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protein coding gene - rbx1 (SPAC23H4.18c) - SCF complex, Cul4-RING and CLRC ubiquitin ligase ligase E3 subunit Rbx1

Gene summary

Standard name
rbx1
Systematic ID
SPAC23H4.18c
Product
SCF complex, Cul4-RING and CLRC ubiquitin ligase ligase E3 subunit Rbx1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
pip1
UniProt ID
O13959
ORFeome ID
42/42E02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 1575345..1576307 forward strand

Annotation

GO biological process

GO:0031508 - pericentric heterochromatin formation

References:

GO:0031146 - SCF-dependent proteasomal ubiquitin-dependent protein catabolic process

References:

GO:0030466 - silent mating-type cassette heterochromatin formation

References:

GO:0031509 - subtelomeric heterochromatin formation

References:

GO cellular component

GO:0043494 - CLRC complex

References:

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0043224 - nuclear SCF ubiquitin ligase complex

References:

GO:0005634 - nucleus

References:

GO:0005721 - pericentric heterochromatin

References:

GO molecular function

GO:0097602 - cullin family protein binding

References:

GO:0140851 - histone H3K14 ubiquitin ligase activity

References:

GO:0005515 - protein binding

References:

GO:0061630 - ubiquitin protein ligase activity

References:

GO:0008270 - zinc ion binding

References:

Modification

MOD:01149 - sumoylated lysine

References:

MOD:01148 - ubiquitinylated lysine

References:

Protein features

PBO:0111749 - ubiquitin-protein ligase E3

PBO:0111746 - zf-C3HC4 type (RING finger)

PBO:0111743 - zinc finger protein

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF12678zf-rbx1Znf_RING_H2Pfam
cd16485mRING-H2-C3H2C2D_RBX1CDD
PS50089ZF_RING_2Znf_RINGPROSITE profiles
G3DSA:3.30.40.10:FF:000010CATH-FunFam
G3DSA:3.30.40.10Znf_RING/FYVE/PHDCATH-Gene3D
SSF57850SUPERFAMILY
PTHR11210RING-box_E3_Ubiquitin_LigasePANTHER

Orthologs

References / Literature

PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:11337588 - Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome.
Lyapina S et al. Science 2001 May 18;292(5520):1382-5
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:32047038 - Basis for metabolite-dependent Cullin-RING ligase deneddylation by the COP9 signalosome.
Lin H et al. Proc Natl Acad Sci U S A 2020 Feb 25;117(8):4117-4124
PMID:12167173 - Combinatorial diversity of fission yeast SCF ubiquitin ligases by homo- and heterooligomeric assemblies of the F-box proteins Pop1p and Pop2p.
Seibert V et al. BMC Biochem 2002 Aug 07;3:22
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:25165823 - Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.
Tsutsui Y et al. PLoS Genet 2014 Aug;10(8):e1004542
GO_REF:0000002 - Comments
PMID:23535663 - CAND1 controls in vivo dynamics of the cullin 1-RING ubiquitin ligase repertoire.
Wu S et al. Nat Commun 2013;4:1642
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:17114925 - A cullin E3 ubiquitin ligase complex associates with Rik1 and the Clr4 histone H3-K9 methyltransferase and is required for RNAi-mediated heterochromatin formation.
Hong EJ et al. RNA Biol 2005;2(3):106-11
PMID:24449894 - CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing.
Kuscu C et al. Proc Natl Acad Sci U S A 2014 Feb 04;111(5):1795-800
PMID:31468675 - H3K14 ubiquitylation promotes H3K9 methylation for heterochromatin assembly.
Oya E et al. EMBO Rep 2019 Oct 04;20(10):e48111
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:14527422 - BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases.
Geyer R et al. Mol Cell 2003 Sep;12(3):783-90
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:16024659 - A Rik1-associated, cullin-dependent E3 ubiquitin ligase is essential for heterochromatin formation.
Horn PJ et al. Genes Dev 2005 Jul 15;19(14):1705-14
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663