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protein coding gene - sld3 (SPAC24H6.06) - DNA replication pre-initiation complex subunit Sld3

Gene summary

Standard name
sld3
Systematic ID
SPAC24H6.06
Product
DNA replication pre-initiation complex subunit Sld3
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
mug175
UniProt ID
Q09761
ORFeome ID
37/37E10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 476474..478724 reverse strand

Annotation

GO biological process

GO:1902975 - mitotic DNA replication initiation

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0031261 - DNA replication preinitiation complex

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GO molecular function

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Qualitative gene expression

PomGeneEx:0000026 - ribosomal density increased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

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Genotypes:

FYPO:0006728 - abolished mitotic DNA replication initiation

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Genotypes:

FYPO:0005095 - abolished protein localization to chromatin at replication origin

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Genotypes:

FYPO:0002033 - abolished protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0003950 - decreased protein localization to chromatin at replication origin

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

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Genotypes:

FYPO:0001974 - increased number of cells with 1C DNA content

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Genotypes:

FYPO:0004961 - increased protein localization to chromatin at replication origin

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Genotypes:

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

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Genotypes:

FYPO:0002724 - inviable after spore germination, single or double cell division, elongated cell

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Genotypes:

FYPO:0002059 - inviable cell population

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0001839 - normal minichromosome loss

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001701 - sensitive to bortezomib

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

Protein features

IDNameInterPro nameDB name
PF08639Sld3_STDDNA_replication_reg_Sld3_CPfam
G3DSA:1.20.58.2130CATH-Gene3D
PTHR28067Sld3PANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.
Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53
PMID:15194812 - A novel intermediate in initiation complex assembly for fission yeast DNA replication.
Yamada Y et al. Mol Biol Cell 2004 Aug;15(8):3740-50
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:22987637 - Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast.
Tazumi A et al. Genes Dev 2012 Sep 15;26(18):2050-62
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:21760946 - Identification of genes affecting the toxicity of anti-cancer drug bortezomib by genome-wide screening in S. pombe.
Takeda K et al. PLoS One 2011;6(7):e22021
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:16990792 - Ordered assembly of Sld3, GINS and Cdc45 is distinctly regulated by DDK and CDK for activation of replication origins.
Yabuuchi H et al. EMBO J 2006 Oct 04;25(19):4663-74
PMID:21945095 - Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1).
Taylor M et al. DNA Repair (Amst) 2011 Nov 10;10(11):1154-63
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:22380713 - Treslin, DUE-B, and GEMC1 cannot complement Sld3 mutants in fission yeast.
Wang Z et al. FEMS Yeast Res 2012 Jun;12(4):486-90
PMID:30295604 - BRCT domains of the DNA damage checkpoint proteins TOPBP1/Rad4 display distinct specificities for phosphopeptide ligands.
Day M et al. Elife 2018 Oct 08;7
PMID:21593208 - CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast.
Fukuura M et al. Mol Biol Cell 2011 Jul 15;22(14):2620-33
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:12006645 - SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast.
Nakajima R et al. Mol Biol Cell 2002 May;13(5):1462-72
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6