PomBase home

protein coding gene - fft3 (SPAC25A8.01c) - histone chaperone/ATP-dependent chromatin remodeller, SMARCAD1 family Fft3

Gene summary

Standard name
fft3
Systematic ID
SPAC25A8.01c
Product
histone chaperone/ATP-dependent chromatin remodeller, SMARCAD1 family Fft3
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
snf2SR
UniProt ID
O42861
ORFeome ID
47/47C01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 2098195..2101805 reverse strand

Annotation

Disease association

MONDO:0007507 - absence of fingerprints-congenital milia syndrome

References:

MONDO:0007619 - isolated congenital adermatoglyphia

References:

MONDO:0008416 - palmoplantar keratoderma-sclerodactyly syndrome

References:

GO biological process

GO:0140698 - attachment of telomeric heterochromatin to nuclear envelope

References:

GO:0006338 - chromatin remodeling

References:

GO:0000729 - DNA double-strand break processing

References:

GO:0140861 - DNA repair-dependent chromatin remodeling

References:

GO:0031507 - heterochromatin formation

References:

GO:0045944 - positive regulation of transcription by RNA polymerase II

References:

GO:0006357 - regulation of transcription by RNA polymerase II

References:

GO:0031297 - replication fork processing

References:

GO:0140673 - transcription elongation-coupled chromatin remodeling

References:

GO:0010526 - transposable element silencing

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0000792 - heterochromatin

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0140665 - ATP-dependent H3-H4 histone complex chaperone activity

References:

GO:0003682 - chromatin binding

References:

GO:0003684 - damaged DNA binding

References:

GO:0140713 - histone chaperone activity

References:

GO:0140750 - nucleosome array spacer activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0004790 - abnormal telomere-nuclear envelope distance during vegetative growth

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0004604 - decreased chromatin silencing at subtelomere

References:

Genotypes:

FYPO:0005948 - decreased protein localization to chromatin at highly transcribed RNA polymerase II genes during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0005950 - increased nucleosome occupancy at highly transcribed RNA polymerase II genes

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0005952 - normal nucleosome occupancy at highly transcribed RNA polymerase II genes

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000084 - sensitive to 6-azauracil

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0000112 - sensitive to sorbitol

References:

Genotypes:

Protein features

PBO:0111843 - CUE domain protein

PBO:0111853 - fun thirty family

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000427 - abnormal G1 to G0 transition

References:

Genotypes:

FYPO:0003586 - abnormal replication fork processing

References:

Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

References:

Genotypes:

FYPO:0007376 - abolished epigenetic heterochromatin inheritance

References:

Genotypes:

FYPO:0001946 - abolished mitotic sister chromatid separation

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009073 - decreased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0003740 - decreased CENP-A containing chromatin assembly

References:

Genotypes:

FYPO:0003217 - decreased chromatin silencing at centromere central core

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

References:

Genotypes:

FYPO:0002827 - decreased chromatin silencing at silent mating-type cassette

References:

Genotypes:

FYPO:0004604 - decreased chromatin silencing at subtelomere

References:

Genotypes:

FYPO:0006686 - decreased DNA double-strand break processing

References:

Genotypes:

FYPO:0006318 - decreased DNA resection during replication fork processing

References:

Genotypes:

FYPO:0005922 - decreased heterochromatin assembly at subtelomere

References:

Genotypes:

FYPO:0005433 - decreased meiotic recombination at hotspot

References:

Genotypes:

FYPO:0005948 - decreased protein localization to chromatin at highly transcribed RNA polymerase II genes during vegetative growth

References:

Genotypes:

FYPO:0004345 - decreased protein localization to chromatin at long terminal repeat

References:

Genotypes:

FYPO:0005918 - decreased protein localization to subtelomeric heterochromatin

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0005924 - increased H2A.Z level at centromere inner repeat

References:

Genotypes:

FYPO:0005923 - increased H2A.Z level at subtelomere

References:

Genotypes:

FYPO:0000893 - increased histone H3-K9 acetylation at centromere during vegetative growth

References:

Genotypes:

FYPO:0005921 - increased histone H4-K12 acetylation at centromere inner repeat during vegetative growth

References:

Genotypes:

FYPO:0005316 - increased histone H4-K12 acetylation at subtelomere during vegetative growth

References:

Genotypes:

FYPO:0003248 - increased level of histone H3 at centromere inner repeat

References:

Genotypes:

FYPO:0005371 - increased linear minichromosome loss during vegetative growth

References:

Genotypes:

FYPO:0005950 - increased nucleosome occupancy at highly transcribed RNA polymerase II genes

References:

Genotypes:

FYPO:0005917 - increased subtelomeric heterochromatin RNA level

References:

Genotypes:

FYPO:0000238 - inviable cell upon G0 to G1 transition

References:

Genotypes:

FYPO:0002303 - inviable mononucleate monoseptate vegetative cell with anucleate compartment

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0006660 - loss of viability upon G0 to G1 transition

References:

Genotypes:

FYPO:0005517 - normal growth on 6-azauracil

References:

Genotypes:

FYPO:0000961 - normal growth on sorbitol

References:

Genotypes:

FYPO:0005920 - normal nucleosome occupancy at tRNA genes

References:

Genotypes:

FYPO:0004314 - normal protein localization to CENP-A containing chromatin

References:

Genotypes:

FYPO:0005087 - normal protein localization to chromatin at tRNA gene

References:

Genotypes:

FYPO:0007254 - normal replication fork processing

References:

Genotypes:

FYPO:0004924 - normal telomere tethering at nuclear periphery during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

References:

Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000077 - resistance to rapamycin

References:

Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0009067 - sensitive to amorolfine

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000096 - sensitive to cadmium

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0009069 - sensitive to ciclopirox olamine

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0003358 - sensitive to miconazole

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00176SNF2-rel_domSNF2_NPFAM
PF00271Helicase_CHelicase_C-likePFAM
cd18793SF2_C_SNFSNF2/RAD54-like_CCDD
cd17998DEXHc_SMARCAD1CDD
PS51192HELICASE_ATP_BIND_1Helicase_ATP-bdPROSITE_PROFILES
PS51194HELICASE_CTERHelicase_C-likePROSITE_PROFILES
SM00490helicmild6Helicase_C-likeSMART
SM00487ultradead3Helicase_ATP-bdSMART
G3DSA:3.40.50.10810:FF:000014FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
G3DSA:3.40.50.10810SNF2-like_sfGENE3D
PTHR10799SNF2/RAD54 HELICASE FAMILYPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMOBIDB-Negative-Polyelectrolyte

Orthologs

References / Literature

PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:18422602 - Schizosaccharomyces pombe Snf2SR, a novel SNF2 family protein, interacts with Ran GTPase and modulates both RanGEF and RanGAP activities.
Ohba T et al. Genes Cells 2008 Jun;13(6):571-82
PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.
Han TX et al. Genome Biol 2010;11(6):R60
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23
PMID:19672306 - Genome-wide screen of genes required for caffeine tolerance in fission yeast.
Calvo IA et al. PLoS One 2009 Aug 12;4(8):e6619
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:21965289 - Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B.
Buttrick GJ et al. Mol Biol Cell 2011 Dec;22(23):4486-502
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.
Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39
PMID:36793083 - The SAGA histone acetyltransferase module targets SMC5/6 to specific genes.
Mahrik L et al. Epigenetics Chromatin 2023 Feb 16;16(1):6
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
GO_REF:0000002 - Comments
PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72
PMID:32499408 - The molecular chaperone Hsp90 regulates heterochromatin assembly through stabilizing multiple complexes in fission yeast.
Sun L et al. J Cell Sci 2020 Jul 07;133(13)
PMID:26902262 - Regulating retrotransposon activity through the use of alternative transcription start sites.
Persson J et al. EMBO Rep 2016 May;17(5):753-68
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:35970865 - The Mis6 inner kinetochore subcomplex maintains CENP-A nucleosomes against centromeric non-coding transcription during mitosis.
Hirai H et al. Commun Biol 2022 Aug 15;5(1):818
PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.
Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:30373637 - Chromatin-mediated regulators of meiotic recombination revealed by proteomics of a recombination hotspot.
Storey AJ et al. Epigenetics Chromatin 2018 Oct 29;11(1):64
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:21437270 - The FUN30 chromatin remodeler, Fft3, protects centromeric and subtelomeric domains from euchromatin formation.
Strålfors A et al. PLoS Genet 2011 Mar;7(3):e1001334
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:34731638 - The histone chaperone FACT facilitates heterochromatin spreading by regulating histone turnover and H3K9 methylation states.
Murawska M et al. Cell Rep 2021 Nov 02;37(5):109944
PMID:19111658 - HP1 proteins form distinct complexes and mediate heterochromatic gene silencing by nonoverlapping mechanisms.
Motamedi MR et al. Mol Cell 2008 Dec 26;32(6):778-90
PMID:25798942 - The Fun30 chromatin remodeler Fft3 controls nuclear organization and chromatin structure of insulators and subtelomeres in fission yeast.
Steglich B et al. PLoS Genet 2015 Mar;11(3):e1005101
PMID:28805495 - Shushing histone turnover: It's FUN protecting epigenome-genome.
Taneja N et al. Cell Cycle 2017 Oct 02;16(19):1731-1732
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:31278118 - Cohesin Impedes Heterochromatin Assembly in Fission Yeast Cells Lacking Pds5.
Folco HD et al. Genetics 2019 Sep;213(1):127-141
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:38285941 - Specialized replication of heterochromatin domains ensures self-templated chromatin assembly and epigenetic inheritance.
Nathanailidou P et al. Proc Natl Acad Sci U S A 2024 Feb 06;121(6):e2315596121
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24463365 - Systematic screen for mutants resistant to TORC1 inhibition in fission yeast reveals genes involved in cellular ageing and growth.
Rallis C et al. Biol Open 2014 Feb 15;3(2):161-71
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:30116786 - Genetic regulation of mitotic competence in G 0 quiescent cells.
Sajiki K et al. Sci Adv 2018 Aug;4(8):eaat5685
PMID:15317843 - Schizosaccharomyces pombe RanGAP homolog, SpRna1, is required for centromeric silencing and chromosome segregation.
Kusano A et al. Mol Biol Cell 2004 Nov;15(11):4960-70
PMID:31575705 - Chromatin remodeler Fft3 plays a dual role at blocked DNA replication forks.
Ait-Saada A et al. Life Sci Alliance 2019 Oct;2(5)
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:29343513 - Using genetic buffering relationships identified in fission yeast to reveal susceptibilities in cells lacking hamartin or tuberin function.
Rayhan A et al. Biol Open 2018 Jan 17;7(1)
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:28318821 - SNF2 Family Protein Fft3 Suppresses Nucleosome Turnover to Promote Epigenetic Inheritance and Proper Replication.
Taneja N et al. Mol Cell 2017 Apr 06;66(1):50-62.e6