROFILES
SM01230Gln_synt_C_2Gln_synth_cat_domSMART
G3DSA:3.30.590.10:FF:000004FUNFAM
G3DSA:3.10.20.70:FF:000004FUNFAM
SSF55931Glutamine synthetase/guanido kinaseGln_synth/guanido_kin_cat_domSUPERFAMILY
SSF54368Glutamine synthetase, N-terminal domainGln_synt_N_sfSUPERFAMILY
G3DSA:3.10.20.70Gln_synt_N_sfGENE3D
G3DSA:3.30.590.10Glutamine synthetase/guanido kinase, catalytic domainGENE3D
PTHR20852GLUTAMINE SYNTHETASEGlutamine_SynthetasePANTHER

Orthologs

References / Literature

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:13760 - Ammonia assimilation in the fission yeast Schizosaccharomyces pombe 972.
van Andel JG et al. Arch Microbiol 1977 Jan 11;111(3):265-70
PMID:20435771 - Iron-dependent remodeling of fungal metabolic pathways associated with ferrichrome biosynthesis.
Mercier A et al. Appl Environ Microbiol 2010 Jun;76(12):3806-17
PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.
Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .
Budden AM et al. Elife 2023 Aug 24;12
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:2900077 - Isolation of a DNA fragment which complements glutamine synthetase deficient strains of S. pombe.
Barel I et al. Curr Genet 1988 Jun;13(6):487-94
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:24936793 - Fusion of protein aggregates facilitates asymmetric damage segregation.
Coelho M et al. PLoS Biol 2014 Jun;12(6):e1001886
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:28982178 - Filamentous invasive growth of mutants of the genes encoding ammonia-metabolizing enzymes in the fission yeast Schizosaccharomyces pombe.
Sasaki Y et al. PLoS One 2017;12(10):e0186028
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:7773104 - Glutamine synthetase/glutamate synthase ammonium-assimilating pathway in Schizosaccharomyces pombe.
Perysinakis A et al. Curr Microbiol 1995 Jun;30(6):367-72
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29