protein coding gene - mrp4 (SPAC24C9.10c) - mitochondrial ribosomal protein subunit S2

Gene summary

Standard name: mrp4
Systematic ID: SPAC24C9.10c
Product: mitochondrial ribosomal protein subunit S2
Organism: Schizosaccharomyces pombe (fission yeast)
UniProt ID: O13970
ORFeome ID: 10/10A05
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome I: 3065326..3066266 reverse strand

Annotation

Disease association

MONDO:0054781 - combined oxidative phosphorylation deficiency 36

References:

PB_REF:0000006

GO biological process

GO:0032543 - mitochondrial translation

References:

GO_REF:0000024

GO cellular component

GO:0005763 - mitochondrial small ribosomal subunit

References:

GO_REF:0000033

GO:0005739 - mitochondrion

References:

PMID:16823372

GO molecular function

GO:0003735 - structural constituent of ribosome

References:

GO_REF:0000033

GO:0000049 - tRNA binding

References:

GO_REF:0000024

Modification

MOD:01148 - ubiquitinylated lysine

References:

PMID:37970674

Protein sequence feature

SO:0001808 - mitochondrial_targeting_signal

References:

PMID:36408920

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0004481 - abolished cell population growth at high temperature

References:

PMID:33823662

Genotypes:

mrp4-77(E197K aa)

FYPO:0004944 - decreased mitochondrial membrane potential

References:

PMID:33823662

Genotypes:

mrp4-77(E197K aa)

FYPO:0007610 - decreased mitochondrial volume

References:

PMID:33823662

Genotypes:

mrp4-77(E197K aa)

FYPO:0008128 - decreased vegetative cell population growth on low glucose

References:

PMID:33823662

Genotypes:

mrp4-77(E197K aa)

FYPO:0002111 - inviable tapered vegetative cell

References:

PMID:23697806

Genotypes:

mrp4delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

mrp4delta

FYPO:0002199 - inviable vegetative cell with normal cell morphology

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

PMID:33823662

Genotypes:

mrp4-77(E197K aa)

FYPO:0003896 - normal mitochondrial morphology

References:

PMID:33823662

Genotypes:

mrp4-77(E197K aa)

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF00318	Ribosomal_S2	Ribosomal_uS2	PFAM
cd01425	RPS2	Ribosomal_uS2	CDD
PS00962	RIBOSOMAL_S2_1	Ribosomal_uS2_CS	PROSITE_PATTERNS
PR00395	RIBOSOMALS2	Ribosomal_uS2	PRINTS
G3DSA:3.40.50.10490:FF:000050			FUNFAM
SSF52313	Ribosomal protein S2	Ribosomal_uS2_flav_dom_sf	SUPERFAMILY
G3DSA:3.40.50.10490			GENE3D
PTHR12534	30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR	Ribosomal_uS2_bac/mit/plastid	PANTHER
MF_00291_B	Ribosomal_uS2_B	Ribosomal_uS2_bac/mit/plastid	HAMAP
TIGR01011	rpsB_bact	Ribosomal_uS2_bac/mit/plastid	NCBIFAM

Orthologs

MRPS2 (HGNC:14495) Homo sapiens
mrp4 (SJAG_03584) Schizosaccharomyces japonicus
MRP4 (YHL004W) Saccharomyces cerevisiae

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.: Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.: UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:33823662 - Multiple nutritional phenotypes of fission yeast mutants defective in genes encoding essential mitochondrial proteins.: Uehara L et al. Open Biol 2021 Apr;11(4):200369
PMID:34119521 - Schizosaccharomyces pombe Ppr10 and Mpa1 together mediate mitochondrial translational initiation.: Luo Y et al. J Biol Chem 2021 Jul;297(1):100869
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23892058 - Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria.: Dujeancourt L et al. Mitochondrion 2013 Nov;13(6):871-80