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protein coding gene - gef1 (SPAC24H6.09) - RhoGEF for Cdc42 (Gef1)

Gene summary

Standard name
gef1
Systematic ID
SPAC24H6.09
Product
RhoGEF for Cdc42 (Gef1)
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09763
ORFeome ID
38/38C05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 469981..472990 reverse strand

Annotation

Disease association

MONDO:0032735 - cataract 48

References:

GO biological process

GO:0072583 - clathrin-dependent endocytosis

References:

GO:0061245 - establishment or maintenance of bipolar cell polarity

References:

GO:0051518 - positive regulation of bipolar cell growth

References:

GO:1903473 - positive regulation of mitotic actomyosin contractile ring contraction

References:

GO:0032955 - regulation of division septum assembly

References:

GO:2000114 - regulation of establishment of cell polarity

References:

GO:2000100 - regulation of establishment or maintenance of bipolar cell polarity regulating cell shape

References:

GO cellular component

GO:0051285 - cell cortex of cell tip

References:

GO:1902716 - cell cortex of growing cell tip

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GO:0032153 - cell division site

References:

GO:0051286 - cell tip

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GO:0005737 - cytoplasm

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GO:0005829 - cytosol

References:

GO:0000935 - division septum

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GO:0061645 - endocytic patch

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GO:0031097 - medial cortex

References:

GO:0110085 - mitotic actomyosin contractile ring

References:

GO molecular function

GO:0005085 - guanyl-nucleotide exchange factor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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Multi-locus phenotype

FYPO:0000164 - abnormal cell separation after cytokinesis

References:

Genotypes:

FYPO:0005503 - abnormally monopolar protein localization to cell tip

References:

Genotypes:

FYPO:0004105 - abolished polar cell growth

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Genotypes:

FYPO:0001880 - abolished protein localization to cell division site

References:

Genotypes:

FYPO:0006938 - actin cables present in increased numbers during cytokinesis

References:

Genotypes:

FYPO:0006939 - asymmetric actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0006940 - asymmetric protein localization to actomyosin contractile ring

References:

Genotypes:

FYPO:0004572 - decreased exocytosis during vegetative growth

References:

Genotypes:

FYPO:0004470 - decreased protein localization to actomyosin contractile ring during mitotic anaphase

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Genotypes:

FYPO:0002869 - decreased protein localization to cell division site

References:

Genotypes:

FYPO:0001586 - decreased protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0001365 - decreased rate of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0005022 - decreased rate of protein localization to actomyosin contractile ring

References:

Genotypes:

FYPO:0000729 - delayed onset of actomyosin contractile ring assembly

References:

Genotypes:

FYPO:0004653 - delayed onset of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0003946 - delayed onset of protein localization to cell division site

References:

Genotypes:

FYPO:0002021 - dispersed actin cortical patch localization during vegetative growth

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Genotypes:

FYPO:0000223 - elongated multiseptate vegetative cell

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Genotypes:

FYPO:0003533 - increased bipolar index

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Genotypes:

FYPO:0006941 - increased duration of protein localization to actin cortical patch

References:

Genotypes:

FYPO:0003532 - increased monopolar index

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Genotypes:

FYPO:0006081 - increased protein localization to actin cortical patch

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Genotypes:

FYPO:0002150 - inviable spore population

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Genotypes:

FYPO:0002191 - inviable swollen septated spheroid vegetative cell

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Genotypes:

FYPO:0001252 - multinucleate multiseptate vegetative cell

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Genotypes:

FYPO:0000118 - multiseptate vegetative cell

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Genotypes:

FYPO:0005465 - normal cell polarity

References:

Genotypes:

FYPO:0005906 - normal onset of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0006008 - normal onset of protein localization to cell division site

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Genotypes:

FYPO:0000744 - normal protein localization to actin cortical patch

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Genotypes:

FYPO:0006876 - normal protein localization to cell cortex of cell tip

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Genotypes:

FYPO:0003316 - normal protein localization to growing cell tip

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Genotypes:

FYPO:0007239 - normal protein localization to old growing cell tip

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Genotypes:

FYPO:0006639 - normal vegetative cell diameter

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0007829 - premature protein localization to cell division site

References:

Genotypes:

FYPO:0000107 - sensitive to latrunculin A

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0006616 - viable vegetative cell with increased cell diameter

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Genotypes:

FYPO:0002104 - viable vegetative cell with normal cell shape

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Genotypes:

Protein features

PBO:0111772 - BAR domain

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0005503 - abnormally monopolar protein localization to cell tip

References:

Genotypes:

FYPO:0007242 - abolished NETO in both daughter cells

References:

Genotypes:

FYPO:0007241 - abolished NETO in cell derived from growing end and premature NETO in cell derived from non-growing end

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Genotypes:

FYPO:0001880 - abolished protein localization to cell division site

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Genotypes:

FYPO:0002859 - abolished protein localization to non-growing cell tip

References:

Genotypes:

FYPO:0006009 - actin cables present in decreased numbers during cytokinesis

References:

Genotypes:

FYPO:0008389 - activation of monopolar cell growth at old end during septation

References:

Genotypes:

FYPO:0006939 - asymmetric actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0003535 - decreased bipolar index

References:

Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0002869 - decreased protein localization to cell division site

References:

Genotypes:

FYPO:0002719 - decreased protein localization to septum

References:

Genotypes:

FYPO:0005803 - decreased rate of protein movement within plasma membrane at cell side

References:

Genotypes:

FYPO:0004653 - delayed onset of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0003946 - delayed onset of protein localization to cell division site

References:

Genotypes:

FYPO:0002021 - dispersed actin cortical patch localization during vegetative growth

References:

Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

References:

Genotypes:

FYPO:0003533 - increased bipolar index

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0009076 - increased cell population growth on sucrose carbon source

References:

Genotypes:

FYPO:0006941 - increased duration of protein localization to actin cortical patch

References:

Genotypes:

FYPO:0002527 - increased duration of protein localization to cell division site

References:

Genotypes:

FYPO:0003532 - increased monopolar index

References:

Genotypes:

FYPO:0006081 - increased protein localization to actin cortical patch

References:

Genotypes:

FYPO:0004977 - increased protein localization to new cell tip

References:

Genotypes:

FYPO:0001406 - increased septum thickness

References:

Genotypes:

FYPO:0003842 - inviable following spore germination, single cell division, abolished mitotic sister chromatid separation

References:

Genotypes:

FYPO:0005020 - normal duration of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0004646 - normal duration of mitotic anaphase

References:

Genotypes:

FYPO:0006007 - normal duration of protein localization to cell division site

References:

Genotypes:

FYPO:0007243 - normal NETO in cell derived from growing end and premature NETO in cell derived from non-growing end

References:

Genotypes:

FYPO:0005905 - normal onset of actomyosin contractile ring assembly

References:

Genotypes:

FYPO:0006008 - normal onset of protein localization to cell division site

References:

Genotypes:

FYPO:0000644 - normal protein localization during vegetative growth

References:

Genotypes:

FYPO:0002559 - normal protein localization to actomyosin contractile ring

References:

Genotypes:

FYPO:0001587 - normal protein localization to cell tip during vegetative growth

References:

Genotypes:

FYPO:0007239 - normal protein localization to old growing cell tip

References:

Genotypes:

FYPO:0004895 - normal rate of actomyosin contractile ring contraction

References:

Genotypes:

FYPO:0005805 - normal rate of protein movement within plasma membrane at cell tip

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0007829 - premature protein localization to cell division site

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000107 - sensitive to latrunculin A

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0000022 - shmoo with long tip

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

FYPO:0000024 - stubby vegetative cell

References:

Genotypes:

FYPO:0001877 - viable thin vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0001510 - viable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0009798 - faster evolving duplicate

Protein features

IDNameInterPro nameDB name
PF00621RhoGEFDH_domPfam
cd00160RhoGEFDH_domCDD
PS50010DH_2DH_domPROSITE profiles
SM00325RhoGEFDH_domSMART
G3DSA:1.20.900.10:FF:000038CATH-FunFam
G3DSA:1.20.900.10DBL_dom_sfCATH-Gene3D
SSF48065DBL_dom_sfSUPERFAMILY
PTHR22834Dynamin-Rho_GEFPANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:39509469 - Rho1 and Rgf1 establish a new actin-dependent signal to determine growth poles in yeast independently of microtubules and the Tea1-Tea4 complex.
Garcia P et al. PLoS Biol 2024 Nov 07;22(11):e3002491
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:37039135 - Cdc42 prevents precocious Rho1 activation during cytokinesis in a Pak1-dependent manner.
Onwubiko UN et al. J Cell Sci 2023 Apr 15;136(8)
PMID:25837586 - Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking.
Bendezú FO et al. PLoS Biol 2015 Apr;13(4):e1002097
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:12972551 - Gef1p and Scd1p, the Two GDP-GTP exchange factors for Cdc42p, form a ring structure that shrinks during cytokinesis in Schizosaccharomyces pombe.
Hirota K et al. Mol Biol Cell 2003 Sep;14(9):3617-27
PMID:19646873 - The conserved NDR kinase Orb6 controls polarized cell growth by spatial regulation of the small GTPase Cdc42.
Das M et al. Curr Biol 2009 Aug 11;19(15):1314-9
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:31666919 - Rho Family GTPases in Fission Yeast Cytokinesis.
Hercyk B et al. Commun Integr Biol 2019;12(1):171-180
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:26246599 - Phosphorylation-dependent inhibition of Cdc42 GEF Gef1 by 14-3-3 protein Rad24 spatially regulates Cdc42 GTPase activity and oscillatory dynamics during cell morphogenesis.
Das M et al. Mol Biol Cell 2015 Oct 01;26(19):3520-34
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:29249658 - Mechanisms Connecting the Conserved Protein Kinases Ssp1, Kin1, and Pom1 in Fission Yeast Cell Polarity and Division.
Lee ME et al. Curr Biol 2018 Jan 08;28(1):84-92.e4
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:34523683 - Cdc42 reactivation at growth sites is regulated by local cell-cycle-dependent loss of its GTPase-activating protein Rga4 in fission yeast.
Rich-Robinson J et al. J Cell Sci 2021 Oct 01;134(19)
PMID:18256290 - Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis.
Pinar M et al. Mol Biol Cell 2008 Apr;19(4):1727-38
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:26941334 - Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis.
Wei B et al. Mol Biol Cell 2016 Apr 15;27(8):1235-45
PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.
Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787
PMID:28945192 - Regulated Ire1-dependent mRNA decay requires no-go mRNA degradation to maintain endoplasmic reticulum homeostasis in S. pombe .
Guydosh NR et al. Elife 2017 Sep 25;6
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:24146635 - Hsp70-Hsp40 chaperone complex functions in controlling polarized growth by repressing Hsf1-driven heat stress-associated transcription.
Vjestica A et al. PLoS Genet 2013;9(10):e1003886
PMID:24554432 - The Tea4-PP1 landmark promotes local growth by dual Cdc42 GEF recruitment and GAP exclusion.
Kokkoris K et al. J Cell Sci 2014 May 01;127(Pt 9):2005-16
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:12529446 - Gef1p, a new guanine nucleotide exchange factor for Cdc42p, regulates polarity in Schizosaccharomyces pombe.
Coll PM et al. Mol Biol Cell 2003 Jan;14(1):313-23
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:25411334 - The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis.
Wang N et al. Mol Biol Cell 2015 Jan 15;26(2):238-55
PMID:30709916 - A Cdc42 GEF, Gef1, through endocytosis organizes F-BAR Cdc15 along the actomyosin ring and promotes concentric furrowing.
Onwubiko UN et al. J Cell Sci 2019 Feb 28;132(5)
PMID:29930085 - Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.
Tay YD et al. J Cell Sci 2018 Jul 19;131(14)
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:31591131 - F-BAR Cdc15 Promotes Cdc42 Activation During Cytokinesis and Cell Polarization in Schizosaccharomyces pombe .
Hercyk BS et al. Genetics 2019 Dec;213(4):1341-1356
PMID:31719163 - A novel interplay between GEFs orchestrates Cdc42 activity during cell polarity and cytokinesis in fission yeast.
Hercyk BS et al. J Cell Sci 2019 Dec 03;132(23)
PMID:32915139 - Stress-activated MAPK signaling controls fission yeast actomyosin ring integrity by modulating formin For3 levels.
Gómez-Gil E et al. Elife 2020 Sep 11;9
PMID:39012625 - Arp2/3-dependent endocytosis ensures Cdc42 oscillations by removing Pak1-mediated negative feedback.
Harrell MA et al. J Cell Biol 2024 Sep 02;223(10)
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:17363901 - Hob3p, the fission yeast ortholog of human BIN3, localizes Cdc42p to the division site and regulates cytokinesis.
Coll PM et al. EMBO J 2007 Apr 04;26(7):1865-77
PMID:21849474 - Spatial control of Cdc42 activation determines cell width in fission yeast.
Kelly FD et al. Mol Biol Cell 2011 Oct;22(20):3801-11
PMID:24947517 - The putative exchange factor Gef3p interacts with Rho3p GTPase and the septin ring during cytokinesis in fission yeast.
Muñoz S et al. J Biol Chem 2014 Aug 08;289(32):21995-2007