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protein coding gene - cul3 (SPAC24H6.03) - cullin 3

Gene summary

Standard name
cul3
Systematic ID
SPAC24H6.03
Product
cullin 3
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
pcu3
UniProt ID
Q09760
ORFeome ID
40/40H10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 485723..488714 reverse strand

Annotation

Disease association

MONDO:0030994 - neurodevelopmental disorder with or without autism or seizures

References:

MONDO:0013782 - pseudohypoaldosteronism type 2E

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GO biological process

GO:0043161 - proteasome-mediated ubiquitin-dependent protein catabolic process

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GO cellular component

GO:0031463 - Cul3-RING ubiquitin ligase complex

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GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0005515 - protein binding

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GO:0160072 - ubiquitin ligase complex scaffold activity

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GO:0031625 - ubiquitin protein ligase binding

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Modification

MOD:01150 - neddylated lysine

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MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

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Genotypes:

FYPO:0001122 - elongated vegetative cell

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Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

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Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0001023 - normal growth on cisplatin

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Genotypes:

FYPO:0002800 - normal protein degradation during vegetative growth

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001098 - sensitive to 4-nitroquinoline N-oxide

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Genotypes:

FYPO:0000093 - sensitive to arsenic

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0000091 - sensitive to thiabendazole

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Genotypes:

FYPO:0000268 - sensitive to UV during vegetative growth

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Genotypes:

FYPO:0001492 - viable elongated vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002104 - viable vegetative cell with normal cell shape

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF26557Cullin_ABCullin-like_ABPFAM
PF10557Cullin_Nedd8Cullin_neddylation_domainPFAM
PF00888CullinCullin_NPFAM
PS01256CULLIN_1Cullin_CSPROSITE_PATTERNS
PS50069CULLIN_2Cullin_homologyPROSITE_PROFILES
SM00884Cullin_Nedd8_2Cullin_neddylation_domainSMART
SM00182cul_2Cullin_homologySMART
G3DSA:1.20.1310.10:FF:000002FUNFAM
G3DSA:3.30.230.130:FF:000011FUNFAM
G3DSA:1.20.1310.10:FF:000001FUNFAM
G3DSA:1.10.10.10:FF:000014FUNFAM
SSF46785"Winged helix" DNA-binding domainWH_DNA-bd_sfSUPERFAMILY
SSF75632Cullin homology domainCullin_homology_sfSUPERFAMILY
SSF74788Cullin repeat-likeCullin_repeat-like_dom_sfSUPERFAMILY
G3DSA:1.10.10.10WH-like_DNA-bd_sfGENE3D
G3DSA:3.30.230.130Cullin; Chain C, Domain 2GENE3D
G3DSA:1.20.1310.10Cullin RepeatsGENE3D
PTHR11932CULLINCullinPANTHER
CoilCoilCOILS

Orthologs

References / Literature

PMID:9990507 - Two F-box/WD-repeat proteins Pop1 and Pop2 form hetero- and homo-complexes together with cullin-1 in the fission yeast SCF (Skp1-Cullin-1-F-box) ubiquitin ligase.
Kominami K et al. Genes Cells 1998 Nov;3(11):721-35
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:15769255 - Nep1, a Schizosaccharomyces pombe deneddylating enzyme.
Zhou L et al. Biochem J 2005 Jul 15;389(Pt 2):307-14
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20625380 - A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length.
Liu NN et al. Cell Res 2010 Aug;20(8):963-5
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:14527422 - BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases.
Geyer R et al. Mol Cell 2003 Sep;12(3):783-90
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16252005 - Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase.
Liu C et al. EMBO J 2005 Nov 16;24(22):3940-51
PMID:12718879 - Fission yeast COP9/signalosome suppresses cullin activity through recruitment of the deubiquitylating enzyme Ubp12p.
Zhou C et al. Mol Cell 2003 Apr;11(4):927-38
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:11504566 - The fission yeast COP9/signalosome is involved in cullin modification by ubiquitin-related Ned8p.
Zhou C et al. BMC Biochem 2001;2:7
PMID:21247416 - Ubiquitin-proteasome genes as targets for modulation of cisplatin sensitivity in fission yeast.
Gatti L et al. BMC Genomics 2011 Jan 19;12:44
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:27558664 - Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.
Guo L et al. G3 (Bethesda) 2016 Oct 13;6(10):3317-3333
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:21760946 - Identification of genes affecting the toxicity of anti-cancer drug bortezomib by genome-wide screening in S. pombe.
Takeda K et al. PLoS One 2011;6(7):e22021
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs