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protein coding gene - uba3 (SPAC24H6.12c) - NEDD8 activating enzyme E1-type Uba3

Gene summary

Standard name
uba3
Systematic ID
SPAC24H6.12c
Product
NEDD8 activating enzyme E1-type Uba3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q09765
ORFeome ID
20/20F06
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome I: 461716..463599 forward strand

Annotation

GO biological process

GO:0045116 - protein neddylation

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0019781 - NEDD8 activating enzyme activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

References:

Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00899ThiFThiF_NAD_FAD-bdPfam
PF08825E2_bindE2-bindingPfam
cd01488Uba3_RUBUba3_NCDD
PS00865UBIQUITIN_ACTIVAT_2UBQ-activ_enz_E1_Cys_ASPROSITE patterns
SM01181E2_bindE2-bindingSMART
G3DSA:1.10.10.520:FF:000009CATH-FunFam
G3DSA:1.10.10.520Ub_act_enz_dom_a_sfCATH-Gene3D
G3DSA:3.10.290.20CATH-Gene3D
G3DSA:3.40.50.720CATH-Gene3D
SSF69572Ubiquitin-activating_enzSUPERFAMILY
PTHR10953ThiF/MoeB/HesAPANTHER

Orthologs

References / Literature

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:10880460 - Covalent modifier NEDD8 is essential for SCF ubiquitin-ligase in fission yeast.
Osaka F et al. EMBO J 2000 Jul 03;19(13):3475-84
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:14623327 - But1 and But2 proteins bind to Uba3, a catalytic subunit of E1 for neddylation, in fission yeast.
Yashiroda H et al. Biochem Biophys Res Commun 2003 Nov 21;311(3):691-5
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.
Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22673520 - Constitutively active Cullin-RING-Ligases fail to rescue loss of NEDD8 conjugation in Schizosaccharomyces pombe.
Girdwood D et al. FEBS Lett 2012 May 21;586(10):1522-8